Figure 4. D2R ablation from the CeA increases avoidance in exploratory tasks. The result of a MANOVA including every measure is shown at the center, right. A, Dark/light box. Left, Time spent in the lit chamber. Student’s t test, t(15) =3.4; p = 0.004. Middle, Latency to first entry into lit chamber in a dark/light box test. Student’s t test; t(15) = −2.3; p = 0.034. Right, Number of entries into the lit chamber in a dark/light box test. GLM with Poisson family (link: log); Wald test, z = −1.7, p = 0.092. B, EPM. Top, Percentage of time on open arms. Only in this measure, the cohort of mice had a significant effect. Therefore, the percentage of time on open arms was analyzed in two different ways (see the Materials and Methods, EPM). Top-left, Percentage of time in open arms, two-way (group × cohort) ANOVA with variance modeling (“VarIdent” function, applied to cohort factor), group, F(1,13) = 8.9, p = 0.011; Cohort, F(1,13) = 19.9, p < 0.001; group × cohort, F(1,13) = 0.16, p = 0.69. Top-right, Percentage of time in open arms normalized to the cohort average. Student’s t test; t(15) = 2.1; p = 0.049). Bottom, Percentage of entries to open arms over total entries (open+closed). Since statistical differences between cohorts were not detected, normalization to cohort averages was not performed. Bottom-left, Percentage of entries to open arms over entries to open and closed arms in an EPM, with data separated according to the cohort. Two-way GLMM with quasi-binomial family (link: logit); Wald test; group, t = −0.31, p = 0.76; cohort, t = 1.86, p = 0.09; group × cohort, t = 0.1, p 0.92. Bottom-right, Percentage of entries to open arms, with data of both cohorts pulled. GLMM with quasi-binomial family (link: logit); Wald test, t = −0.37, p = 0.72. C, Open field, Time in center of the arena during the first 5 min of exposure. Student’s t test, t(15) = 0.35, p = 0.73. CeADrd2KO, n = 9; Ctrl, n = 8 in all experiments. ***p < 0.001, **p < 0.01, *p < 0.05, n.s. p > 0.05.