Figure 6. TAM chow alters the phenotype of surviving cells 35 d after initial exposure. *A*, Experimental model and timeline. Mice were injected with BrdU in the last 3 d of the 14-d TAM chow period. EdU was injected 35 d after initial chow exposure. *B*, Grams of food consumed per mouse (ms) per day over each 3- to 4-d period of diet exposure and TAM consumed in milligrams (mg) (two-way repeated measures ANOVA; day × TAM: *F*_{(3,18)} = 2.322, *p *=* *0.1095; day: *F*_{(1.564,9.384)} = 8.978, *p *=* *0.0088; TAM: *F*_{(1,6)} = 45.86, *p *=* *0.0005; subject: *F*_{(6,18)} = 0.9896, *p *=* *0.4613). Mean ± SEM of *n* = 4 mice. *C*, In female mice, body mass in grams 14 and 35 d after diet initiation (two-way repeated measures ANOVA; day × TAM: *F*_{(1,9)} = 10.62, *p *=* *0.0099; day: *F*_{(1,9)} = 42.05, *p *=* *0.0001; TAM: *F*_{(1,9)} = 0.8454, *p *=* *0.3818; subject: *F*_{(9,9)} = 0.7591, *p *=* *0.6560). Mean ± SEM of *n* = 5–6 mice. *D*, In male mice, body mass in grams 14 and 35 d after diet initiation (two-way repeated measures ANOVA; day × TAM: *F*_{(1,10)} = 18.18, *p *=* *0.0017; day: *F*_{(1,10)} = 62.45, *p *<* *0.0001; TAM: *F*_{(1,10)} = 8.540, *p *=* *0.0152; subject: *F*_{(10,10)} = 1.886, *p *=* *0.1658). Mean ± SEM of *n* = 6 mice. *E*, Density of BrdU+ cells (cells/μm^{2} × 10^{4}) in the dentate gyrus (unpaired *t* test, *t*_{(21)} = 1.675, *p *=* *0.1087). Mean ± SEM of *n* = 11–12 mice. *F*, Density of dentate gyrus BrdU+ cells (cells/μm^{2} × 10^{4}) co-labeled with DCX and/or NeuN (two-way repeated measures ANOVA; cell type × TAM: *F*_{(2,42)} = 8.654, *p *=* *0.0007; cell type: *F*_{(1.207,25.35)} = 83.41, *p *<* *0.0001; TAM: *F*_{(1,21)} = 8.675, *p *=* *0.0077; subject: *F*_{(21,42)} = 1.516, *p *=* *0.1239). Mean ± SEM of *n* = 11–12 mice. *G*, Percent of BrdU+ dentate gyrus cells labeled for DCX and/or NeuN (two-way repeated measures ANOVA; TAM × cell type: *F*_{(2,42)} = 6.501, *p *=* *0.0035; TAM: *F*_{(1,21)} = 16.13, *p *=* *0.0006; cell type: *F*_{(1.545,32.44)} = 151.5, *p *<* *0.0001; subject: *F*_{(21,42)} = 0.3789, *p *=* *0.9903). Mean ± SEM of *n* = 11–12 mice. *H*, Representative images of BrdU, DCX, and NeuN labeling. Scale bar: 20 μm. *I*, Density of EdU+ cells (cells/μm^{2} × 10^{4}) in the dentate gyrus (unpaired *t* test, *t*_{(21)} = 1.043, *p = *0.3090). Mean ± SEM of *n* = 11–12 mice. *J*, Density (cells/μm^{2} × 10^{4}) of dentate gyrus EdU+ phenotypic RGL-NSCs, IPCs, and other cells (two-way repeated measures ANOVA; cell type × TAM: *F*_{(2,42)} = 1.157, *p *=* *0.3241; cell type: *F*_{(1.066,22.38)} = 210.3, *p *<* *0.0001; TAM: *F*_{(1,21)} = 1.087, *p *=* *0.3090; subject: *F*_{(21,42)} = 0.9645, *p *=* *0.5209). Mean ± SEM of *n* = 11–12 mice. *K*, Density (cells/μm^{2} × 10^{4}) of RGL-NSCs and IPCs in the dentate gyrus (two-way repeated measures ANOVA; cell type × TAM: *F*_{(1,21)} = 1.042, *p *=* *0.3191; cell type: *F*_{(1,21)} = 3.794, *p *=* *0.0649; TAM: *F*_{(1,21)} = 2.404, *p *=* *0.1360; subject: *F*_{(21,21)} = 1.138, *p *=* *0.3846). Mean ± SEM of *n* = 11–12 mice. *L*, Representative images of EdU, SOX2, and GFAP labeling. Scale bar: 20 μm. **p *<* *0.05, ***p *<* *0.01, ****p *<* *0.001, *****p *<* *0.0001, as determined by Sidak’s multiple comparisons test.