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Research ArticleResearch Article: New Research, Development

Drosophila Tet Is Required for Maintaining Glial Homeostasis in Developing and Adult Fly Brains

Felice Frey, Jawdat Sandakly, Mirna Ghannam, Caren Doueiry, Fredrik Hugosson, Johannes Berlandi, Joy N. Ismail, Tenzin Gayden, Martin Hasselblatt, Nada Jabado and Margret Shirinian
eNeuro 8 April 2022, 9 (2) ENEURO.0418-21.2022; https://doi.org/10.1523/ENEURO.0418-21.2022
Felice Frey
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
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  • ORCID record for Felice Frey
Jawdat Sandakly
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
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Mirna Ghannam
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
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Caren Doueiry
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
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Fredrik Hugosson
3The Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida, 32080-8610
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Johannes Berlandi
4Institute of Neuropathology, University Hospital Münster, Münster 48149, Germany
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Joy N. Ismail
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
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Tenzin Gayden
2Department of Human Genetics, McGill University, Montreal, Quebec H4A 3J1, Canada
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Martin Hasselblatt
4Institute of Neuropathology, University Hospital Münster, Münster 48149, Germany
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Nada Jabado
2Department of Human Genetics, McGill University, Montreal, Quebec H4A 3J1, Canada
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Margret Shirinian
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0418-21.2022
PubMed 
35396259
Published By 
Society for Neuroscience
History 
  • Received October 5, 2021
  • Revision received March 23, 2022
  • Accepted March 25, 2022
  • Published online April 8, 2022.
Copyright & Usage 
Copyright © 2022 Frey et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Felice Frey1,
  2. Jawdat Sandakly1,
  3. Mirna Ghannam1,
  4. Caren Doueiry1,
  5. Fredrik Hugosson3,
  6. Johannes Berlandi4,
  7. Joy N. Ismail1,
  8. Tenzin Gayden2,
  9. Martin Hasselblatt4,
  10. Nada Jabado2 and
  11. Margret Shirinian1
  1. 1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
  2. 2Department of Human Genetics, McGill University, Montreal, Quebec H4A 3J1, Canada
  3. 3The Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Florida, 32080-8610
  4. 4Institute of Neuropathology, University Hospital Münster, Münster 48149, Germany
  1. Correspondence should be addressed to Margret Shirinian at ms241{at}aub.edu.lb.
View Full Text

Author contributions

  1. Author contributions: F.F., F.H., N.J., and M.S. designed research; F.F., J.S., M.G., C.D., F.H., and J.N.I. performed research; J.B., M.H., and N.J. contributed unpublished reagents/analytic tools; F.F., J.S., M.G., C.D., T.G., and M.S. analyzed data; F.F. and M.S. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This study was supported by the Medical Practice Plan (MPP) at the American University of Beirut, the Lebanese Council for Scientific Research (CNRS) and Matthew Larson Foundation for Pediatric Brain Tumors to N.J. Additionally, this work was made possible thanks to grant #25056 from the Cancer Research Society, Génome Québec and Ministère de l’Économie et de l’Innovation du Québec, which provided salaries and other reagents required for this study. Stocks obtained from the Bloomington Drosophila Stock Center [National Institutes of Health (NIH) Grant P40OD018537] were used in this study. Drosophila antibodies were obtained from the Developmental Studies Hybridoma Bank, created by the National Institute of Child Health and Human Development of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA.

Funding

  • Medical Practice Plan (MPP) at American University of Beirut

  • Lebanese Counsil for Scientific Research (CNRS)

Other Version

  • You are viewing the most recent version of this article.
  • previous version (April 08, 2022).

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Total 2026681209266
Total101762651790
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Drosophila Tet Is Required for Maintaining Glial Homeostasis in Developing and Adult Fly Brains
Felice Frey, Jawdat Sandakly, Mirna Ghannam, Caren Doueiry, Fredrik Hugosson, Johannes Berlandi, Joy N. Ismail, Tenzin Gayden, Martin Hasselblatt, Nada Jabado, Margret Shirinian
eNeuro 8 April 2022, 9 (2) ENEURO.0418-21.2022; DOI: 10.1523/ENEURO.0418-21.2022

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Drosophila Tet Is Required for Maintaining Glial Homeostasis in Developing and Adult Fly Brains
Felice Frey, Jawdat Sandakly, Mirna Ghannam, Caren Doueiry, Fredrik Hugosson, Johannes Berlandi, Joy N. Ismail, Tenzin Gayden, Martin Hasselblatt, Nada Jabado, Margret Shirinian
eNeuro 8 April 2022, 9 (2) ENEURO.0418-21.2022; DOI: 10.1523/ENEURO.0418-21.2022
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Keywords

  • 6mA DNA demethylation
  • brain tumor
  • Drosophila brain
  • Drosophila Ten-eleven translocation (Tet)
  • human TET Drosophila model
  • optic chiasm glia

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