Figure 3-1
Supplemental data figure supporting the Figure 3. Effect of metformin on AMPK and p-AMPK in HEK293 cells and DRG neurons. A, Representative blots of AMPK and p-AMPK expression in HEK293 cells, treated with medium or 20 mm metformin. B, Respective quantification of AMPK, p-AMPK expressions, and p-AMPK/AMPK ratio (n = 6/condition). C, Representative blots of AMPK and p-AMPK expression in DRG neurons, treated with control medium or 20 mM metformin. D, Respective quantification of AMPK, p-AMPK expressions, and p-AMPK/AMPK ratio (n = 6/condition). Values were normalized to tubulin expression. Data are represented as a box plot with whiskers extending from the 10th to the 90th percentile; each dot represents one sample. Statistical analysis was performed using an unpaired t test with Welch’s correction (Extended Data Table 1-2). *p < 0.05, **p < 0.01, ***p < 0.001. Detailed references for the resources used are presented in Extended Data Table 1-1. Download Figure 3-1, EPS file.