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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Quantification of Neurite Degeneration with Enhanced Accuracy and Efficiency in an In Vitro Model of Parkinson’s Disease

Rachel T. Clements, Lauren E. Fuller, Kyle R. Kraemer, Samantha A. Radomski, Sarah Hunter-Chang, Wesley C. Hall, Alborz A. Kalantar and Bradley R. Kraemer
eNeuro 24 February 2022, 9 (2) ENEURO.0327-21.2022; DOI: https://doi.org/10.1523/ENEURO.0327-21.2022
Rachel T. Clements
1Department of Biological Sciences, Eastern Kentucky University, Richmond, KY 40475
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Lauren E. Fuller
1Department of Biological Sciences, Eastern Kentucky University, Richmond, KY 40475
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Kyle R. Kraemer
2Department of Psychology, Birmingham-Southern College, Birmingham, AL 35254
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Samantha A. Radomski
1Department of Biological Sciences, Eastern Kentucky University, Richmond, KY 40475
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Sarah Hunter-Chang
3Neuroscience Graduate Program, University of Virginia, Charlottesville, VA 22903
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Wesley C. Hall
1Department of Biological Sciences, Eastern Kentucky University, Richmond, KY 40475
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Alborz A. Kalantar
1Department of Biological Sciences, Eastern Kentucky University, Richmond, KY 40475
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Bradley R. Kraemer
1Department of Biological Sciences, Eastern Kentucky University, Richmond, KY 40475
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0327-21.2022
PubMed 
35210286
Published By 
Society for Neuroscience
History 
  • Received July 23, 2021
  • Revision received January 21, 2022
  • Accepted January 30, 2022
  • Published online February 24, 2022.
Copyright & Usage 
Copyright © 2022 Clements et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Rachel T. Clements1,*,
  2. Lauren E. Fuller1,*,
  3. Kyle R. Kraemer2,
  4. Samantha A. Radomski1,
  5. Sarah Hunter-Chang3,
  6. Wesley C. Hall1,
  7. Alborz A. Kalantar1 and
  8. Bradley R. Kraemer1
  1. 1Department of Biological Sciences, Eastern Kentucky University, Richmond, KY 40475
  2. 2Department of Psychology, Birmingham-Southern College, Birmingham, AL 35254
  3. 3Neuroscience Graduate Program, University of Virginia, Charlottesville, VA 22903
  1. Correspondence should be addressed to Bradley R. Kraemer at bradley.kraemer{at}eku.edu.
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Author contributions

  1. Author contributions: R.T.C. and B.R.K. designed research; R.T.C., L.E.F., S.A.R., S.H.-C., W.C.H., A.A.K., and B.R.K. performed research; R.T.C., L.E.F., K.R.K., and B.R.K. analyzed data; K.R.K., S.H.-C., and B.R.K. wrote the paper.

  2. ↵* R.T.C. and L.E.F. contributed equally to this work.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by Kentucky Biomedical Infrastructure Network (KBRIN) Grants ULRF-13-1493C-04 and ULRF-13-1493B-01, the National Institute of General Medical Sciences (NIGMS) Grant 8P20GM103436-14, and the Battelle-Eastern Kentucky University Science Scholars Program. We acknowledge the Keck Center for Cellular Imaging for the usage of the Zeiss 980 multiphoton Airyscan microscopy system (PI, AP; NIH-0D025156).

Funding

  • Kentucky Biomedical Research Infrastructure Network

    ULRF-13-1493C-04; ULRF-13-1493B-01
  • HHS | NIH | National Institute of General Medical Sciences (NIGMS)

    8P20GM103436-14
  • Battelle-EKU Science Scholars Program

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  • previous version (February 24, 2022).
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Quantification of Neurite Degeneration with Enhanced Accuracy and Efficiency in an In Vitro Model of Parkinson’s Disease
Rachel T. Clements, Lauren E. Fuller, Kyle R. Kraemer, Samantha A. Radomski, Sarah Hunter-Chang, Wesley C. Hall, Alborz A. Kalantar, Bradley R. Kraemer
eNeuro 24 February 2022, 9 (2) ENEURO.0327-21.2022; DOI: 10.1523/ENEURO.0327-21.2022

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Quantification of Neurite Degeneration with Enhanced Accuracy and Efficiency in an In Vitro Model of Parkinson’s Disease
Rachel T. Clements, Lauren E. Fuller, Kyle R. Kraemer, Samantha A. Radomski, Sarah Hunter-Chang, Wesley C. Hall, Alborz A. Kalantar, Bradley R. Kraemer
eNeuro 24 February 2022, 9 (2) ENEURO.0327-21.2022; DOI: 10.1523/ENEURO.0327-21.2022
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Keywords

  • axonal degeneration
  • degeneration index
  • neurite degeneration
  • neurodegeneration
  • oxidative stress
  • Parkinson’s disease

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