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Research ArticleResearch Article: New Research, Neuronal Excitability

Transcriptomically-Guided Pharmacological Experiments in Neocortical and Hippocampal NPY-Positive GABAergic Interneurons

Sanne Beerens, Jochen Winterer, David Lukacsovich, Csaba Földy and Christian Wozny
eNeuro 18 April 2022, 9 (2) ENEURO.0005-22.2022; DOI: https://doi.org/10.1523/ENEURO.0005-22.2022
Sanne Beerens
1Strathclyde Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, G4 0RE, United Kingdom
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Jochen Winterer
2Laboratory of Neural Connectivity, Brain Research Institute, University of Zürich, 8057 Zürich, Switzerland
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  • ORCID record for Jochen Winterer
David Lukacsovich
2Laboratory of Neural Connectivity, Brain Research Institute, University of Zürich, 8057 Zürich, Switzerland
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Csaba Földy
2Laboratory of Neural Connectivity, Brain Research Institute, University of Zürich, 8057 Zürich, Switzerland
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Christian Wozny
1Strathclyde Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, G4 0RE, United Kingdom
3MSH Medical School Hamburg, Medical University, Institute for Molecular Medicine, 20457 Hamburg, Germany
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    Figure 1.

    Single-cell transcriptomics of NPY-positive neurons in AC and CA3. A, Overview image of a coronal neocortical (left) and a horizontal hippocampal (right) brain slice obtained from an NPY-hrGFP mouse. Scale bar: 1 mm. B, Experimental flowchart starting with electrophysiology, followed by scRNAseq and pharmacological experiments. C, Electrophysiological characterization of AC and CA3 NPY-positive neurons. Note the longer latency to action potential firing in CA3 neurons (see also D3). D1–D4, Active and passive electrophysiological properties of NPY-positive neurons. E, Number of detected genes in AC and CA3 NPY-positive neurons. F–H, Differentially expressed genes between AC and CA3 NPY-positive neurons.

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    Figure 2.

    Differentially expressed genes with p-values < 0.001. A, Genes that are significantly higher expressed in AC compared with CA3 neurons. Genes are displayed in the order of their statistical significance (from lower to higher; Parm1, p = 0.00000069). B, Genes that are significantly higher expressed in CA3 compared with AC neurons. Genes are displayed in the order of their statistical significance (from higher to lower; Alcam, p = 0.0000082).

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    Figure 3.

    Transcriptomically-guided pharmacological experiments. Expression of iGluRs (A) and auxiliary subunits of iGluRs (B). C, Expression of 5HTRs in auditory (AC) and CA3 NPY-positive neurons. D, E, Summary of changes in membrane potential following the application of 5HT and the 5HT2aR antagonist ketanserin.

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eneuro: 9 (2)
eNeuro
Vol. 9, Issue 2
March/April 2022
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Transcriptomically-Guided Pharmacological Experiments in Neocortical and Hippocampal NPY-Positive GABAergic Interneurons
Sanne Beerens, Jochen Winterer, David Lukacsovich, Csaba Földy, Christian Wozny
eNeuro 18 April 2022, 9 (2) ENEURO.0005-22.2022; DOI: 10.1523/ENEURO.0005-22.2022

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Transcriptomically-Guided Pharmacological Experiments in Neocortical and Hippocampal NPY-Positive GABAergic Interneurons
Sanne Beerens, Jochen Winterer, David Lukacsovich, Csaba Földy, Christian Wozny
eNeuro 18 April 2022, 9 (2) ENEURO.0005-22.2022; DOI: 10.1523/ENEURO.0005-22.2022
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Keywords

  • 5HT2a
  • GABAergic interneuron
  • neurogliaform cells
  • NPY
  • PatchSeq
  • Shisa9

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