Article Figures & Data
Figures
- Figure 1.
Single-cell transcriptomics of NPY-positive neurons in AC and CA3. A, Overview image of a coronal neocortical (left) and a horizontal hippocampal (right) brain slice obtained from an NPY-hrGFP mouse. Scale bar: 1 mm. B, Experimental flowchart starting with electrophysiology, followed by scRNAseq and pharmacological experiments. C, Electrophysiological characterization of AC and CA3 NPY-positive neurons. Note the longer latency to action potential firing in CA3 neurons (see also D3). D1–D4, Active and passive electrophysiological properties of NPY-positive neurons. E, Number of detected genes in AC and CA3 NPY-positive neurons. F–H, Differentially expressed genes between AC and CA3 NPY-positive neurons.
- Figure 2.
Differentially expressed genes with p-values < 0.001. A, Genes that are significantly higher expressed in AC compared with CA3 neurons. Genes are displayed in the order of their statistical significance (from lower to higher; Parm1, p = 0.00000069). B, Genes that are significantly higher expressed in CA3 compared with AC neurons. Genes are displayed in the order of their statistical significance (from higher to lower; Alcam, p = 0.0000082).
- Figure 3.
Transcriptomically-guided pharmacological experiments. Expression of iGluRs (A) and auxiliary subunits of iGluRs (B). C, Expression of 5HTRs in auditory (AC) and CA3 NPY-positive neurons. D, E, Summary of changes in membrane potential following the application of 5HT and the 5HT2aR antagonist ketanserin.
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