Extended Data Figure 3-8
Spectral differentiation analysis of discrete L0 calcium events. The difference in ND of responses to unscrambled versus scrambled stimuli is plotted for each session by layer (A), area (B), and layer-area pair (C). Results are similar to the main analysis on ΔF/F0 traces shown in Figure 3. This indicates that the differences we observed in ND are driven by differences in the large-timescale patterns of responses rather than small-timescale spectral differences within the windows, consistent with the sparsity of calcium responses in our dataset. A, We fit an LME model with stimulus category (naturalistic or artificial), layer, and their interaction as fixed effects and found a significant interaction (likelihood ratio test, χ2(2) = 11.481, p = 0.00321). Post hoc one-sided z-tests (adjusted for multiple comparisons): L2/3, z = 3.835, p = 0.000188, Cohen’s d = 0.175, 95% CI [0.0478, ∞); L4, z = –0.154, p = 0.916, Cohen’s d = –0.001, 95% CI [–0.0635, ∞); L5, z = –0.507, p = 0.971, Cohen’s d = –0.0318, 95% CI [–0.0761, ∞). B, We fit an LME model with stimulus category (naturalistic or artificial), area, and their interaction as fixed effects and found a significant interaction (likelihood ratio test, χ2(4) = 15.102, p = 0.00445). Post hoc one-sided z-tests (adjusted for multiple comparisons): V1, z = 0.612, p = 0.793, Cohen’s d = 0.076, 95% CI [–0.0483, ∞); LM, z = –0.136, p = 0.982, Cohen’s d = 0.0270, 95% CI [–0.0665, ∞); AL, z = 2.879, p = 0.00995, Cohen’s d = 0.226, 95% CI [0.0329, ∞); PM, z = –1.929, p > 0.999, Cohen’s d = –0.151, 95% CI [–0.161, ∞); AM, z = 2.318, p = 0.05005, Cohen’s d = 0.0984, 95% CI [–0.0179, ∞). A, B, Asterisks indicate significant post hoc one-sided z-tests in the layer (A) and area (B) interaction LME models (**p < 0.01, ***p < 0.001). Boxes indicate quartiles; whiskers indicate the minimum and maximum of data lying within 1.5 times the interquartile range of the 25% or 75% quartiles; diamonds indicate observations outside this range. C, Mean values are indicated by bars. Download Figure 3-8, TIF file.