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Research ArticleResearch Article: New Research, Integrative Systems

Regional Targeting of Bladder and Urethra Afferents in the Lumbosacral Spinal Cord of Male and Female Rats: A Multiscale Analysis

J. P. Fuller-Jackson, P. B. Osborne and J. R. Keast
eNeuro 12 November 2021, 8 (6) ENEURO.0364-21.2021; DOI: https://doi.org/10.1523/ENEURO.0364-21.2021
J. P. Fuller-Jackson
Department of Anatomy and Physiology, University of Melbourne, Parkville, Victoria 3010, Australia
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P. B. Osborne
Department of Anatomy and Physiology, University of Melbourne, Parkville, Victoria 3010, Australia
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J. R. Keast
Department of Anatomy and Physiology, University of Melbourne, Parkville, Victoria 3010, Australia
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Abstract

Sensorimotor circuits of the lumbosacral spinal cord are required for lower urinary tract (LUT) regulation as well as being engaged in pelvic pain states. To date, no molecular markers have been identified to enable specific visualization of LUT afferents, which are embedded within spinal cord segments that also subserve somatic functions. Moreover, previous studies have not fully investigated the patterning within or across spinal segments, compared afferent innervation of the bladder and urethra, or explored possible structural sex differences in these pathways. We have addressed these questions in adult Sprague Dawley rats, using intramural microinjection of the tract tracer, B subunit of cholera toxin (CTB). Afferent distribution was analyzed within individual sections and 3D reconstructions from sections across four spinal cord segments (L5-S2), and in cleared intact spinal cord viewed with light sheet microscopy. Simultaneous mapping of preganglionic neurons showed their location throughout S1 but restricted to the caudal half of L6. Afferents from both LUT regions extended from L5 to S2, even where preganglionic motor pathways were absent. In L6 and S1, most afferents were associated with the sacral preganglionic nucleus (SPN) and sacral dorsal commissural nucleus (SDCom), with very few in the superficial laminae of the dorsal horn. Spinal innervation patterns by bladder and urethra afferents were remarkably similar, likewise the patterning in male and female rats. In conclusion, microscale to macroscale mapping has identified distinct features of LUT afferent projections to the lumbosacral cord and provided a new anatomic approach for future studies on plasticity, injury responses, and modeling of these pathways.

  • continence
  • micturition
  • parasympathetic
  • preganglionic
  • sacral spinal cord
  • visceral afferent

Footnotes

  • The authors declare no competing financial interests.

  • This work was supported by the Office of the Director, Stimulating Peripheral Activity to Relieve Conditions (SPARC) Program, National Institutes of Health under Award Number OT2OD023872 (to J.R.K. and P.B.O.).

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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eneuro: 8 (6)
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November/December 2021
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Regional Targeting of Bladder and Urethra Afferents in the Lumbosacral Spinal Cord of Male and Female Rats: A Multiscale Analysis
J. P. Fuller-Jackson, P. B. Osborne, J. R. Keast
eNeuro 12 November 2021, 8 (6) ENEURO.0364-21.2021; DOI: 10.1523/ENEURO.0364-21.2021

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Regional Targeting of Bladder and Urethra Afferents in the Lumbosacral Spinal Cord of Male and Female Rats: A Multiscale Analysis
J. P. Fuller-Jackson, P. B. Osborne, J. R. Keast
eNeuro 12 November 2021, 8 (6) ENEURO.0364-21.2021; DOI: 10.1523/ENEURO.0364-21.2021
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Keywords

  • continence
  • micturition
  • parasympathetic
  • preganglionic
  • sacral spinal cord
  • visceral afferent

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