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Research ArticleResearch Article: New Research, Novel Tools and Methods

Off-Target Expression of Cre-Dependent Adeno-Associated Viruses in Wild-Type C57BL/6J Mice

Justin J. Botterill, Abdessattar Khlaifia, Brandon J. Walters, Mark A. Brimble, Helen E. Scharfman and Maithe Arruda-Carvalho
eNeuro 16 November 2021, 8 (6) ENEURO.0363-21.2021; DOI: https://doi.org/10.1523/ENEURO.0363-21.2021
Justin J. Botterill
1Department of Psychology, University of Toronto Scarborough, Toronto, Ontario M1C 1A4, Canada
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Abdessattar Khlaifia
1Department of Psychology, University of Toronto Scarborough, Toronto, Ontario M1C 1A4, Canada
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Brandon J. Walters
2Department of Cell & Systems Biology, University of Toronto Mississauga, Mississauga, Ontario L5L 1C6, Canada
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Mark A. Brimble
3Department of Surgery, St. Jude Children’s Research Hospital, Memphis, Tennnessee 38105
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Helen E. Scharfman
4Center for Dementia Research, The Nathan Kline Institute for Psychiatric Research, Orangeburg, New York 10962
5Departments of Child & Adolescent Psychiatry, Neuroscience & Physiology, and Psychiatry, and New York University Neuroscience Institute, New York University Langone Health, New York, New York 10016
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Maithe Arruda-Carvalho
1Department of Psychology, University of Toronto Scarborough, Toronto, Ontario M1C 1A4, Canada
6Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario M5S 3G5, Canada
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eneuro: 8 (6)
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November/December 2021
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Off-Target Expression of Cre-Dependent Adeno-Associated Viruses in Wild-Type C57BL/6J Mice
Justin J. Botterill, Abdessattar Khlaifia, Brandon J. Walters, Mark A. Brimble, Helen E. Scharfman, Maithe Arruda-Carvalho
eNeuro 16 November 2021, 8 (6) ENEURO.0363-21.2021; DOI: 10.1523/ENEURO.0363-21.2021

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Off-Target Expression of Cre-Dependent Adeno-Associated Viruses in Wild-Type C57BL/6J Mice
Justin J. Botterill, Abdessattar Khlaifia, Brandon J. Walters, Mark A. Brimble, Helen E. Scharfman, Maithe Arruda-Carvalho
eNeuro 16 November 2021, 8 (6) ENEURO.0363-21.2021; DOI: 10.1523/ENEURO.0363-21.2021
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Keywords

  • Immunofluorescence
  • antibody amplification
  • double inverted open reading frame
  • fear conditioning
  • c-Fos
  • Cre/loxP
  • DREADDs

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