Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT

User menu

Search

  • Advanced search
eNeuro

eNeuro

Advanced Search

 

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT
PreviousNext
Research ArticleResearch Article: New Research, Novel Tools and Methods

Off-Target Expression of Cre-Dependent Adeno-Associated Viruses in Wild-Type C57BL/6J Mice

Justin J. Botterill, Abdessattar Khlaifia, Brandon J. Walters, Mark A. Brimble, Helen E. Scharfman and Maithe Arruda-Carvalho
eNeuro 16 November 2021, 8 (6) ENEURO.0363-21.2021; DOI: https://doi.org/10.1523/ENEURO.0363-21.2021
Justin J. Botterill
1Department of Psychology, University of Toronto Scarborough, Toronto, Ontario M1C 1A4, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Justin J. Botterill
Abdessattar Khlaifia
1Department of Psychology, University of Toronto Scarborough, Toronto, Ontario M1C 1A4, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brandon J. Walters
2Department of Cell & Systems Biology, University of Toronto Mississauga, Mississauga, Ontario L5L 1C6, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mark A. Brimble
3Department of Surgery, St. Jude Children’s Research Hospital, Memphis, Tennnessee 38105
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mark A. Brimble
Helen E. Scharfman
4Center for Dementia Research, The Nathan Kline Institute for Psychiatric Research, Orangeburg, New York 10962
5Departments of Child & Adolescent Psychiatry, Neuroscience & Physiology, and Psychiatry, and New York University Neuroscience Institute, New York University Langone Health, New York, New York 10016
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maithe Arruda-Carvalho
1Department of Psychology, University of Toronto Scarborough, Toronto, Ontario M1C 1A4, Canada
6Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario M5S 3G5, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Maithe Arruda-Carvalho
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Information

DOI 
https://doi.org/10.1523/ENEURO.0363-21.2021
PubMed 
34785571
Published By 
Society for Neuroscience
History 
  • Received September 8, 2021
  • Revision received October 26, 2021
  • Accepted October 29, 2021
  • Published online November 16, 2021.
Copyright & Usage 
Copyright © 2021 Botterill et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Justin J. Botterill1,
  2. Abdessattar Khlaifia1,
  3. Brandon J. Walters2,
  4. Mark A. Brimble3,
  5. Helen E. Scharfman4,5 and
  6. Maithe Arruda-Carvalho1,6
  1. 1Department of Psychology, University of Toronto Scarborough, Toronto, Ontario M1C 1A4, Canada
  2. 2Department of Cell & Systems Biology, University of Toronto Mississauga, Mississauga, Ontario L5L 1C6, Canada
  3. 3Department of Surgery, St. Jude Children’s Research Hospital, Memphis, Tennnessee 38105
  4. 4Center for Dementia Research, The Nathan Kline Institute for Psychiatric Research, Orangeburg, New York 10962
  5. 5Departments of Child & Adolescent Psychiatry, Neuroscience & Physiology, and Psychiatry, and New York University Neuroscience Institute, New York University Langone Health, New York, New York 10016
  6. 6Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario M5S 3G5, Canada
  1. Correspondence should be addressed to Maithe Arruda-Carvalho at m.arrudacarvalho{at}utoronto.ca.
View Full Text

Author contributions

  1. Author contributions: J.J.B. and M.A.-C designed research; J.J.B. and A.K. performed research; J.J.B., A.K., B.J.W., M.A.B., and M.A.-C. analyzed data; J.J.B., B.J.W., M.A.B., H.E.S., and M.A.-C wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the Human Frontier Science Program Organization (Grants CDA00009/2018 and RGY0072/2019); the Natural Science and Engineering Council of Canada (Grant RGPIN-2017-06344); the Brain and Behavior Research Foundation (formerly NARSAD; Young Investigator Award 26016); the Canadian Institutes of Health Research (CIHR; Grant PJT 399790); the SickKids Foundation and CIHR/Institute of Human Development, Child and Youth Health (Grant NI19-1132R) to M.A.-C.

Funding

  • Human Frontier Science Program (HFSP)

    CDA00009/2018; RGY0072/2019
  • Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (NSERC)

    RGPIN-2017-06344
  • Brain and Behavior Research Foundation (Brain & Behavior Research Foundation)

    NARSAD Young Investigator 26016
  • Gouvernement du Canada | Canadian Institutes of Health Research (CIHR)

    PJT 399790
  • Sick Kids Foundation

    NI19-1132R

Other Version

  • previous version (November 16, 2021).
  • You are viewing the most recent version of this article.

Online Impact

 

Article usage

Select a custom date range for the past year
E.g., 2022-06-22
to
E.g., 2022-06-22

Article usage: November 2021 to May 2022

AbstractFullPdf
Nov 20212012550659
Dec 20215761337254
Total 202125881887913
Jan 202291612149
Feb 202237333155
Mar 202225514177
Apr 20223121284
May 2022616556
Total 20221901836621
Total277837231534
Back to top

In this issue

eneuro: 8 (6)
eNeuro
Vol. 8, Issue 6
November/December 2021
  • Table of Contents
  • Index by author
  • Ed Board (PDF)
Email

Thank you for sharing this eNeuro article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Off-Target Expression of Cre-Dependent Adeno-Associated Viruses in Wild-Type C57BL/6J Mice
(Your Name) has forwarded a page to you from eNeuro
(Your Name) thought you would be interested in this article in eNeuro.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Off-Target Expression of Cre-Dependent Adeno-Associated Viruses in Wild-Type C57BL/6J Mice
Justin J. Botterill, Abdessattar Khlaifia, Brandon J. Walters, Mark A. Brimble, Helen E. Scharfman, Maithe Arruda-Carvalho
eNeuro 16 November 2021, 8 (6) ENEURO.0363-21.2021; DOI: 10.1523/ENEURO.0363-21.2021

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Share
Off-Target Expression of Cre-Dependent Adeno-Associated Viruses in Wild-Type C57BL/6J Mice
Justin J. Botterill, Abdessattar Khlaifia, Brandon J. Walters, Mark A. Brimble, Helen E. Scharfman, Maithe Arruda-Carvalho
eNeuro 16 November 2021, 8 (6) ENEURO.0363-21.2021; DOI: 10.1523/ENEURO.0363-21.2021
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
    • Synthesis
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • Immunofluorescence
  • antibody amplification
  • double inverted open reading frame
  • fear conditioning
  • c-Fos
  • Cre/loxP
  • DREADDs

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

Research Article: New Research

  • Synaptic Integration in CA1 Pyramidal Neurons Is Intact despite Deficits in GABAergic Transmission in the Scn1a Haploinsufficiency Mouse Model of Dravet Syndrome
  • Glutamate Transporters EAAT2 and EAAT5 Differentially Shape Synaptic Transmission from Rod Bipolar Cell Terminals
  • Bisphenol A Exposure Induces Sensory Processing Deficits in Larval Zebrafish during Neurodevelopment
Show more Research Article: New Research

Novel Tools and Methods

  • Comparing surrogates to evaluate precisely timed higher-order spike correlations
  • The Listening Zone of Human Electrocorticographic Field Potential Recordings
  • SMART: An Open-Source Extension of WholeBrain for Intact Mouse Brain Registration and Segmentation
Show more Novel Tools and Methods

Subjects

  • Novel Tools and Methods

  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Latest Articles
  • Issue Archive
  • Blog
  • Browse by Topic

Information

  • For Authors
  • For the Media

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
  • Feedback
(eNeuro logo)
(SfN logo)

Copyright © 2022 by the Society for Neuroscience.
eNeuro eISSN: 2373-2822

The ideas and opinions expressed in eNeuro do not necessarily reflect those of SfN or the eNeuro Editorial Board. Publication of an advertisement or other product mention in eNeuro should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in eNeuro.