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Research ArticleResearch Article: New Research, Disorders of the Nervous System

Knock-Down of Heterogeneous Nuclear Ribonucleoprotein A1 Results in Neurite Damage, Altered Stress Granule Biology, and Cellular Toxicity in Differentiated Neuronal Cells

Amber Anees, Hannah E. Salapa, Patricia A. Thibault, Catherine Hutchinson, S. Austin Hammond and Michael C. Levin
eNeuro 25 October 2021, 8 (6) ENEURO.0350-21.2021; https://doi.org/10.1523/ENEURO.0350-21.2021
Amber Anees
1Office of Saskatchewan Multiple Sclerosis Clinical Research Chair, CMSNRC (Cameco MS Neuroscience Research Center), College of Medicine, University of Saskatchewan, Saskatoon, SK, S7K 0M7, Canada
3Department of Health Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A2, Canada
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Hannah E. Salapa
1Office of Saskatchewan Multiple Sclerosis Clinical Research Chair, CMSNRC (Cameco MS Neuroscience Research Center), College of Medicine, University of Saskatchewan, Saskatoon, SK, S7K 0M7, Canada
2Department of Medicine, Neurology Division, University of Saskatchewan, Saskatoon, SK, S7N 5A2, Canada
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Patricia A. Thibault
1Office of Saskatchewan Multiple Sclerosis Clinical Research Chair, CMSNRC (Cameco MS Neuroscience Research Center), College of Medicine, University of Saskatchewan, Saskatoon, SK, S7K 0M7, Canada
2Department of Medicine, Neurology Division, University of Saskatchewan, Saskatoon, SK, S7N 5A2, Canada
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Catherine Hutchinson
1Office of Saskatchewan Multiple Sclerosis Clinical Research Chair, CMSNRC (Cameco MS Neuroscience Research Center), College of Medicine, University of Saskatchewan, Saskatoon, SK, S7K 0M7, Canada
2Department of Medicine, Neurology Division, University of Saskatchewan, Saskatoon, SK, S7N 5A2, Canada
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S. Austin Hammond
4Next-Generation Sequencing Facility, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada
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Michael C. Levin
1Office of Saskatchewan Multiple Sclerosis Clinical Research Chair, CMSNRC (Cameco MS Neuroscience Research Center), College of Medicine, University of Saskatchewan, Saskatoon, SK, S7K 0M7, Canada
2Department of Medicine, Neurology Division, University of Saskatchewan, Saskatoon, SK, S7N 5A2, Canada
5Department of Anatomy, Physiology and Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, SK, S7N 5A2, Canada
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0350-21.2021
PubMed 
34697074
Published By 
Society for Neuroscience
History 
  • Received August 23, 2021
  • Revision received September 29, 2021
  • Accepted October 18, 2021
  • Published online October 25, 2021.
Copyright & Usage 
Copyright © 2021 Anees et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Amber Anees1,3,
  2. Hannah E. Salapa1,2,
  3. Patricia A. Thibault1,2,
  4. Catherine Hutchinson1,2,
  5. S. Austin Hammond4 and
  6. Michael C. Levin1,2,5
  1. 1Office of Saskatchewan Multiple Sclerosis Clinical Research Chair, CMSNRC (Cameco MS Neuroscience Research Center), College of Medicine, University of Saskatchewan, Saskatoon, SK, S7K 0M7, Canada
  2. 2Department of Medicine, Neurology Division, University of Saskatchewan, Saskatoon, SK, S7N 5A2, Canada
  3. 3Department of Health Sciences, University of Saskatchewan, Saskatoon, SK, S7N 5A2, Canada
  4. 4Next-Generation Sequencing Facility, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada
  5. 5Department of Anatomy, Physiology and Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, SK, S7N 5A2, Canada
  1. Correspondence should be addressed to Michael C. Levin at michael.levin{at}usask.ca.
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Author contributions

  1. Author contributions: A.A., H.E.S., P.A.T., and M.C.L. designed research; A.A. and C.H. performed research; S.A.H. contributed unpublished reagents/analytic tools; A.A. and H.E.S. analyzed data; A.A., H.E.S., and M.C.L. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the Office of the Saskatchewan Clinical Research Chair, Medicine Department, Neurology Division, University of Saskatchewan, and the Saskatchewan Health Region Foundation, Saskatchewan, Canada. Research described in this article was performed in part by Aren Boulet at the Next-Generation Sequencing Facility, which is supported by the Saskatchewan Cancer Agency and College of Medicine, University of Saskatchewan.

Funding

  • University of Saskatchewan (U of S)

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  • You are viewing the most recent version of this article.
  • previous version (October 25, 2021).

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AbstractFullPdf
Oct 2021298097
Nov 2021739611160
Dec 20218245277
Total 202111191063334
Jan 20225523135
Feb 20221122118
Mar 20221511626
Apr 2022119721
May 2022611320
Jun 2022413127
Jul 202269612
Aug 202238015
Sep 202236426
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Nov 2022179115
Dec 202235821
Total 20221441384258
Jan 202337923
Feb 20230729
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May 202307418
Jun 202349620
Jul 2023110017
Aug 202359627
Sep 20231010019
Oct 2023911425
Nov 2023819541
Dec 20231610822
Total 2023601216250
Jan 20241710718
Feb 2024108215
Mar 20241112137
Apr 20241234133
May 20241211036
Jun 2024410115
Jul 2024110227
Aug 20247969
Sep 2024310526
Oct 202449912
Nov 20241310615
Dec 2024179234
Total 20241111462277
Jan 20251210331
Feb 2025611013
Mar 2025129923
Apr 2025611233
May 2025611021
Jun 20251210331
Jul 20251211736
Aug 20251811438
Sep 20251615120
Oct 20251210921
Nov 20251512732
Dec 2025811544
Total 20251351370343
Jan 2026814023
Feb 20261519527
Mar 20262019053
Apr 2026106142
Total 202653586145
Total162270811607
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Knock-Down of Heterogeneous Nuclear Ribonucleoprotein A1 Results in Neurite Damage, Altered Stress Granule Biology, and Cellular Toxicity in Differentiated Neuronal Cells
Amber Anees, Hannah E. Salapa, Patricia A. Thibault, Catherine Hutchinson, S. Austin Hammond, Michael C. Levin
eNeuro 25 October 2021, 8 (6) ENEURO.0350-21.2021; DOI: 10.1523/ENEURO.0350-21.2021

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Knock-Down of Heterogeneous Nuclear Ribonucleoprotein A1 Results in Neurite Damage, Altered Stress Granule Biology, and Cellular Toxicity in Differentiated Neuronal Cells
Amber Anees, Hannah E. Salapa, Patricia A. Thibault, Catherine Hutchinson, S. Austin Hammond, Michael C. Levin
eNeuro 25 October 2021, 8 (6) ENEURO.0350-21.2021; DOI: 10.1523/ENEURO.0350-21.2021
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Keywords

  • heterogeneous nuclear ribonucleoprotein A1
  • Neuro-2a cell line
  • neurodegenerative disease
  • Neuronal cell damage
  • RNA binding protein
  • small interfering RNA

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