1 | B | Effect of secretory IgM on the % area of IgG deposition at 2 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Sidak’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 21.31 | <0.0001 |
Section −1200 μm | t = 3.528 | 0.0078 |
Section −600 μm | t = 3.513 | 0.0082 |
Section 600 μm | t = 2.957 | 0.0445 |
C | Effect of secretory IgM on the % area of IgG deposition at 10 weeks post-SCI | KO (4), WT (4) mice | Two-way ANOVA Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 1.965 | 0.1657 |
H | Effects of secretory IgM on levels of serum IgG immunoglobulin | KO: BSL (9), 2 weeks (5), 10 weeks (7) mice | One-way ANOVA Holm–Sidak’s multiple comparisons test | One-way ANOVA between WT and KO | F = 6.694 | 0.0002 |
WT: BSL (10), 2 weeks (6), 10 weeks (6) mice | BSL: (KO vs WT) | t = 0.760 | 0.6995 |
2 weeks post-SCI: (KO vs WT) | t = 1.950 | 0.1662 |
10 weeks post-SCI: (KO vs WT) | t = 0.330 | 0.7431 |
2 | B | Effect of secretory IgM on the % area of CD3+ cell infiltration at 2 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Sidak’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 15.93 | 0.0001 |
Section −1200 μm | t = 2.689 | 0.0088 |
Section −600 μm | t = 2.862 | 0.0054 |
Section 0 | t = 2.29 | 0.0248 |
Section 600 μm | t = 2.776 | 0.0069 |
Effect of secretory IgM on the % area of CD3+ cell infiltration at 10 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 0.827 | 0.3659 |
C | Effect of secretory IgM on CD3+ cell counts at 2 weeks post-SCI | KO (5), WT (5) mice | Two-way ANOVA Sidak’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 9.394 | 0.0029 |
Section −1200 μm | t = 2.000 | 0.0486 |
Section −600 μm | t = 2.095 | 0.039 |
Section 0 | t = 2.275 | 0.0253 |
Section 600 μm | t = 2.483 | 0.0149 |
Effect of secretory IgM on CD3+ cell counts at 10 weeks post-SCI | KO (5), WT (5) mice | Two-way ANOVA Sidak’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 7.335 | 0.0081 |
Section −1800 μm | t= 2.351 | 0.021 |
3 | B | Effect of secretory IgM on the % area of CD11b+ cells at 2 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Sidak’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 20.69 | <0.0001 |
Section −1200 μm | t = 2.471 | 0.0157 |
Section 0 | t = 3.031 | 0.0033 |
Section 600 μm | t = 2.800 | 0.0065 |
Effect of secretory IgM on the % area of CD11b+ cell at 10 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Sidak’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype No significant post hoc difference | F = 5.069 | 0.0272 |
C | Effect of secretory IgM on CD11b+ cell counts at 2 weeks post-SCI | KO (5), WT (5) mice | Two-way ANOVA Sidak’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 21.61 | <0.0001 |
Section −1200 μm | t = 2.096 | 0.039 |
Section 600 μm | t = 2.566 | 0.012 |
Effect of secretory IgM on CD11b+ cell counts at 10 weeks post-SCI | KO (5), WT (5) mice | Two-way ANOVA Sidak’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 21.92 | <0.0001 |
Section −2400 μm | t = 2.573 | 0.0117 |
Section −1200 μm | t = 2.421 | 0.0175 |
Section −600 μm | t = 2.647 | 0.0096 |
Section 1200 μm | t = 2.074 | 0.041 |
4 | B | Effect of secretory IgM on % lesional tissue at 2 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 2.681 | 0.1056 |
C | Effect of secretory IgM on % white matter preservation at 2 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 0.199 | 0.6566 |
D | Effect of secretory IgM on % gray matter preservation at 2 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Fisher’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 6.544 | 0.0061 |
Section 0 | t = 2.817 | 0.0062 |
E | Effect of secretory IgM on % lesional tissue at 10 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Fisher’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 22.53 | <0.0001 |
Section −2400 μm | t = 2.041 | 0.0452 |
Section −1200 μm | t = 2.216 | 0.0302 |
Section −600 μm | t = 3.328 | 0.0014 |
4 | F | Effect of secretory IgM on % white matter preservation at 10 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Fisher’s post hoc to compare between KO and WT mice for each distance point Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 33.38 | <0.0001 |
Section −2400 μm | t = 2.579 | 0.0121 |
Section −1200 μm | t = 2.574 | 0.0123 |
Section −60 μm | t = 2.918 | 0.0048 |
Section 1800 μm | t = 2.496 | 0.0151 |
Section 2400 μm | t = 2.331 | 0.0228 |
Section 3000 μm | t = 2.028 | 0.0466 |
G | Effect of secretory IgM on the % gray matter preservation at 10 weeks post-SCI | KO (4), WT (5) mice | Two-way ANOVA Factor 1 (genotype), factor 2 (distance from epileft) | Effect of genotype | F = 0.1437 | 0.7059 |
5 | C | Effect of secretory IgM on step sequence regularity at 4, 8, and 10 weeks post-SCI | | Three-way ANOVA One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI) | Effect of genotype × injury | F = 10.23 | 0.0019 |
4 weeks: KO SCI (10), KO sham (10) WT SCI (7), WT sham (10) mice | | 4 weeks one-way ANOVA | F = 9.625 | 0.0001 |
KO SCI vs KO sham | t = 3.985 | 0.0011 |
KO SCI vs WT SCI | t = 0.427 | 0.9648 |
WT SCI vs WT sham | t = 3.468 | 0.0044 |
8 weeks: KO SCI (10), KO sham (8), WT SCI (7), WT sham (9) mice | | 8 weeks one-way ANOVA | F = 36.14 | <0.0001 |
KO SCI vs KO sham | t = 36.14 | <0.0001 |
KO SCI vs WT SCI | t = 3.675 | 0.0028 |
WT SCI vs WT sham | t = 4.362 | 0.0004 |
10 weeks: KO SCI (7), KO sham (8), WT SCI (6), WT sham (8) mice | | 10 weeks one-way ANOVA | F = 28.85 | <0.0001 |
KO SCI vs KO sham | t = 8.244 | <0.0001 |
KO SCI vs WT SCI | t = 4.415 | 0.0005 |
WT SCI vs WT sham | t = 3.065 | 0.0154 |
D | Effect of secretory IgM on hindlimb swing speed at 4, 8, and 10 weeks post-SCI | | Three-way ANOVA One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI) | Effect of genotype × injury | F = 5.351 | 0.0229 |
4 weeks: KO SCI (10), KO sham (10) WT SCI (7), WT sham (10) mice | | 4 weeks one-way ANOVA | F= 2.606 | 0.0682 |
KO SCI vs KO sham | t = 2.535 | 0.0477 |
KO SCI vs WT SCI | t = 1.015 | 0.682 |
WT SCI vs WT sham | t = 0.979 | 0.7057 |
5 | D | Effect of secretory IgM on hindlimb swing speed at 4, 8, and 10 weeks post-SCI | 8 weeks: KO SCI (10), KO sham (8), WT SCI (7), WT sham (9) mice | | 8 weeks one-way ANOVA | F = 3.214 | 0.0358 |
KO SCI vs KO sham | t = 2.423 | 0.0624 |
KO SCI vs WT SCI | t = 2.372 | 0.0699 |
WT SCI vs WT sham | t = 0.126 | 0.999 |
10 weeks: KO SCI (9), KO sham (8), WT SCI (6), WT sham (8) mice | | 10 weeks one-way ANOVA | F = 5.733 | 0.0127 |
KO SCI vs KO sham | t = 3.123 | 0.0395 |
KO SCI vs WT SCI | t = 2.474 | 0.3076 |
WT SCI vs WT sham | t = 1.04 | 0.668 |
E | Effect of secretory IgM on forelimb swing speed at 4, 8, and 10 weeks post-SCI | | Three-way ANOVA One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI) | Effect of genotype × injury | F = 0.998 | 0.3205 |
4 weeks: KO SCI (10), KO sham (10), WT SCI (7), WT sham (10) mice | | 4 weeks one-way ANOVA | F = 5.912 | 0.0024 |
KO SCI vs KO sham | t = 3.467 | 0.0044 |
KO SCI vs WT SCI | t = 0.139 | 0.9987 |
WT SCI vs WT sham | t = 2.337 | 0.075 |
8 weeks: KO SCI (12), KO sham (8), WT SCI (7), WT sham (9) mice | | 8 weeks one-way ANOVA | F = 9.014 | 0.0002 |
KO SCI vs KO sham | t = 4.364 | 0.0004 |
KO SCI vs WT SCI | t = 0.107 | 0.9994 |
WT SCI vs WT sham | | t = 2.823 | 0.0242 |
10 weeks: KO SCI (9), KO sham (8), WT SCI (6), WT sham (8) mice | | 10 weeks one-way ANOVA | F = 6.61 | 0.0017 |
KO SCI vs KO sham | t = 3.418 | 0.006 |
KO SCI vs WT SCI | t = 0.777 | 0.8278 |
WT SCI vs WT sham | | t = 2.595 | 0.0446 |
F | Effect of secretory IgM on hindlimb print area at 4, 8, and 10 weeks post-SCI | | Three-way ANOVA One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI) | Effect of genotype × injury | F = 1.416 | 0.2371 |
4 weeks: KO SCI (10), KO sham (10), WT SCI (7), WT sham (10) mice | | 4 weeks one-way ANOVA | F = 0.641 | 0.5941 |
KO SCI vs KO sham | t = 0.239 | 0.9934 |
KO SCI vs WT SCI | t = 1.082 | 0.6375 |
WT SCI vs WT sham | t = 1.095 | 0.6291 |
5 | F | Effect of secretory IgM on hindlimb print area at 4, 8, and 10 weeks post-SCI | 8 weeks: KO SCI (12), KO sham (8), WT SCI (7), WT sham (9) mice | | 8 weeks one-way ANOVA | F = 1.1 | 0.3635 |
KO SCI vs KO sham | t = 0.340 | 0.9817 |
KO SCI vs WT SCI | t = 1.393 | 0.4349 |
WT SCI vs WT sham | t = 0.546 | 0.9306 |
10 weeks: KO SCI (9), KO sham (8), WT SCI (6), WT sham (8) mice | | 10 weeks one-way ANOVA | F = 8.787 | 0.0003 |
KO SCI vs KO sham | t = 2.31 | 0.0838 |
KO SCI vs WT SCI | t = 4.399 | 0.0005 |
WT SCI vs WT sham | t = 0.467 | 0.955 |
G | Effect of secretory IgM on forelimb print area at 4, 8, and 10 weeks post-SCI | | Three-way ANOVA One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI) | Effect of genotype × injury | F = 0.676 | 0.4132 |
4 weeks: KO SCI (10), KO sham (10), WT SCI (7), WT sham (10) mice | | 4 weeks one-way ANOVA | F = 0.927 | 0.4387 |
KO SCI vs KO sham | t = 0.808 | 0.8098 |
KO SCI vs WT SCI | t = 0.968 | 0.7129 |
WT SCI vs WT sham | t = 0.526 | 0.937 |
8 weeks: KO SCI (12), KO sham (8), WT SCI (7), WT sham (9) mice | | 8 weeks one-way ANOVA | F = 3.558 | 0.025 |
KO SCI vs KO sham | t = 0.661 | 0.8846 |
KO SCI vs WT SCI | t = 1.111 | 0.6188 |
WT SCI vs WT sham | t = 3.121 | 0.0114 |
10 weeks: KO SCI (9), KO sham (8), WT SCI (6), WT sham (8) mice | | 10 weeks one-way ANOVA | F = 7.978 | 0.0006 |
KO SCI vs KO sham | t = 2.636 | 0.0406 |
KO SCI vs WT SCI | t = 1.231 | 0.5416 |
WT SCI vs WT sham | t = 3.068 | 0.0145 |
H | Effect of secretory IgM on average body speed at 4, 8, and 10 weeks post-SCI | | Three-way ANOVA One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI) | Effect of genotype × injury | F = 0.558 | 0.457 |
4 weeks: KO SCI (10), KO sham (10), WT SCI (7), WT sham (10) mice | | 4 weeks one-way ANOVA | F = 11.75 | <0.0001 |
KO SCI vs KO sham | t = 4.275 | 0.0005 |
KO SCI vs WT SCI | t = 0.757 | 0.8374 |
WT SCI vs WT sham | t = 4.099 | 0.0008 |
5 | H | Effect of secretory IgM on average body speed at 4, 8, and 10 weeks post-SCI | 8 weeks: KO SCI (12), KO sham (8), WT SCI (7), WT sham (9) mice | | 8 weeks one-way ANOVA | F = 13.56 | <0.0001 |
KO SCI vs KO sham | t = 5.539 | <0.0001 |
KO SCI vs WT SCI | t = 1.353 | 0.46 |
WT SCI vs WT sham | t = 2.87 | 0.0215 |
10 weeks: KO SCI (9), KO sham (8), WT SCI (6), WT sham (8) mice | | 10 weeks one-way ANOVA | F = 10.08 | 0.0001 |
KO SCI vs KO sham | t = 3.972 | 0.0014 |
KO SCI vs WT SCI | t = 0.678 | 0.8776 |
WT SCI vs WT sham | t = 3.507 | 0.0048 |