Article Figures & Data
Figures
- Figure 1.
Dose-dependent behavioral effects of AMPH. A, Timeline of AMPH experiments. B, Total distance traveled (left), orofacial stereotypy (middle), and grooming (right) scores are shown after saline (0 mg/kg, n = 5 animals), low-dose (2 mg/kg, n = 5 animals), or high-dose (16 mg/kg, n = 5 animals) AMPH, for 2.5hpi cohort (red) and 24hpi cohort (blue). Dots in bar graphs show measurements per animal. *p < 0.05, **p < 0.01, ***p < 0.001 for comparison among doses; ◊p < 0.05 for comparison between 2.5hpi and 24hpi.
- Figure 2.
Distribution of ChIs in the Str is not affected by AMPH. A, Schematic representations of 10 coronal sections of the Str (from bregma +1.54 to −1.58 mm). Delineations of Str subregions are shown in the right Str (Paxinos and Franklin, 2008): NAc core (orange), NAc shell (magenta), DM Str (green), DL Str (blue). Locations of slices are shown in inset, and locations from bregma are indicated under the slices. B, Stacked bar graphs showing counts of ChIs across Str subregions in 10 coronal hemisections along the rostrocaudal axis after saline (0 mg/kg), low-dose (2 mg/kg), or high-dose (16 mg/kg) AMPH, at 2.5hpi (top) and 24hpi (bottom). C, Total ChI count (top) and ChI density (neurons/mm2; bottom) in each Str subregion are shown, at 2.5hpi (left) and 24hpi (right). Group ns are given in Figure 1. Dots in bar graphs show the average per animal; **p < 0.01 and ***p < 0.001.
- Figure 3.
Morphology of ChIs is not affected by AMPH. A–B, Morphologic characteristics of ChIs in the whole Str (A) and each Str subregion (B) in the same hemisections as shown in the previous figure: area (μm2), perimeter (μm), and circularity after saline (0 mg/kg), low-dose (2 mg/kg), or high-dose (16 mg/kg) AMPH, at 2.5hpi (left) and 24hpi (right). Dots in bar graphs show the average measurements per animal; *p < 0.05, **p < 0.01, ***p < 0.001.
- Figure 4.
Phosphorylation of ribosomal protein S6 (p-rpS6240/244) in ChIs. A, Low-magnification images of ChAT (purple) and p-rpS6240/244 (green) in a Str hemisection (bregma 0.98 mm) with merged images in the middle. Colored rectangles are representative locations of Str subregions and magnified in B–E. Expanded images of the NAc core (B, orange), NAc shell (C, magenta), DM Str (D, green), and DL Str (E, blue) subregions.
- Figure 5.
P-rpS6240/244 intensity in ChIs 2.5hpi and 24hpi AMPH. A, C, Spatial distribution of ChIs with relative p-rpS6240/244 intensity in the same 10 hemisections as shown in the previous morphology figures along the rostrocaudal axis (from bregma +1.54 to −1.58 mm), at 2.5hpi (A) and 24hpi (C). Spatial distribution of ChIs from five animals was superimposed for each injection group: saline (top), low-dose (middle), or high-dose (bottom) AMPH. Each dot represents one ChI and intensity of p-rpS6240/244 is shown on a blue (low level) to red (high level) color scale. B, D, left, Average p-rpS6240/244 intensity in ChIs in the whole Str at 2.5hpi (B) and 24hpi (D) after saline (0 mg/kg), low-dose (2 mg/kg), or high-dose (16 mg/kg) AMPH. Right, Box and whiskers plots showing p-rpS6240/244 intensity in ChIs in each Str subregion at 2.5hpi (B) and 24hpi (D). Dots in bar graphs show the average measurements per animal; *p < 0.05.
- Figure 6.
Comparison of p-rpS6240/244 intensity in ChIs 2.5hpi and 24hpi AMPH. A, B, Comparison of ChI p-rpS6240/244 intensity z-scores compared to the mean intensity in the corresponding saline-injected animals at 2.5hpi (A) and 24hpi (B) of saline (0 mg/kg), low-dose (2 mg/kg), or high-dose (16 mg/kg) AMPH in each Str subregion. Dots in bar graphs show the average measurements per animal; *p < 0.05.
- Figure 7.
Spontaneous ChI firing 24hpi AMPH. A, Whole-cell recordings were made from ChIs in the four Str subregions. B, An example of ChI firing recorded in the DL Str shows the characteristic spontaneous firing (black trace), and the prominent sag in response to hyperpolarizing-current injection (gray trace). C, Spontaneous firing frequencies of ChIs in each Str subregion are shown after saline (0 mg/kg, n = 30 animals), low-dose (2 mg/kg, n = 22 animals), or high-dose (16 mg/kg, n = 28 animals) AMPH at 24hpi. Dots in bar graphs show measurements for individual animals; the numbers of ChIs recorded were 12–13 cells/Str subregion/treatment; ***p < 0.001.
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