Figure 3. Protein expression of STRA6, RALDH1, and RALDH3 in the hippocampus. Representative blots for proteins of interest (A), expression relative to β-actin as a percentage of Y values, L stands for molecular ladder lane. Hippocampal expression of STRA6 (B, C), RALDH1 (D, E), and RALDH3 (G, H). Correlation of protein expression of RALDH1 and LI scores (F). Results shown as bars with individual animal data plotted. Statistical analysis, unpaired two-tailed Student’s t test (B, D, G), one-way ANOVA, with Tukey’s multiple comparisons test (C, E, H), and linear regression (F; all animals, gray line; aged animals, black line); *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Y n = 8 and aged n = 16 (AU n = 8, AI n = 8; B, G, H); Y n = 7 and aged n = 16 (AU n = 7, AI n = 9; D–F). Error bars represent SEM.