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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Generation of an Iba1-EGFP Transgenic Rat for the Study of Microglia in an Outbred Rodent Strain

Jonathan W. VanRyzin, Sheryl E. Arambula, Sydney E. Ashton, Alexa C. Blanchard, Max D. Burzinski, Katherine T. Davis, Serena Edwards, Emily L. Graham, Amanda Holley, Katherine E. Kight, Ashley E. Marquardt, Miguel Perez-Pouchoulen, Lindsay A. Pickett, Erin L. Reinl and Margaret M. McCarthy
eNeuro 20 August 2021, 8 (5) ENEURO.0026-21.2021; https://doi.org/10.1523/ENEURO.0026-21.2021
Jonathan W. VanRyzin
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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  • ORCID record for Jonathan W. VanRyzin
Sheryl E. Arambula
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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  • ORCID record for Sheryl E. Arambula
Sydney E. Ashton
2Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Alexa C. Blanchard
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Max D. Burzinski
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Katherine T. Davis
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Serena Edwards
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Emily L. Graham
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Amanda Holley
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Katherine E. Kight
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Ashley E. Marquardt
2Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Miguel Perez-Pouchoulen
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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  • ORCID record for Miguel Perez-Pouchoulen
Lindsay A. Pickett
2Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Erin L. Reinl
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Margaret M. McCarthy
1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
2Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0026-21.2021
PubMed 
34417284
Published By 
Society for Neuroscience
History 
  • Received January 21, 2021
  • Revision received July 6, 2021
  • Accepted August 11, 2021
  • Published online August 20, 2021.
Copyright & Usage 
Copyright © 2021 VanRyzin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Jonathan W. VanRyzin1,
  2. Sheryl E. Arambula1,
  3. Sydney E. Ashton2,
  4. Alexa C. Blanchard1,
  5. Max D. Burzinski1,
  6. Katherine T. Davis1,
  7. Serena Edwards1,
  8. Emily L. Graham1,
  9. Amanda Holley1,
  10. Katherine E. Kight1,
  11. Ashley E. Marquardt2,
  12. Miguel Perez-Pouchoulen1,
  13. Lindsay A. Pickett2,
  14. Erin L. Reinl1 and
  15. Margaret M. McCarthy1,2
  1. 1Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
  2. 2Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland 21201
  1. Correspondence should be addressed to Margaret M. McCarthy at mmccarthy{at}som.umaryland.edu.
View Full Text

Author contributions

  1. Author contributions: J.W.V. and M.M.M. designed research; J.W.V., S.E. Arambula, S.E. Ashton, A.C.B., M.D.B., K.T.D., S.E., E.L.G., A.H., K.E.K., A.E.M., M.P.-P., L.A.P., and E.L.R. performed research; J.W.V., S.E. Arambula, S.E. Ashton, A.C.B., M.D.B., K.T.D., S.E., E.L.G., A.H., K.E.K., A.E.M., M.P.-P., L.A.P., and E.L.R. analyzed data; J.W.V. and M.M.M. wrote the paper.

View Abstract

Disclosures

  • The authors declare no competing financial interests.

  • This research was funded by the Dean’s Office, University of Maryland School of Medicine; and by Department of Health and Human Services | National Institutes of Health National Institute of Mental Health Grants R01-MH-52716 and R01-MH-091424, National Institute on Drug Abuse Grant R01-DA-039062, and Eunice Kennedy Shriver National Institute of Child Health and Human Development Grant P01-HD-085928 to M.M.M.

Funding

  • HHS | NIH | National Institute on Drug Abuse (NIDA)

    R01 DA039062
  • HHS | NIH | National Institute of Mental Health (NIMH)

    R01 MH052716; R01 MH091424
  • HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)

    P01 HD085928
  • University of Maryland School of Medicine Dean’s Office

Other Version

  • You are viewing the most recent version of this article.
  • previous version (August 20, 2021).

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Article usage: August 2021 to March 2026

AbstractFullPdf
Aug 20213320155
Sep 2021396506207
Oct 202147674110
Nov 20211837589
Dec 20211013034
Total 20218031685595
Jan 20221517519
Feb 20221212541
Mar 20221162939
Apr 20221951533
May 2022313152
Jun 2022218376
Jul 202259838
Aug 202228528
Sep 2022118434
Oct 2022950226
Nov 2022624645
Dec 202214321
Total 2022962816452
Jan 202326615
Feb 202317520
Mar 202316216
Apr 202317517
May 202318126
Jun 2023113164
Jul 202338614
Aug 202326928
Sep 202368830
Oct 20231210619
Nov 202359219
Dec 2023410632
Total 2023391037300
Jan 2024910329
Feb 2024128517
Mar 20241010324
Apr 2024810525
May 20241210824
Jun 202469631
Jul 202429425
Aug 2024910441
Sep 202469926
Oct 2024610528
Nov 202439519
Dec 2024510328
Total 2024881200317
Jan 2025411331
Feb 2025512628
Mar 20251210322
Apr 2025910627
May 2025411524
Jun 2025811123
Jul 20252413348
Aug 20251411733
Sep 20251220829
Oct 20251813239
Nov 20251215233
Dec 20252217538
Total 20251441591375
Jan 20262519147
Feb 20261432922
Mar 202664213
Total 20264556282
Total121588912121
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Generation of an Iba1-EGFP Transgenic Rat for the Study of Microglia in an Outbred Rodent Strain
Jonathan W. VanRyzin, Sheryl E. Arambula, Sydney E. Ashton, Alexa C. Blanchard, Max D. Burzinski, Katherine T. Davis, Serena Edwards, Emily L. Graham, Amanda Holley, Katherine E. Kight, Ashley E. Marquardt, Miguel Perez-Pouchoulen, Lindsay A. Pickett, Erin L. Reinl, Margaret M. McCarthy
eNeuro 20 August 2021, 8 (5) ENEURO.0026-21.2021; DOI: 10.1523/ENEURO.0026-21.2021

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Generation of an Iba1-EGFP Transgenic Rat for the Study of Microglia in an Outbred Rodent Strain
Jonathan W. VanRyzin, Sheryl E. Arambula, Sydney E. Ashton, Alexa C. Blanchard, Max D. Burzinski, Katherine T. Davis, Serena Edwards, Emily L. Graham, Amanda Holley, Katherine E. Kight, Ashley E. Marquardt, Miguel Perez-Pouchoulen, Lindsay A. Pickett, Erin L. Reinl, Margaret M. McCarthy
eNeuro 20 August 2021, 8 (5) ENEURO.0026-21.2021; DOI: 10.1523/ENEURO.0026-21.2021
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Keywords

  • brain
  • EGFP
  • Iba1
  • macrophage
  • microglia
  • transgenic rat

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