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Research ArticleResearch Article: New Research, Development

Tracking Mitochondrial Density and Positioning along a Growing Neuronal Process in Individual C. elegans Neuron Using a Long-Term Growth and Imaging Microfluidic Device

Sudip Mondal, Jyoti Dubey, Anjali Awasthi, Guruprasad Reddy Sure, Amruta Vasudevan and Sandhya P. Koushika
eNeuro 25 May 2021, 8 (4) ENEURO.0360-20.2021; https://doi.org/10.1523/ENEURO.0360-20.2021
Sudip Mondal
1National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka 560065, India
2Department of Mechanical Engineering, The University of Texas at Austin, Austin, Texas 78712
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Jyoti Dubey
1National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka 560065, India
3Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra 400005, India
4Institute for Stem Cell Science and Regenerative Medicine, Bangalore, Karnataka 560065, India
7Manipal Academy of Higher Education, Manipal, Karnataka 576104, India
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Anjali Awasthi
1National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka 560065, India
5Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Rajasthan 333031, India
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Guruprasad Reddy Sure
1National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka 560065, India
6Sastra University, Thirumalaisamudram, Tamil Nadu 613401, India
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Amruta Vasudevan
3Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra 400005, India
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Sandhya P. Koushika
3Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra 400005, India
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0360-20.2021
PubMed 
34035072
Published By 
Society for Neuroscience
History 
  • Received August 16, 2020
  • Revision received April 18, 2021
  • Accepted May 6, 2021
  • Published online May 25, 2021.
Copyright & Usage 
Copyright © 2021 Mondal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Sudip Mondal1,2,
  2. Jyoti Dubey1,3,4,7,
  3. Anjali Awasthi1,5,
  4. Guruprasad Reddy Sure1,6,
  5. Amruta Vasudevan3 and
  6. Sandhya P. Koushika3
  1. 1National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka 560065, India
  2. 2Department of Mechanical Engineering, The University of Texas at Austin, Austin, Texas 78712
  3. 3Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra 400005, India
  4. 4Institute for Stem Cell Science and Regenerative Medicine, Bangalore, Karnataka 560065, India
  5. 5Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Rajasthan 333031, India
  6. 6Sastra University, Thirumalaisamudram, Tamil Nadu 613401, India
  7. 7Manipal Academy of Higher Education, Manipal, Karnataka 576104, India
  1. Correspondence should be addressed to Sudip Mondal at sudipmondal1979{at}gmail.com or Sandhya P. Koushika at spkoushika{at}tifr.res.in.
View Full Text

Author contributions

  1. Author contributions: S.M. and S.P.K. designed research; S.M., J.D., A.A., G.R.S., and A.V. performed research; S.M. and J.D. analyzed data; S.M. and S.P.K. wrote the paper.

Disclosures

  • S.M. and S.P.K. are authors of a pending patent on the microfluidic growth and imaging device (patent application 640/CHE/2011). All other authors declare no competing financial interests.

  • This work was supported by research funding from Department of Biotechnology (S.P.K. and S.M.), Department of Science and Technology (S.M.), Department of Atomic Energy (1303/2/2019/R&D-II/DAE/2079), and PhD fellowship from Council of Scientific and Industrial Research (G.R.S.) and Council of Scientific and Industrial Research – University Grants Commission (J.D.). The spinning disc was supported by Department of Atomic Energy PRISM 12-R&D-IMS-5.02.0202 (to S.P.K.), and the Howard Hughes Medical Institute International Early Career Scientist Grant 55007425 (to S.P.K.). Confocal microscopes were supported by the Department of Science and Technology–Centre for Nanotechnology Grant SR/55/NM-36-2005.

Funding

  • HHMI

    55007425
  • DAE

    DAE-PRISM 12-R&D-IMS5.02.0202

Other Version

  • You are viewing the most recent version of this article.
  • previous version (May 25, 2021).

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May 20212090101
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Jul 2021115437100
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Sep 20212019243
Oct 20211317756
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Total 20218481370640
Jan 20221010672
Feb 2022118959
Mar 2022610755
Apr 2022108227
May 2022711129
Jun 2022610447
Jul 202226430
Aug 202208623
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Total 2022711049435
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May 202309818
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Jul 2023712826
Aug 202379135
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Total 2023591290282
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Mar 20241310326
Apr 20241514432
May 2024210128
Jun 2024610614
Jul 2024413224
Aug 202489224
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Oct 2024310518
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Total 20241001309281
Jan 2025510019
Feb 20253908
Mar 2025310922
Apr 202511997
May 2025712013
Jun 20251210622
Jul 20252406
Total 20254366497
Total112156821735
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Tracking Mitochondrial Density and Positioning along a Growing Neuronal Process in Individual C. elegans Neuron Using a Long-Term Growth and Imaging Microfluidic Device
Sudip Mondal, Jyoti Dubey, Anjali Awasthi, Guruprasad Reddy Sure, Amruta Vasudevan, Sandhya P. Koushika
eNeuro 25 May 2021, 8 (4) ENEURO.0360-20.2021; DOI: 10.1523/ENEURO.0360-20.2021

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Tracking Mitochondrial Density and Positioning along a Growing Neuronal Process in Individual C. elegans Neuron Using a Long-Term Growth and Imaging Microfluidic Device
Sudip Mondal, Jyoti Dubey, Anjali Awasthi, Guruprasad Reddy Sure, Amruta Vasudevan, Sandhya P. Koushika
eNeuro 25 May 2021, 8 (4) ENEURO.0360-20.2021; DOI: 10.1523/ENEURO.0360-20.2021
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Keywords

  • C. elegans
  • touch receptor neurons
  • mitochondria
  • intermitochondrial distances
  • development
  • microfluidic device
  • long-term imaging

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