Figure 4. Brs3 expression in scRNA sequence datasets. A, Percentage of neurons expressing Brs3 in datasets with ≥5 Brs3-positive cells (Extended Data Fig. 4-1, lists of datasets with fewer Brs3-positive cells). The number of detected Brs3-expressing cells is indicated below each bar. An *Total cells, not just neurons. Data are from the following, in order: GSE143818, GSE125065, GSE130597, GSE74672, GSE132355 GSE87544, GSE93374, GSE113576, GSE149344 GSE126836, GSE126836, GSE124061, GSE104323, GSE95315, GSE116470, GSE146983, GSE116470, GSE116470, and GSE82187. B, Dentate gyrus Brs3 expression from http://linnarssonlab.org/dentate/ (Hochgerner et al., 2018). C, t-SNE plot of the Brs3-expressing Arc-ME neurons. D, Heatmap of expression of the top five enriched genes in each cluster. The Brs3 clusters correspond to the original clusters as follows: Hdc/Prph, n01 and n02; Tac2/Pdyn, n20; Pomc/Cartpt, n15 > n14; Crabp1, n26; and Sst, n23 > n12, and n13.