Extended Data Figure 4-1
Optogenetic inactivation of the CFA affected biPress performance but did not disturb 1P or 2P forelimb movement. A, Experimental scheme for photoinhibition of cortico-cortical projections of the 1P-CFA neurons during the pre-1P period. B, Differences between optogenetic light on and off of the latency to 1P performed by contralateral (ContraFL, left) or ipsilateral (IpsiFL, right) to CFA neurons. C, Differences between optogenetic light on and off of the number of 1P by ipsiFL. D, Experimental scheme for the photostimulation of inhibitory neurons in the 1P-CFA (contralateral hemisphere of the CFA to the forelimb performing 1P. An optic cannula was implanted into the 1P-CFA area of VGAT-ChR2 mice. E, Comparisons of the correct rate (left) and differences in correct and incorrect rate between optogenetic light off and on (right) by photostimulation during the 1-2P period. F, Comparisons of the correct rate (left; two-tailed paired t test, p = 0.793 for control; p = 0.11 for VGAT-ChR2) and differences in correct and incorrect rate between optogenetic light off and on (right; two-tailed unpaired t test, p = 0.159, correct; p = 0.679 for overT; p = 0.926 for bothP; p = 0.111 for wrongP) by photostimulation during the pre-1P period (n = 5 littermate control and n = 7 VGAT::ChR2 mice). G, Differences between optogenetic light on and off of the latency to 1P performed by contralateral (left; Mann–Whitney U test, p = 0.03, n = 5 littermate control and n = 7 VGAT-ChR2 mice) or ipsilateral (right; two-tailed unpaired t test, p = 0.644, n = 3 littermate control and n = 7 VGAT-ChR2 mice) to the CFA implanted an optic cannula. H, Differences between optogenetic light on and off of the number of 1P by ipsiFL (two-tailed unpaired t test, p = 0.745, n = 5 litter mate control and n = 7 VGAT-ChR2 mice). All data are represented as mean ± SEM, and individual data are represented as light-colored lines or circles; *p < 0.05. Figure Contributions: Minju Jeong performed the experiments and analyzed the data. Download Figure 4-1, TIF file.