Article Figures & Data
Figures
- Figure 1.
Representative images detailing the automatic COUNTEN image processing sequence for neuronal identification, enumeration, and clustering of HuC/D-immunostained iLM-MP tissue in a single 20× field of view. A single ganglion (in dotted box) is expanded on the right to show steps of neuronal identification, enumeration, and classification into ganglia.
- Figure 2.
COUNTEN provides rapid, objective, and high-concordance identification, enumeration, and clustering of neurons. A, Example of technician-driven “Manual” and “COUNTEN”-driven neuronal identification of the same myenteric ganglion. B, High degree of correlation between COUNTEN-driven enumeration and technician-driven manual enumeration of myenteric neurons per ganglia from the same 100 20× HuC/D-immunostained images shows the conformity of COUNTEN and experienced technician-generated data. C, COUNTEN-generated data of adult male iLM-MP tissue shows no significant difference in mean numbers of HuC/D-immunostained neurons/ganglia, suggesting a similar ganglia size between litter-mate male mice. Data are represented as mean ± SEM. D, Frequency distribution histogram of ganglia size shows an inverse correlation between ganglia size and their relative abundance, as represented by the negative binomial equation. Values on the x-axis are in incremental bin sizes of three neurons per ganglion.
Extended Data
Extended Data 1
The COUNTEN software. Download Extended Data 1, ZIP file.
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