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Research ArticleResearch Article: New Research, Neuronal Excitability

In Vivo Calcium Imaging of CA3 Pyramidal Neuron Populations in Adult Mouse Hippocampus

Gwendolin Schoenfeld, Stefano Carta, Peter Rupprecht, Aslı Ayaz and Fritjof Helmchen
eNeuro 30 July 2021, 8 (4) ENEURO.0023-21.2021; DOI: https://doi.org/10.1523/ENEURO.0023-21.2021
Gwendolin Schoenfeld
1Laboratory of Neural Circuit Dynamics, Brain Research Institute, University of Zurich, Zurich CH-8057, Switzerland
2Neuroscience Center Zurich, University of Zurich, Zurich CH-8057, Switzerland
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Stefano Carta
1Laboratory of Neural Circuit Dynamics, Brain Research Institute, University of Zurich, Zurich CH-8057, Switzerland
2Neuroscience Center Zurich, University of Zurich, Zurich CH-8057, Switzerland
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Peter Rupprecht
1Laboratory of Neural Circuit Dynamics, Brain Research Institute, University of Zurich, Zurich CH-8057, Switzerland
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Aslı Ayaz
1Laboratory of Neural Circuit Dynamics, Brain Research Institute, University of Zurich, Zurich CH-8057, Switzerland
2Neuroscience Center Zurich, University of Zurich, Zurich CH-8057, Switzerland
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Fritjof Helmchen
1Laboratory of Neural Circuit Dynamics, Brain Research Institute, University of Zurich, Zurich CH-8057, Switzerland
2Neuroscience Center Zurich, University of Zurich, Zurich CH-8057, Switzerland
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Abstract

Neuronal population activity in the hippocampal CA3 subfield is implicated in cognitive brain functions such as memory processing and spatial navigation. However, because of its deep location in the brain, the CA3 area has been difficult to target with modern calcium imaging approaches. Here, we achieved chronic two-photon calcium imaging of CA3 pyramidal neurons with the red fluorescent calcium indicator R-CaMP1.07 in anesthetized and awake mice. We characterize CA3 neuronal activity at both the single-cell and population level and assess its stability across multiple imaging days. During both anesthesia and wakefulness, nearly all CA3 pyramidal neurons displayed calcium transients. Most of the calcium transients were consistent with a high incidence of bursts of action potentials (APs), based on calibration measurements using simultaneous juxtacellular recordings and calcium imaging. In awake mice, we found state-dependent differences with striking large and prolonged calcium transients during locomotion. We estimate that trains of >30 APs over 3 s underlie these salient events. Their abundance in particular subsets of neurons was relatively stable across days. At the population level, we found that co-activity within the CA3 network was above chance level and that co-active neuron pairs maintained their correlated activity over days. Our results corroborate the notion of state-dependent spatiotemporal activity patterns in the recurrent network of CA3 and demonstrate that at least some features of population activity, namely co-activity of cell pairs and likelihood to engage in prolonged high activity, are maintained over days.

  • auto-associative network
  • calcium imaging
  • complex spike burst
  • hippocampus CA3
  • juxtacellular
  • locomotion

Footnotes

  • The authors declare no competing financial interests.

  • This work was supported by the Swiss National Science Foundation (SNSF) Projects 31003A_170269, 310030_192617, and Sinergia Project CRSII5-18O316 (to F.H.); the European Research Council (ERC) Advanced Grant BRAINCOMPATH, Project 670757 (to F.H.); a Forschungskredit Postdoc from the University of Zurich (P.R.); and the SNSF Ambizione Grant PZ00P3_161544 (to A.A.).

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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In Vivo Calcium Imaging of CA3 Pyramidal Neuron Populations in Adult Mouse Hippocampus
Gwendolin Schoenfeld, Stefano Carta, Peter Rupprecht, Aslı Ayaz, Fritjof Helmchen
eNeuro 30 July 2021, 8 (4) ENEURO.0023-21.2021; DOI: 10.1523/ENEURO.0023-21.2021

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In Vivo Calcium Imaging of CA3 Pyramidal Neuron Populations in Adult Mouse Hippocampus
Gwendolin Schoenfeld, Stefano Carta, Peter Rupprecht, Aslı Ayaz, Fritjof Helmchen
eNeuro 30 July 2021, 8 (4) ENEURO.0023-21.2021; DOI: 10.1523/ENEURO.0023-21.2021
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Keywords

  • auto-associative network
  • calcium imaging
  • complex spike burst
  • hippocampus CA3
  • juxtacellular
  • locomotion

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