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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Combination of Defined CatWalk Gait Parameters for Predictive Locomotion Recovery in Experimental Spinal Cord Injury Rat Models

Ivanna K. Timotius, Lara Bieler, Sebastien Couillard-Despres, Beatrice Sandner, Daniel Garcia-Ovejero, Florencia Labombarda, Veronica Estrada, Hans W. Müller, Jürgen Winkler, Jochen Klucken, Bjoern Eskofier, Norbert Weidner and Radhika Puttagunta
eNeuro 16 February 2021, 8 (2) ENEURO.0497-20.2021; DOI: https://doi.org/10.1523/ENEURO.0497-20.2021
Ivanna K. Timotius
1Machine Learning and Data Analytics Lab, Department of Computer Science, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen 91052, Germany
2Department of Electronics Engineering, Satya Wacana Christian University, Salatiga 50711, Indonesia
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Lara Bieler
3Institute of Experimental Neuroregeneration, Paracelsus Medical University, Salzburg 5020, Austria
4Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg 5020, Austria
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Sebastien Couillard-Despres
3Institute of Experimental Neuroregeneration, Paracelsus Medical University, Salzburg 5020, Austria
4Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg 5020, Austria
5Austrian Cluster for Tissue Regeneration, Vienna 1200, Austria
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Beatrice Sandner
6Spinal Cord Injury Center, Heidelberg University Hospital, Heidelberg 69118, Germany
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Daniel Garcia-Ovejero
7Laboratory of Neuroinflammation, Hospital Nacional de Paraplejicos, Servicio de Salud de Castilla-La Mancha, Toledo 45071, Spain
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Florencia Labombarda
8Laboratorio de Bioquímica Neuroendocrina, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, C1428 ADN, Argentina
9Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, C1121A6B, Buenos Aires, Argentina
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Veronica Estrada
10Molecular Neurobiology Lab, Department of Neurology, University of Düsseldorf, Düsseldorf 40225, Germany
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Hans W. Müller
10Molecular Neurobiology Lab, Department of Neurology, University of Düsseldorf, Düsseldorf 40225, Germany
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Jürgen Winkler
11Department of Molecular Neurology, University Hospital Erlangen, University of Erlangen-Nürnberg, Erlangen 91054, Germany
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Jochen Klucken
11Department of Molecular Neurology, University Hospital Erlangen, University of Erlangen-Nürnberg, Erlangen 91054, Germany
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Bjoern Eskofier
1Machine Learning and Data Analytics Lab, Department of Computer Science, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen 91052, Germany
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Norbert Weidner
6Spinal Cord Injury Center, Heidelberg University Hospital, Heidelberg 69118, Germany
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Radhika Puttagunta
6Spinal Cord Injury Center, Heidelberg University Hospital, Heidelberg 69118, Germany
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0497-20.2021
PubMed 
33593735
Published By 
Society for Neuroscience
History 
  • Received November 18, 2020
  • Revision received January 18, 2021
  • Accepted January 24, 2021
  • Published online February 16, 2021.
Copyright & Usage 
Copyright © 2021 Timotius et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Ivanna K. Timotius1,2,
  2. Lara Bieler3,4,
  3. Sebastien Couillard-Despres3,4,5,
  4. Beatrice Sandner6,
  5. Daniel Garcia-Ovejero7,
  6. Florencia Labombarda8,9,
  7. Veronica Estrada10,
  8. Hans W. Müller10,
  9. Jürgen Winkler11,
  10. Jochen Klucken11,
  11. Bjoern Eskofier1,
  12. Norbert Weidner6 and
  13. Radhika Puttagunta6
  1. 1Machine Learning and Data Analytics Lab, Department of Computer Science, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen 91052, Germany
  2. 2Department of Electronics Engineering, Satya Wacana Christian University, Salatiga 50711, Indonesia
  3. 3Institute of Experimental Neuroregeneration, Paracelsus Medical University, Salzburg 5020, Austria
  4. 4Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg 5020, Austria
  5. 5Austrian Cluster for Tissue Regeneration, Vienna 1200, Austria
  6. 6Spinal Cord Injury Center, Heidelberg University Hospital, Heidelberg 69118, Germany
  7. 7Laboratory of Neuroinflammation, Hospital Nacional de Paraplejicos, Servicio de Salud de Castilla-La Mancha, Toledo 45071, Spain
  8. 8Laboratorio de Bioquímica Neuroendocrina, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, C1428 ADN, Argentina
  9. 9Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, C1121A6B, Buenos Aires, Argentina
  10. 10Molecular Neurobiology Lab, Department of Neurology, University of Düsseldorf, Düsseldorf 40225, Germany
  11. 11Department of Molecular Neurology, University Hospital Erlangen, University of Erlangen-Nürnberg, Erlangen 91054, Germany
  1. Correspondence should be addressed to Radhika Puttagunta at radhika.puttagunta{at}med.uni-heidelberg.de.
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Author contributions

  1. Author contributions: I.K.T. and R.P. designed research; I.K.T., L.B., B.S., D.G.-O., and V.E. performed research; S.C.-D., F.L., H.W.M., J.W., J.K., B.E., and N.W. contributed unpublished reagents/analytic tools; I.K.T. analyzed data; I.K.T., L.B., S.C.-D., B.S., D.G.-O., F.L., H.W.M., J.W., J.K., B.E., N.W., and R.P. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • I.K.T. was supported by the DAAD Research Grant 57129429. B.E. was supported by the German Research Foundation (DFG), the Heisenberg Professorship Program Grant ES 434/8-1. Work in Study 1 was supported by the Olympia Morata Program at Heidelberg University Medical Faculty for B.S. and R.P. as well as the Interdisciplinary Neurobehavioral Core Grant AZ42-04HV.MED(15)/6/1 in Heidelberg. L.B. and S.C.-D. were supported by the Austrian Agency for International Cooperation in Education and Research (OeAD-GmbH) Grant HR02/2018 and Paracelsus Medical University Research Fund Grants PMU-FFF R-13/05/054-GRA, E-15/22/113-COS, and R-19/04/124-BIG.

Funding

  • DAAD

    57129429
  • DFG

    ES 434/8-1
  • Olympia Morata Programm, Heidelberg University Hospital

  • Interdisciplinary Neurobehavioral Core (INBC), University of Heidelberg

    AZ42-04HV.MED(15)/6/1
  • OeAD-GmbH (OEAD)

    HR02/2018
  • Paracelsus Medical University Research Fund

    R-13/05/054-GRA; E-15/22/113-COS and R-19/04/124-BIG

Other Version

  • previous version (February 16, 2021).
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Combination of Defined CatWalk Gait Parameters for Predictive Locomotion Recovery in Experimental Spinal Cord Injury Rat Models
Ivanna K. Timotius, Lara Bieler, Sebastien Couillard-Despres, Beatrice Sandner, Daniel Garcia-Ovejero, Florencia Labombarda, Veronica Estrada, Hans W. Müller, Jürgen Winkler, Jochen Klucken, Bjoern Eskofier, Norbert Weidner, Radhika Puttagunta
eNeuro 16 February 2021, 8 (2) ENEURO.0497-20.2021; DOI: 10.1523/ENEURO.0497-20.2021

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Combination of Defined CatWalk Gait Parameters for Predictive Locomotion Recovery in Experimental Spinal Cord Injury Rat Models
Ivanna K. Timotius, Lara Bieler, Sebastien Couillard-Despres, Beatrice Sandner, Daniel Garcia-Ovejero, Florencia Labombarda, Veronica Estrada, Hans W. Müller, Jürgen Winkler, Jochen Klucken, Bjoern Eskofier, Norbert Weidner, Radhika Puttagunta
eNeuro 16 February 2021, 8 (2) ENEURO.0497-20.2021; DOI: 10.1523/ENEURO.0497-20.2021
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Keywords

  • CatWalk
  • gait parameter
  • linear discriminant analysis
  • locomotion recovery
  • preclinical development
  • spinal cord injury

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