Article Figures & Data
Figures
- Figure 1.
Ablation of striatal Rgs7 in mice results in an antidepressant-like phenotype. A, Representative Western blottings and graphs of densitometry values for Rgs7 levels in the striatum of WT and Rgs7 sKO mice (n = 4/genotype). B, Scheme of behavioral tests. WT and Rgs7 sKO mice were tested in (C) marble burying (MB), (D) elevated plus maze (EPM), (E) tail suspension test (TST), and (F) forced swim test (FST) (n = 10/genotype). Data shown as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
- Figure 2.
Elimination of Rgs9 does not influence behavior in acute stress procedures. Rgs9 knock-out mice were tested in (A) MB, (B) EPM, (C) TST, and (D) FST (n = 9–11 genotype). Data shown as mean ± SEM; *p < 0.05.
- Figure 3.
Elimination of striatal Rgs7 in mice does not affect cocaine-induced locomotion or CPP. A, Total distance traveled for mice injected with saline or cocaine (15 mg/kg). B, Total distance traveled for mice injected daily with cocaine for 5 d (n = 12 mice/genotype). C, Timeline for CPP. D, Effects of cocaine-induced CPP at doses of 4 and 10 mg/kg (n = 6–11/genotype). Place preference scores are calculated as the difference between time spent in the drug-paired side during postconditioning versus preconditioning tests. Data shown as mean ± SEM.
- Figure 4.
Ablation of striatal Rgs7 in mice display resiliency to stress-induced reinstatement. A, Timeline for reinstatement. B, Time course for extinction of cocaine CPP (n = 5–8 mice/genotype). C, Cocaine reinstatement of extinguished cocaine-induced CPP (n = 5–8 mice/genotype). D, Time course for extinction of cocaine CPP (n = 6–8 mice/genotype). E, Force swim test reinstatement of extinguished cocaine-induced CPP. Data shown as mean ± SEM; *p < 0.05, ***p < 0.001.
- Figure 5.
Proteomic analysis from conditional Rgs7 knock-out mice. Volcano plot showing the protein level fold change relative to significance between WT and Rgs7 sKO mice in the (A) dorsal and (B) ventral striatum. Significantly upregulated proteins are in blue (p < 0.05), significantly downregulated proteins are in red (p < 0.05), and all other proteins are in black (n = 5/genotype). Comparison of fold differences for all quantified proteins found in Extended Data Figure 5-1. C, Panther analysis of statistically overrepresented biological processes in the ventral and dorsal striatum of Rgs7 sKO mice. Dotted line indicates Bonferroni corrected p = 0.05. Shown as a rank ordered list of most significant general biological processes.
Extended Data
Extended Data Figure 5-1
Fold differences between WT and Rgs7 sKO mice for the proteomic analysis. Table listing all quantified proteins between WT and Rgs7 sKO mice in the dorsal and ventral striatum. Columns are arranged left-to-right as protein accession number, gene name, fold change (KO vs WT), Log2 of fold change, Student’s t test, -Log10 of t test, and protein description. Log2 fold change and -Log10 t test are the values graphed in the volcano plots of Figure 5A,B. Download Figure 5-1, XLSX file.
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