3D-Printed Capacitive Sensor Objects for Object Recognition Assays

CapTouch overview. A, Diagram of basic CapTouch system setup with Bare Conductive capacitive sensing board and breadboard. B, Basic novel object recognition workflow.

CapTouch 1.0 NOR results and validation. A, Diagram of CapTouch 1.0 objects spiral (right) and sphere (left). B, Object placement during NOR experiment. C, Cross-sectional view and diagram of wire attachment to the base. D, Percent investigation between the spiral and sphere object during the object preference test (±SEM). E, Percent investigation between the novel and familiar object with a 10-min delay during the NOR experiment. Two mice were excluded from the novel object recognition results because of CapTouch sensing malfunction (±SEM). F, Percent investigation between the novel and familiar object with a 24-h delay during the NOR experiment (±SEM). G, Discrimination index comparing investigation between the novel and familiar objects for the 10-min delay (p = 0.0013 relative to chance) and the 24-h delay (p = 0.0165 relative to chance) NOR experiments. H, Correlation of object investigation duration (seconds) during the object preference test and both the familiarization and test days of the NOR test between capacitive touch sensing and manual scoring (R² = 0.9162, p < 0.001; see Extended Data Figure 2-1 for further validation with an additional manual scorer). I, 30-s example of capacitive touch triggering compared with manual scoring. For additional validation analysis, see Extended Data Figure 2-2.