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PreviousNext
Research ArticleResearch Article: New Research, Sensory and Motor Systems

TLR4 Signaling Selectively and Directly Promotes CGRP Release from Vagal Afferents in the Mouse

Lin Jia, Syann Lee, Jessica A. Tierney, Joel K. Elmquist, Michael D. Burton and Laurent Gautron
eNeuro 14 December 2020, 8 (1) ENEURO.0254-20.2020; DOI: https://doi.org/10.1523/ENEURO.0254-20.2020
Lin Jia
1Center for Hypothalamic Research and Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, TX 75390
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Syann Lee
1Center for Hypothalamic Research and Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, TX 75390
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Jessica A. Tierney
2Neuroimmunology and Behavior Laboratory, Department of Neuroscience, School of Behavioral and Brain Sciences, and Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX 75081
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Joel K. Elmquist
1Center for Hypothalamic Research and Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, TX 75390
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Michael D. Burton
2Neuroimmunology and Behavior Laboratory, Department of Neuroscience, School of Behavioral and Brain Sciences, and Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX 75081
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Laurent Gautron
1Center for Hypothalamic Research and Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, TX 75390
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Abstract

There has been a long-standing debate regarding the role of peripheral afferents in mediating rapid-onset anorexia among other responses elicited by peripheral inflammatory insults. Thus, the current study assessed the sufficiency of peripheral afferents expressing toll-like receptor 4 (TLR4) to the initiation of the anorexia caused by peripheral bacterial lipopolysaccharide (LPS). We generated a Tlr4 null (Tlr4LoxTB) mouse in which Tlr4 expression is globally disrupted by a loxP-flanked transcription blocking (TB) cassette. This novel mouse model allowed us to restore the endogenous TLR4 expression in specific cell types. Using Zp3-Cre and Nav1.8-Cre mice, we produced mice that express TLR4 in all cells (Tlr4LoxTB X Zp3-Cre) and in peripheral afferents (Tlr4LoxTB X Nav1.8-Cre), respectively. We validated the Tlr4LoxTB mice, which were phenotypically identical to previously reported global TLR4 knock-out mice. Contrary to our expectations, the administration of LPS did not cause rapid-onset anorexia in mice with Nav1.8-restricted TLR4. The later result prompted us to identify Tlr4-expressing vagal afferents using in situ hybridization (ISH). In vivo, we found that Tlr4 mRNA was primarily enriched in vagal Nav1.8 afferents located in the jugular ganglion that co-expressed calcitonin gene-related peptide (CGRP). In vitro, the application of LPS to cultured Nav1.8-restricted TLR4 afferents was sufficient to stimulate the release of CGRP. In summary, we demonstrated using a new mouse model that vagally-expressed TLR4 is selectively involved in stimulating the release of CGRP but not in causing anorexia.

  • Cre-loxP
  • innate immunity
  • neuropeptide
  • vagus nerve

Significance Statement

Using a new transgenic mouse model, our data establish that toll-like receptor 4 (TLR4) is both sufficient and required for the release of calcitonin gene-related peptide (CGRP) from a subset of vagal afferents. This finding may be relevant to the understanding of how bacterial infections modulate nerves.

Introduction

Toll-like receptor 4 (TLR4) is the main membrane receptor for lipopolysaccharides (LPS), which are endotoxins derived from the outer membrane of Gram-negative bacteria (Poltorak et al., 1998a,b, 2000; Chow et al., 1999). Hence, the immunologic effects of LPS are blunted in TLR4-deficient mice (Hoshino et al., 1999). That said, other membrane proteins, including the myeloid differentiation factor 2 (Park et al., 2009) and TRP channels (Meseguer et al., 2014; Alpizar et al., 2017) also contribute to LPS signaling. Upon exposure to LPS, anorexia ensues (Kent et al., 1992; Porter et al., 1998a) as the direct result of the actions of proinflammatory molecules on the CNS (Layé et al., 1994; Grossberg et al., 2010; Daniel et al., 2016; Essner et al., 2017). While the anorexic effects of LPS occur within minutes (Liu et al., 2016), LPS does not cross the blood-brain barrier (Banks and Robinson, 2010), suggesting that a peripheral neural mechanism involving vagal afferents may contribute to LPS-induced anorexia. This is because the peripheral endings of vagal afferents are outside the blood-brain barrier and directly accessible to bacterial products located in vagally-innervated tissues. Experiments conducted in deafferented animals are in agreement with this view (Bluthé et al., 1994; Bret-Dibat et al., 1995; Layé et al., 1995; Konsman et al., 2000). However, it was never clear whether LPS could directly act on peripheral neurons until TLR4 was found to be expressed in peripheral afferents, including those in the vagus nerve (Hua et al., 1996; Hosoi et al., 2005; Ochoa-Cortes et al., 2010; de Lartigue et al., 2011; Diogenes et al., 2011; Due et al., 2012; Kunda et al., 2014; Helley et al., 2015; Li et al., 2015; Boonen et al., 2018). Moreover, the ability of isolated sensory neurons to directly and rapidly respond to LPS has been reported in different species using electrophysiology and calcium signaling (Riley et al., 2013; Meseguer et al., 2014). The in vivo administration of LPS has also been reported to produce changes in the neuropeptide expression and firing rate of vagal afferents (Huang and Lai, 2003; Liu et al., 2007). Thus, it is plausible that this mechanism allows peripheral sensory neurons to detect infections in a rapid manner (Clatworthy and Grose, 1999; Chiu et al., 2013; Soldano et al., 2016). Finally, it must be noted that within the brain, TLR4 is expressed in endothelial and glial cells, but not in neurons (Laflamme and Rivest, 2001; Chakravarty and Herkenham, 2005; Kigerl et al., 2007). The latter observation suggests that peripheral afferents are likely the only neurons in the body able to sense changing levels of TLR4 activators in a direct manner.

Other findings contradict the view that TLR4 is present and functional in vagal afferents. Another RNA sequencing study of vagal afferents failed to detect any vagal afferents with significant Tlr4 mRNA expression (Wang et al., 2017). This is without mentioning that several laboratories have demonstrated that interrupting vagal afferents does not prevent the host of physiological responses to peripheral LPS, including anorexia (Bret-Dibat et al., 1997; Schwartz et al., 1997; Hansen et al., 2000; Martin et al., 2000; Wieczorek et al., 2005). Considering that vagal afferents are exquisitely sensitive to a wide range of proinflammatory molecules (Ek et al., 1998; Yu et al., 2005; Feldman-Goriachnik et al., 2015), it remains possible that the aforementioned cellular and physiological effects of LPS are not directly mediated by TLR4 signaling in vagal afferents themselves. Given the above uncertainties in the field, the current study aimed to reassess the sufficiency of vagal afferents TLR4 to select LPS-induced responses using a newly generated transgenic mouse model.

Materials and Methods

Mice

Mice were all housed in a barrier facility in a light-controlled and temperature-controlled environment [zeitgeber (ZT)0 = 6 A.M.] with ad libitum access to standard chow, unless specified otherwise (Harlan Teklad TD.2016 Global). Experimental mice were males, whereas females were only used for breeding purposes. Single housing was required during the course of food intake studies. Otherwise, animals were group housed at all time. All the procedures in this study were approved by our Institutional Animal Care and Use Committees and in accordance with the Society’s Policies on the Use of Animals and Humans in Neuroscience Research.

Wild-type (WT) C57Bl/6 mice

A total of six male mice, between six and eight weeks of age, on a pure C57Bl/6 background were used for chromogenic in situ hybridization (ISH) experiments. These mice were obtained from our Animal Resource Center.

Nav1.8-CreChR2-YFP reporter mice

We also generated and genotyped Nav1.8-Cre-ChR2-YFP mice carrying one Cre allele and one ChR2-YFP allele. A total of four males between six and eight weeks of age, were used for chromogenic ISH combined with GFP immunostaining experiments.

Generation of novel Cre-reactivated TLR4-null mice (Tlr4LoxTB)

To generate Tlr4LoxTB mice, BAC clones containing the murine Tlr4 gene derived from the 129/Sv strain were obtained from the Sanger Institute. The BAC DNA clone number bMQ58F22 was electroporated into EL350 bacteria. First, the loxP sequences present in the BAC backbone were removed by homologous recombination as previously described (Lee et al., 2001). Then the validated loxP-flanked transcription blocking (TB) sequences (Berglund et al., 2012) were amplified by PCR using primers 5′-GCCTCATTTAGAAGTGGAATGATAGAAACTCACAGAAATTAATGGGTTCCCAAGATCATGGGTACCCGCGCCTAGTCGACTTCGAATAACTT-3′ and 5′-TGTTAAAAAGAATATCGCAAGAGGAATCCATGGAGCCATGTTAACTCTCCATTCTTCCTGGGTGGCGGCCGCTTAGTTTA-3′. The amplicon was then inserted between exons 2 and 3 of the Tlr4 gene by homologous recombination in EL350 cells (Lee et al., 2001). Kanamycin-resistant clones were selected and recombination was verified by PCR using the following primers: 5′-CTGGACAAACAGTGGCTGGA-3′ and 5′-GTCATAGATGCATGCCAGATACA-3′. Next, the Amp resistance sequence in the EL350 clones with correctly targeted Tlr4 BAC DNA (LoxTB-Tlr4) was replaced with a Zeocin resistance cassette. pZErO−1 vector (Invitrogen) was used to amplify the Zeocin resistance sequence by PCR, and the amplicon was inserted by homologous recombination. Zeocin resistant clones were selected and recombination was verified by PCR. Following this, the pGEM-T easy vector (Promega) was used as a template to generate an amplicon containing two Tlr4 homology arms using primers: 5′-CCATTCAAACTGGAACATAGCCACCTAATTATTTGTCTCTTGTTAGCCAAGTGAAATAGCgcggccgcCCAACGCGTTGGATGCATAGC-3′ and 5′-GTGTGGGTGAAGGTAAGAGTAGCTGTATGCATTACATAGATGTATGAAATTGTCAAAGAGCGGTATTTTCTCCTTACGCATC-3′. The Tlr4LoxTB gene was inserted into the pGEM-T easy vector through homologous recombination and positive clones were verified by PCR using primers: 5′-ACCTGATGCGGTGTGAAATAC-3′ and 5′-AGGAAACAGCTATGACCATGA-3′. Sequencing of the amplicons was performed and enzymatic digestions were conducted to confirm on-target homologous recombination, the sequences of the loxP-flanked TB cassette, and Tlr4 native sequences. The targeting vector, which consisted of the LoxTB-Tlr4 gene flanked by 3.2-kb (left) and 5.2-kb (right) Tlr4 homology arms was prepared using a commercially available kit (QIAGEN), linearized by PvuI enzymatic digestion and electroporated into 129/SvJ ES cells by the transgenic core facility at The University of Texas Southwestern Medical Center. To identify the recombinant clones, genomic DNA was extracted from ES cells as previously described (Berglund et al., 2012) and used for long PCR assays to discriminate between ES clones hosting WT and loxP-modified Tlr4 allele. Correct targeting was further confirmed by a custom multiplex TaqMan quantitative PCR (qPCR) assay. Briefly, extracted DNA was used as template (5–100 ng). Melanocortin 4 receptor (MC4R) was used as an endogenous reference (ID Mm00457483_s1, FAM dye-labeled probe; Applied Biosystems). Custom primers (5′-TCCTAACAGAAAGTGGAAACTTGAG-3′ and 5′-AGGAATCCATGGAGCCATGTTA-3′) and VIC dye-labeled probe (5′-CCCAAGATCATGCAGGAAGAAT-3′) from Biosearch Technologies were multiplexed with the MC4R assay. DNA-based qPCRs were conducted on an Applied Biosystems PRISM 7900HT sequence detection system. Correctly targeted recombinant ES cells were injected into blastocysts of C57Bl/6 mice. Chimeric male mice (F0) were crossed to C57Bl/6 female mice and their pups (F1) were screened for germ line transmission by PCR using the same strategy as for genotyping. F1 pups bearing the Cre-reactivatable Tlr4-null allele (Tlr4LoxTB) were then crossed with zona pellucida 3 (Zp3)-Cre transgenic mice (stock #003651 C57BL/6-Tg(Zp3-cre)93Knw/J) to restore endogenous TLR4 expression in all tissues (Tlr4LoxTB X Zp3-Cre). Mice carrying a WT and/or Cre-mediated recombination allele (Tlr4LoxTB X Zp3-Cre) were genotyped by primer pairs P1 (5′-CTGACTGGTGTGAAGTGGAATATC-3′) and P3 (5′- GTCATAGATGCATGCCAGATACA-3′). Primers P2 (5′-CTGGACAAACAGTGGCTGGA-3′) and P3 were used to screen the Tlr4LoxTB allele. Globally reactivated mice were used for validation studies and feeding tests. The group size is indicated in figure legends.

Tlr4LoxTB X Nav1.8-Cre mice

F1 pups bearing the Cre-reactivated Tlr4LoxTB allele were then crossed with Nav1.8-Cre mice to produce mice with endogenous TLR4 reactivation only in Nav1.8 neurons (Tlr4LoxTB X Nav1.8-Cre). These mice are referred to as Nav1.8-restricted Tlr4 mice. For all studies, breeding was set up to obtain the following groups: control, Tlr4LoxTB, and Nav1.8-restricted Tlr4 mice. Controls consisted of mice expressing one copy of the Nav1.8-Cre allele. Tlr4LoxTB mice expressed two copies of the Tlr4LoxTB allele without Cre. Nav1.8-restricted Tlr4 mice expressed two copies of the Tlr4LoxTB allele and one Cre allele. To validate our ISH probe, we also compared the ganglia from two Tlr4LoxTB mice to that of two globally reactivated mice (Tlr4LoxTB x Zp3-Cre). Mice were maintained on a mixed (C57Bl/6 and 129) genetic background. Male mice entered the experiments at 8–16 weeks of age and were used for feeding and calcitonin gene-related peptide (CGRP) studies. The group size is indicated in figure legends.

LPS preparation and dosage

For animal and culture experiments, we kept 1 mg/ml aliquots of LPS solution (Sigma L2880 Escherichia coli 055:B5) in sterile pyrogen-free 0.9% saline (Sigma) at −20°C. When needed, LPS was diluted 100 times in sterile saline for animal studies or in sterile media for culture studies. In all our feeding studies, LPS was administered at a single dose of 100 μg/kg of body weight (intraperitoneal). This moderate dose is sufficient to induce rapid and transient anorexia in the mouse (Bret-Dibat and Dantzer, 2000; Lawrence et al., 2012; Liu et al., 2016). A dose of 2 mg/kg (intraperitoneal) was used to stimulate Nfkbia expression in the nodose ganglion. This dosage was chosen based on a prior study demonstrating LPS-induced gene expression changes in the nodose ganglion (Huang and Lai, 2003). A dose of 500 ng/ml of LPS was used for nodose organotypic culture studies (Marrone et al., 2017).

LPS-induced inflammatory response in mice

WT, Tlr4LoxTB, and Tlr4LoxTB X Zp3-Cre mice (7–10 weeks old) were treated with LPS (1 or 0.5 mg/kg body weight) by intraperitoneal injection. Blood was collected 1.5 h after injection by tail bleeding. Plasma TNFα concentrations were determined using the MILLIPLEX MAP Mouse Cytokine/Chemokine panel (Millipore).

Genomic DNA isolation

Genomic DNA was isolated from tails of six-week-old mice using REDExtract-N-Amp Tissue PCR kit (Sigma) following the manufacturer’s instructions.

Feeding studies

Individually-housed control, Tlr4 deficient, and reactivated mice were fasted overnight. At ∼9 A.M., fasted mice were weighed and immediately treated with either LPS or sterile saline. At the same time, mice were refed with chow. An experimenter continuously recorded the feeding behavior of each mouse over 60 min following treatment. At the end of the 60-min period, food pellets and body weight were measured. Data were used to calculate the total amount of time spent eating in seconds.

LPS-induced gene expression studies by qPCR

Mice were fasted overnight. The next morning, body weights were measured to determine the injection volume of saline or LPS. Then, saline or LPS was administered at the single dosage of 2 mg/kg, intraperitoneally. One hour postinjection, mice received an overdose of chloral hydrate (500 mg/kg, i.p.) and their nodose-jugular ganglia were rapidly removed and frozen in liquid nitrogen. Total RNAs were extracted using RNA Stat60 (Teltest) according to the manufacturer’s instructions. RNA concentration and quality were determined by NanoDrop 1000 Spectrophotometer (Thermo Scientific). Complementary DNA was synthesized using the High Capacity cDNA kit and was performed at 25°C for 10 min, 37°C for 120 min, and 85°C for 5 min (Applied Biosystems). Primers for Tlr4 (ID: Mm0445274_m1), Nfkbia (ID: Mm00477798_m1), and 18s (ID: Hs99999901_s1) were purchased from Applied Biosystems. The mRNA contents were normalized to 18s mRNA levels. qPCR was performed in an ABI Prism 7900HT sequence detection system (Applied Biosystems) using TaqMan Master Mix (Applied Biosystems) and 10 ng of synthesized cDNA. Samples were run in triplicates. The relative amounts of all mRNAs were calculated using the ΔΔCT assay.

ISH, microscopy, and digital images analysis

Mice received an overdose of chloral hydrate (500 mg/kg, i.p.), before being transcardiacally perfused with 10% formalin (Sigma). The nodose-jugular ganglionic mass was rapidly dissected and kept in formalin for an additional 24 h at 4°C. Fixed ganglia were next incubated overnight in a solution of 20% sucrose in PBS (pH 7.4) at 4°C before being frozen on a bed of dry ice. Series of 14-μm sections were collected on SuperFrost slides using a cryostat. Tissue was stored at −80°C for less than a couple of weeks. The tissue was processed for ISH following the manufacturer’s instructions (Advanced Cell Diagnostics) with only minor adjustments to the pretreatment. Specifically, slides were baked for ∼30 min at 60°C before starting the pretreatment. In addition, the target retrieval solution was heated up to 87°C instead of 99°C. Table 1 summarizes the probes, chromogenic labels, and reagents used in the current study. Tissue sections labeled for Tlr4 alone or Tlr4 combined with Calca (CGRP precursor gene) were counterstained with a hematoxylin solution (GHS132 Sigma). Tissue sections from Nav1.8-CreChR2-YFP mice were not counterstained, but further processed for GFP immunohistochemistry. Briefly, after several PBS washes, the sections were incubated overnight at room temperature in a chicken anti-GFP primary antiserum (Aves Laboratory; GFP-10 120; RRID:AB_10000240; 1:1000) in 3% normal donkey serum with 0.25% Triton X-100 in PBS. On the following day, sections were washed and incubated for 1 h in a biotinylated anti-chicken secondary antibody (catalog #703065155; Jackson ImmunoResearch; 1:1000), followed by Streptavidin Alexa Fluor 488 (Invitrogen, #S11223; 1:1000). All our slides were coverslipped with EcoMount mounting medium (Biocare Medical, LLC EM897L).

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Table 1

List of reagents used for ISH

Bright-field images were captured using a Zeiss microscope (Imager ZI) attached to a digital camera (Axiocam). A camera lucida attached to the microscope was also used to draw tissue sections. Drawings were digitalized and exported to Adobe Illustrator Artwork 15.1. The Zeiss microscope (Imager ZI) was also used for manual counts of the ISH signals for cell profiles positive for Tlr4/H&E as well as Tlr4/Calca. Cell profiles were counted on digital images by an experimenter unaware of our experimental design. Fluorescent digital images were all acquired with the 20× objective of a Zeiss microscope (Imager ZI) or the 63× objective of a Leica TCS SP5 confocal microscope (The University of Texas Southwestern Live Cell Imaging Core). Scanning parameters were adjusted appropriately to improve the signal/background. We collected Z-stacks separated by ∼0.35–0.45 μm in a 512 × 512-pixel format. NIH ImageJ software was used to generate our final TIFF images with combined Z stacks. Adobe Photoshop CS2 software was used to combine digital images into plates with annotations. The contrast and brightness of digital images were uniformly adjusted. Neuronal versus non-neuronal profiles were usually easily identifiable by the size, shape and intensity of the counterstaining of their nuclei. Our counts should be considered estimates rather than absolute counts. Cells that were considered doubly labeled included profiles with accumulation of Tlr4 mRNA (at least one red dot per profile) within the boundaries of one Calca-positive profile. In addition, the circumference of each identified cell profile was assessed using the measurement tools in ImageJ. Images acquired by confocal microscopy were further evaluated for doubly labeled cells using plot of fluorescence intensities. Briefly, we manually traced a line across identified cell profiles using the ImageJ software (NIH, Fiji version). Plot profiles of gray values were generated for each color channel (overlapping red and green). Representative plot profiles were included in our figures. Cells with colocalized fluorescence displayed overlapping red and green plots of fluorescence across the line profile. Cells without staining or minimal colocalization displayed a flat plot profile in at least one channel. Colocalization data were expressed as the mean percentage of identified cell profiles ± SEM, and the absolute numbers of counted profiles were included in the figures.

Organotypic nodose ganglion and CGRP assays

Our protocol was based on previous literature (Gavini et al., 2018). In particular, we chose to culture ganglia for 5–7 d to give the samples time to rebound from the injury caused by the acute dissection. Male mice (five to eight weeks) were deeply anesthetized with isoflurane before being decapitated and the nodose/jugular ganglia were quickly removed and stored in chilled HBSS (Invitrogen) on ice. The isolated ganglia were placed on a 30 mm, 0.4 μm pore size, hydrophilic Millicell culture insert (Millipore Sigma; catalog #PICM03050) and maintained on the insert in DMEM F-12 GlutaMax media (Invitrogen) supplemented with 20% heat-inactivated horse serum (Invitrogen, Life Technologies), and 1× penicillin streptomycin (Invitrogen). Cultures were maintained for 5–7 d with media changes every other day. After an overnight incubation in low serum (2.0%), cultures were stimulated with 500 ng/ml LPS or vehicle for 24 h before supernatant was collected. Supernatants were centrifuged to remove debris and loaded to a CGRP ELISA kit (Cayman Chemical; catalog #589001) and the protocol ran according to manufacturer’s instructions.

Statistical analysis

All of the quantitative data are expressed as the mean value ± SEM. The numbers of mice per group are indicated in each figure. Statistical analysis was performed using GraphPad Prism software, version 6.07. As indicated in the legends, the data were analyzed using Student’s unpaired t test, one-way ANOVA or two-way ANOVA, followed by the post hoc test recommended by the software; p < 0.05 was considered statistically significant. Lower case letters were used to indicate groups found to be significantly different by the post hoc analysis. We also followed the journal’s recommendations in terms of data representations and estimation statistics (Ho et al., 2019). Estimation statistics are included in the legends of our main figures or Extended Data.

Results

Validation of a novel reactivable TLR4 mouse model

We designed and generated a novel Tlr4 null (Tlr4LoxTB) mice whose Tlr4 expression is globally disrupted by a loxP-flanked TB cassette (LoxTB) that was inserted into the coding region of the Tlr4 gene (Fig. 1A). Crossing Tlr4loxTB mice with Zp3-Cre mice produced mice in which expression of endogenous Tlr4 was globally reactivated in all cells (Fig. 1A,B). As a result, the LPS-induced inflammatory response in these mice was identical to WT mice (Fig. 1C,D). Specifically, the administration of LPS produced a not significantly different increase in circulating TNF-α in WT and reactivated animals. However, Tlr4LoxTB mice (without Cre) behaved like global TLR4 knock-out mice and failed to respond to LPS (Fig. 1C,D). In a separate cohort, we tested the anorectic effects of a small dose of LPS (Fig. 1E). As anticipated, LPS suppressed food intake in Zp3-Cre mice, but not in Tlr4LoxTB mice (without Cre). The anorectic effect of LPS (100 μg/kg, i.p.) was fully restored in mice with Tlr4 reactivated in all cells that normally express it (Fig. 1E). This is because the expression of Tlr4 remains driven by its endogenous promoter. These studies demonstrate the validity of our reactivable model.

Figure 1.
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Figure 1.

A, A loxP-flanked transcription-blocking cassette was inserted between exons 2 and 3 of the Tlr4 gene to generate mice lacking TLR4. B, Genotyping analysis of tail genomic DNA from WT, Tlr4LoxTB, and Tlr4LoxTB X Zp3-Cre mice. C, D, Plasma TNFα levels were measured in WT, Tlr4LoxTB and Tlr4LoxTB X Zp3-Cre mice (n = 3–5) 1.5 h after intraperitoneally injection of LPS with the concentration of either 1 mg/kg BW (C) or 0.5 mg/kg BW (D). In C, data were analyzed using one-way ANOVA for genotype (p = 0.0013), followed by Dunnett’s test (WT vs Tlr4LoxTB p = 0.0017). In D, data were analyzed using one-way ANOVA for genotype (p = 0.0004), followed by Dunnett’s test (WT vs Tlr4LoxTB p = 0.0003). For cytokines, letters denote significant differences between columns with p < 0.05, compared with corresponding Tlr4LoxTB mice. E, Graphs of 1-h cumulative food intake in response to saline or LPS (100 μg/kg, i.p.) in control ZP3Cre (white), Tlr4LoxTB (black), and Zp3-Cre-reactivated mice (gray). Groups consisted of n = 5. Data are expressed as mean ± SEM and were analyzed using two-way ANOVA for genotype (p = 0.0116), treatment (p = 0.0002), and interaction (p = 0.001), followed by Sidak’s post hoc test. For each genotype, letters denote significant differences between columns with p < 0.05, compared with corresponding saline-treated mice. Estimation statistics was calculated in LPS-treated mice. Estimation statistics are included in Extended Data Figure 1-1.

Extended Data Figure 1-1

A, B, Estimation statistic corresponding to Figure 1C,D. Mean difference for two comparisons against the shared control WT are shown in the above Cumming estimation plot. The raw data are plotted on the upper axes. On the lower axes, mean differences are plotted as bootstrap sampling distributions. Each mean difference is depicted as a dot. Each 95% confidence interval is indicated by the ends of the vertical error bars. C, Estimation statistic corresponding to Figure 1E. Mean difference for two comparisons against the shared control Zp3-Cre are shown in the above Cumming estimation plot. The raw data are plotted on the upper axes. On the lower axes, mean differences are plotted as bootstrap sampling distributions. Each mean difference is depicted as a dot. Each 95% confidence interval is indicated by the ends of the vertical error bars. Download Figure 1-1, TIF file.

Tlr4-expressing vagal afferents do not initiate LPS-induced anorexia

To test for functional TLR4 signaling in vagal afferents in vivo, we used the previously described Nav1.8-Cre line (Stirling et al., 2005) to generate cohorts of mice that endogenously express Tlr4 only in Nav1.8 neurons. By qPCR, we show that Tlr4 mRNA expression in the nodose-jugular ganglion was 15-fold higher in control mice than in Tlr4LoxTB mice (without Cre; Fig. 2A). In Nav1.8-restricted Tlr4 animals, Tlr4 expression in the nodose-jugular ganglion was ∼8-fold higher than in Tlr4LoxTB mice (Fig. 2A). Tlr4 was selectively re-expressed in Nav1.8 neurons, but not other Tlr4-expressing cell types. For example, Tlr4 was not reactivated in the liver, a tissue with high endogenous expression of Tlr4, but without Nav1.8-Cre cells (Fig. 2B). Lastly, the administration of LPS (2 mg/kg, i.p.) stimulated the expression of Nfkbia, a well-known TLR4 target gene (Quan et al., 1997), in the nodose-jugular ganglion of control animals (Fig. 2C). As anticipated, LPS did not elicit a transcriptional response in Tlr4LoxTB mice (Fig. 2C). LPS also failed to significantly increase Nfkbia expression in the ganglia of Nav1.8-restricted Tlr4 mice (Fig. 2C). This result is consistent with the view that TLR ligands and cytokines are generally poor inducers of the NF-κB pathway in neurons compared with other cell types (Listwak et al., 2013). Overall, our data established the successful re-expression of Tlr4 in vagal afferents.

Figure 2.
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Figure 2.

A, Tlr4 mRNA expression in the nodose-jugular ganglionic mass of control, Tlr4LoxTB, and Tlr4LoxTB crossed with Nav1.8-Cre mice (Nav1.8-restricted TLR4). Our qPCR demonstrated the re-expression of Tlr4 in vagal afferents of reactivated animals. Groups consisted of n = 8–15 mice. B, Restoration of Tlr4 expression was not observed in the liver of reactivated mice, demonstrating the specificity of the Cre-reactivated allele. Groups consisted of n = 3–7 mice. C, Expression of Nfkbia mRNA in saline-treated and LPS-treated mice (2 mg/kg, i.p.) of each respective genotype. Groups consisted of n = 4–7 mice. Data were all obtained by qPCR, and 18S was used as a control gene. Data were analyzed using two-way ANOVA (treatment and genotype factors) followed by Sidak’s post hoc test. Letters denote significant differences between columns with p < 0.05. NG-JG: nodose-jugular ganglia; Nfkbia, NFκB inhibitor α. Estimation statistics are included in Extended Data Figure 2-1.

Extended Data Figure 2-1

A, B, Estimation statistic corresponding to Figure 2A,B. Mean difference for two comparisons against the shared controls are shown in the above Cumming estimation plot. The raw data are plotted on the upper axes. On the lower axes, mean differences are plotted as bootstrap sampling distributions. Each mean difference is depicted as a dot. Each 95% confidence interval is indicated by the ends of the vertical error bars. C, Estimation statistic corresponding to Figure 2C. Estimation statistics was calculated in LPS-treated mice. The mean difference for two comparisons against the shared controls are shown in the above Cumming estimation plot. The raw data are plotted on the upper axes. On the lower axes, mean differences are plotted as bootstrap sampling distributions. Each mean difference is depicted as a dot. Each 95% confidence interval is indicated by the ends of the vertical error bars. Download Figure 2-1, TIF file.

Early LPS-induced anorexia was investigated in control Nav1.8-Cre mice with intact Tlr4 expression. As anticipated, they showed rapid anorexia, starting ∼15 min following LPS injection (Fig. 3A). The total amount of food that LPS-treated mice ate over 1 h was also significantly reduced (Fig. 3B). Weight gain was significantly reduced in LPS-treated mice (Fig. 3C). In contrast, LPS did not elicit anorexia or weight loss in mice that completely lacked TLR4 expression (Fig. 3D–F). In mice expressing TLR4 only in Nav1.8 cells, LPS similarly failed to trigger anorexia or weight loss (Fig. 3G,H). We concluded that TLR4-bearing vagal Nav1.8 neurons do not contribute to the anorectic and cachectic actions of LPS at this particular dose and time point.

Figure 3.
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Figure 3.

A, D, G, Graphs of the cumulative food intake in response to saline (white) or LPS (black; 100 μg/kg, i.p.) in control, Tlr4LoxTB, and Nav1.8-restricted TLR4 littermates. Groups consisted of n = 5–8 mice. Data were analyzed using two-way ANOVA (time and treatment) separately for each genotype, followed by Sidak’s post hoc test. The small letter b indicates points that are different from the saline-treated group. B, E, H, Graph representing food intake or body weight change (C, F, I) over the hour following LPS or saline treatment. Data were analyzed using Student’s unpaired t test. Small letters indicate significant differences between columns. Data are all expressed as the mean values ± SEM over a period of 60 min. Estimation statistics are included in Extended Data Figure 3-1.

Extended Data Figure 3-1

A, B, Estimation statistic corresponding to Figure 3B,C. The mean difference between controls-saline and controls-LPS for food (A) and weight change (B) are shown in the Gardner–Altman estimation plot. Both groups are plotted on the left axes; the mean difference is plotted on a floating axis on the right as a bootstrap sampling distribution. The mean difference is depicted as a dot; the 95% confidence interval is indicated by the ends of the vertical error bar. C, D, Estimation statistic corresponding to Figure 3E,F. The mean difference between Tlr4LoxTB-Saline and Tlr4LoxTB-LPS for food (A) and weight change (B) are shown in the Gardner–Altman estimation plot. Both groups are plotted on the left axes; the mean difference is plotted on a floating axis on the right as a bootstrap sampling distribution. The mean difference is depicted as a dot; the 95% confidence interval is indicated by the ends of the vertical error bar. E, F, Estimation statistics corresponding to Figure 3H,I. The mean difference between restricted-saline and restricted-LPS for food (E) and weight change (F) are shown in the Gardner–Altman estimation plot. Both groups are plotted on the left axes; the mean difference is plotted on floating axes on the right as a bootstrap sampling distribution. The mean difference is depicted as a dot; the 95% confidence interval is indicated by the ends of the vertical error bar. Download Figure 3-1, TIF file.

TLR4 is selectively enriched in CGRP-positive afferents

Although Tlr4 mRNA has previously been described in the nodose ganglion (Hosoi et al., 2005), little information is available regarding its exact cellular distribution. Chromogenic ISH allowed us to detect the signal for the Tlr4 transcript as a red precipitate (FastRed) visible under brightfield and fluorescent illuminations. The majority of the cellular profiles in the nodose and jugular ganglia was Tlr4-negative or contained very few Tlr4 signals (Fig. 4A–D). Diffuse and weak Tlr4 signals were seen in both neurons and non-neuronal cells in the nodose-jugular ganglia and the vagal trunk itself. However, a subset of cells resembling neurons contained moderate to high levels of Tlr4 signals (Fig. 4A-D). Notably, neurons with high expression levels were not uniformly distributed but were concentrated in the jugular ganglion and the rostral pole of the nodose ganglion (Fig. 4A–D). The caudal nodose ganglion itself, which contains a large population of vagal neurons including gastrointestinal afferents, was largely devoid of Tlr4-positive neurons. Based on our estimates, <12% of vagal afferents located in the rostral nodose ganglion proper contained moderate to high levels of Tlr4 signals (Fig. 4D). Considering that the boundary between the nodose and jugular-petrosal ganglia is ambiguous, many Tlr4-positive neurons observed in the nodose ganglion may have been part of the jugular ganglion. Indeed, over 50% (n = 3) of vagal afferents in the jugular ganglion were Tlr4-positive and close to 25% (n = 3) of them contained moderate to high levels of Tlr4.

Figure 4.
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Figure 4.

A–D, Chromogenic ISH for Tlr4 mRNA (red) in the nodose and jugular ganglia of C57Bl6/J mice. Using fluorescent illumination, scattered positive cells resembling neurons are observed mostly in the jugular portion of the ganglionic complex (white arrows). Under bright-field illumination, representative neuronal profiles were labeled as expressing Tlr4 signals at high (H), medium (M), or low levels (L). Cells with no signals were also apparent (N). Black arrows indicate putative non-neuronal cells surrounding neuronal profiles. Tissues were counterstained with hematoxylin (purple) to facilitate the identification of cellular profiles. The scale bars in B applies to C. D, Graph showing the percentage of neuronal profiles with different ISH signal strengths for Tlr4. Data are expressed as the mean percentage ± maximal values of Tlr4-expressing neurons in the nodose and jugular ganglia. The total number of counted profiles is indicated above each bar graph. The Extended Data Figure 4-1 includes a control of specificity for the Tlr4 probe. c, caudal; H, high intensity signal; HS, hematoxylin stain; M, medium intensity signal; N, no visible signal; cNG, caudal nodose ganglion; rNG, rostral nodose ganglion; JG, jugular ganglion; L, low intensity signal; r, rostral.

Extended Data Figure 4-1

The specificity of the probe used to detect Tlr4 was validated by comparing by chromogenic ISH for Tlr4 mRNA (red) the ganglia obtained from mice with a globally reactivated allele Tlr4LoxTB (A, B) to that of Tlr4LoxTB mice (C, D). It is evident that robust red precipitates accumulated in Tlr4-positive neuronal profiles indicated by black arrows in reactivated mice. In contrast, the ganglion of Tlr4LoxTB mice was almost entirely devoid of Tlr4 signals. Tissues were counterstained with hematoxylin (purple) and images were collected either under brightfield illumination. The scale bar in A applies to C. JG, jugular ganglion. Download Figure 4-1, TIF file.

Our next studies sought to confirm the neuronal identity of Tlr4-expressing cells. Specifically, Nav1.8-CreChR2-YFP was used to identify the cell bodies of vagal Nav1.8 neurons. Chromogenic ISH was combined with GFP immunohistochemistry to visualize neurons co-expressing Nav1.8 and Tlr4 (Fig. 5A–F). When combined with the detection of ChR2-labeled cells, it became evident that Tlr4 mRNA was expressed by Nav1.8 neurons (Fig. 5D,E). In the nodose-jugular ganglia, ∼84% (n = 4) of Tlr4-expressing cells were also Nav1.8-positive. Conversely, ∼9% (n = 4) of Nav1.8-positive neurons expressed Tlr4. The jugular ganglion and rostral nodose ganglion are known to contain vagal afferents producing CGRP (Helke and Niederer, 1990; Mazzone and Undem, 2016). Therefore, we next performed double chromogenic ISH to simultaneously detect Tlr4 and either Calca (CGRP precursor gene) or CGRP peptide. As anticipated, both Tlr4 and Calca-expressing neurons were the most abundant in the jugular ganglion and the most rostral portion of the nodose ganglion (Fig. 6A–D). Tlr4 mRNA signals often accumulated in Calca-expressing neurons (Fig. 6B,C), with an estimated 40% (n = 4) of Tlr4-expressing neurons also being Calca-positive (Fig. 6E). In summary, our anatomic data established that Tlr4 was predominantly, but not exclusively, expressed by a subset of vagal afferents producing Calca, which are known to be mainly airways and facial somatosensory and nociceptive afferents (Helke and Niederer, 1990; Mazzone and Undem, 2016).

Figure 5.
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Figure 5.

A, B, Chromogenic ISH for Tlr4 mRNA (red) in the rostral nodose ganglion of the Nav1.8-CreChR2-YFP mouse. Immunolabeling for GFP (green) was used to label the cell bodies of Nav1.8 neurons. C, D, At higher magnification (digital z-stack acquired by confocal microscopy), three large cells profiles with high intensity Tlr4 signals can be seen. The white arrowhead indicates scattered Tlr4 signal interpreted as a putative non-neuronal cell. E, Plot profiles of the distribution across two cells represented in D. The red and green lines correspond to the fluorescence intensity for Tlr4 and YFP, respectively. Notably, Tlr4 signals strongly accumulated within the boundary of ChR2-YFP-labeled neurons. F, Absolute numbers of counted cell profiles. From these data, it was concluded that Tlr4 was primarily co-expressed by Nav1.8 neurons. cNG, caudal nodose ganglion; rNG, rostral nodose ganglion; JG, jugular ganglion; X, vagus nerve trunk.

Figure 6.
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Figure 6.

A, Camera lucida drawing of one vagal ganglionic complex processed for double chromogenic ISH for Tlr4 and Calca mRNAs. Each symbol represents one individual neuronal profile identified as positive for Tlr4 (red dots), Calca (open circles), or both signals (circles filled with red). Note that both Tlr4-positive and Calca-positive cells preferentially accumulated in the rostral pole of the ganglionic mass. B–D, Representative bright-field images of doubly labeled neurons for Tlr4 (red) and Calca (blue) in the ganglionic mass of C57Bl6/J mice. Overall, these data indicate that Tlr4 and Calca were frequently co-expressed in the same neurons. The numbers are meant to indicate four examples of profiles co-expressing Calca and Tlr4 mRNAs. Ganglia were counterstained with hematoxylin. E, Quantification of Tlr4 signals (dots) in cell profiles negative (left) or positive (right) for Calca. Individual cell profiles are displayed as black circles. A total of 923 cell counterstained profiles were counted, n = 4 mice. Using ImageJ, the circumference of each profile was determined and further used to categorize cell profiles into small or large/medium cells. Absolute numbers of tlr4-positive profiles is also indicated above each graph. Scale bar in C applies to D. cNG, caudal nodose ganglion; rNG, rostral nodose ganglion; JG, jugular ganglion; X, vagus nerve trunk.

CGRP is an immunomodulatory peptide released from peripheral afferents during bacterial infections (Lai et al., 2017). Thus, we tested whether TLR4 signaling is sufficient and/or required for the release of CGRP from cultured vagal afferents. As anticipated, the application of LPS robustly raised CGRP release (Fig. 7A,B). In Tlr4LoxTB mice, no significant changes in CGRP release were noticed following LPS. In the ganglia of Nav1.8-restricted Tlr4 mice, the application of LPS raised CGRP release to the same degree as in the ganglia of control mice (Fig. 7A,B). These data demonstrated that TLR4 signaling in vagal afferents directly mediates CGRP release on LPS exposure. Of note, the application of LPS did not alter the transcription of Tlr4 and Calca in sensory ganglia cultured for 5 d (see Extended Data Figure 7-1).

Figure 7.
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Figure 7.

A, LPS-induced CGRP production from Tlr4-expressing vagal afferents. CGRP ELISA from nodose-jugular ganglia organotypic cultures isolated from WT (controls), Tlr4LoxTB (KO), and Tlr4LoxTB X Nav1.8-Cre (restricted) animals and treated with LPS (500 ng/ml) or vehicle (sterile saline). Groups consists of ganglia from n = 5–7 animals. Data were analyzed using one-way ANOVA followed by Tukey’s post hoc test. Different letters indicate significant differences between columns. Data are all expressed as the mean values ± SEM. B, Estimation statistics we included as the mean difference for three comparisons are shown in the Cumming estimation plot. The raw data are plotted on the upper axes; each mean difference is plotted on the lower axes as a bootstrap sampling distribution. Mean differences are depicted as dots; 95% confidence intervals are indicated by the ends of the vertical error bars. In Extended Data Figure 7-1, we further assessed the expression levels for Tlr4 and Calca in dorsal root ganglia cultured for 5 d.

Extended Data Figure 7-1

Q-PCR analysis using Taqman primers (see main text) of dorsal root ganglion (DRG) either freshly collected from WT mice or cultured for 5 d. In all samples, Tlr4 and Calca mRNAs were detected at moderate levels with averaged Ct values of 32. Notably, after 5 d in culture, levels of Tlr4 were robustly stimulated, but Calca remained unchanged. When LPS was applied as described in the main text, Tlr4 and Calca expression levels remained unchanged. Download Figure 7-1, TIF file.

Discussion

Novel tools for the study of TLR4

LPS is a potent inducer of proinflammatory molecules, a number of which share the same cellular targets and intracellular signaling pathways as TLR4 (Miyamoto and Verma, 1995; Muta et al., 2002; Skelly et al., 2013). Combined with the redundancy of cells expressing TLR4 throughout the body (Poltorak et al., 1998a; Lin et al., 2000; Laflamme and Rivest, 2001; Liu et al., 2002), this has made it particularly difficult to tease apart the cellular and molecular determinants of TLR4 actions. Here, we developed and validated a novel mouse model to manipulate TLR4 expression in a cell type-specific manner within the context of intact animals. In the current study, we used this model to investigate the role played by peripheral afferents in LPS-induced anorexia. In theory, this approach can be used to test TLR4 signaling in any genetically-targeted cells provided that a Cre line is available. It has also been difficult to study TLR4 actions because of the lack of validated anti-TLR4 antibodies. While radioisotopic ISH has been used to detect Tlr4-expressing cells in the CNS (Laflamme and Rivest, 2001; Chakravarty and Herkenham, 2005), the present study has refined these techniques to specifically analyze Tlr4 gene expression in vagal afferents. In particular, we used a highly sensitive ISH procedure called RNAscope (Advanced Cell Diagnostic; Wang et al., 2014; Grabinski et al., 2015). The latter technique allowed us to detect Tlr4 mRNA with virtually no background. It also allowed us to easily detect Tlr4 in combination with other transcripts or GFP.

Role of vagal afferents during inflammatory anorexia

It is likely that peripheral afferents can rapidly convey information to the brain relevant to a potentially lethal threat, such as a Gram-negative bacterial infection. This phenomenon might possibly exist throughout the animal kingdom because sensory neurons in Aplysia sea slugs have been reported to be sensitive to bacterial LPS (Clatworthy and Grose, 1999). In rodents, numerous studies have suggested the ability of both vagal and spinal sensory neurons to respond to a broad range of inflammatory stimuli (Goehler et al., 1997; Ek et al., 1998; Ross et al., 2003; Eijkelkamp et al., 2010; La and Gebhart, 2011; Chiu et al., 2013). Prior studies have established that rodents with bilateral subdiaphragmatic surgical vagotomy showed diminished anorexia in response to LPS (Bret-Dibat et al., 1995; Sergeev and Akmaev, 2000). However, despite all of the evidence implicating peripheral afferents in sensing LPS, the requirement of vagal afferents in mediating LPS-induced anorexia has been difficult to demonstrate. For instance, other studies have reported identical degrees of LPS-induced anorexia in animals with capsaicin-mediated or surgery-mediated deafferentation (Schwartz et al., 1997; Porter et al., 1998b; Wieczorek et al., 2005). To our knowledge, prior in vivo studies have rarely examined LPS-induced anorexia before the 1-h postinjection time point (Kent et al., 1992; Liu et al., 2016). However, our data reinforced the view that anorexia is initiated rapidly after intraperitoneal LPS exposure. This observation led us to hypothesize that first, LPS may directly act on vagal afferents to suppress feeding; and second, that prior studies may have missed the early anorectic response to LPS. Contrary to expectation, our findings indicate that TLR4 signaling in peripheral afferents alone is not sufficient to initiate anorexia. As explained below, we believe these findings can be explained by the fact that TLR4 signaling occurs mostly in rostral nodose and jugular vagal afferents supplying the airways, rather than those involved in feeding. Instead, it is likely that LPS-induced anorexia is initiated by the de novo production of cytokines and prostaglandins within the brain (Yao et al., 1999; Layé et al., 2000; Sachot et al., 2004; Chakravarty and Herkenham, 2005; Ching et al., 2007; Elander et al., 2007; Rummel et al., 2008; Skelly et al., 2013). A recent single-cell sequencing study determined that the molecular make-up of jugular afferents is reminiscent of that of spinal nociceptors rather than vagal afferents of the nodose ganglion (Kupari et al., 2019). Thus, the difference of expression of Tlr4 mRNA in the nodose versus jugular ganglion is not completely surprising. Considering that the jugular and nodose ganglia are fused in rodents, one prior report of Tlr4 expression in the whole nodose ganglion may have been because of a jugular contamination (Hosoi et al., 2005).

TLR4 signaling and vagal CGRP

In the CNS, TLR4 is enriched in non-neuronal cells, most notably including microglial cells (Lacroix et al., 1998; Laflamme and Rivest, 2001; Lehnardt et al., 2002; Olson and Miller, 2004; Chakravarty and Herkenham, 2005; Kigerl et al., 2007; Madore et al., 2013; Larochelle et al., 2015). Several laboratories also reported TLR4 expression in neurons (Rolls et al., 2007; Yoo et al., 2011; Leow-Dyke et al., 2012; Calvo-Rodríguez et al., 2017). However, the latter studies relied on in vitro data and/or unvalidated antibodies against TLR4. In contrast, high resolution ISH mapping studies have established that TLR4 expression was restricted to non-neuronal cells in normal, inflamed, and injured CNS (Laflamme and Rivest, 2001; Chakravarty and Herkenham, 2005; Kigerl et al., 2007). Hence, a general agreement is that CNS neurons do not express TLR4, or they do at very low levels. In contrast, TLR4 expression was found to be relatively high in a small subset of vagal afferents. Notably, we found that LPS did not significantly increase Nfκbia expression in Tlr4-expressing Nav1.8 neurons. This result is in agreement with the fact that LPS is a poor inducer of the NF-κB pathway in neurons compared with other cell types (Quan et al., 1997; Stern et al., 2000; Laflamme and Rivest, 2001; Chakravarty and Herkenham, 2005; Kigerl et al., 2007; Listwak et al., 2013). In addition, NFκB-independent signaling mechanisms may account for LPS actions on sensory neurons (Li et al., 2014; Meseguer et al., 2014; Allette et al., 2017; Boonen et al., 2018). This putative non-genomic effect of TLR4 on peripheral afferents differs from its canonical NFκB-dependent actions on immune, endocrine, and glial cells. Thus, additional studies are warranted in our Nav1.8-restricted TLR4 mice to elucidate LPS sensing mechanisms in vagal afferents. Considering the large amount of LPS that is constitutively contained within the gut lumen (Hersoug et al., 2016), we anticipated that TLR4 would be expressed by vagal afferents supplying the intestines. Based on our data, we cannot rule out entirely that certain vagal afferents innervating the gut expressed TLR4. In fact, a small subset of CGRP-positive vagal afferents was reported to supply the rodent stomach (Bai et al., 2019). Nonetheless, it must be stressed that Tlr4 mRNA was rarely seen in neurons of the caudal nodose ganglion, where reside vagal afferents innervating the gut. Thus, the common proposition that LPS signaling in vagal afferents sense gut microbial communities is not supported by our findings. Instead, we found abundant expression of TLR4 in CGRP-producing vagal afferents, a subgroup of vagal afferents supplying the upper airways, meninges, and facial skin (Mazzone and Undem, 2016). Moreover, our in vitro data indicate that TLR4 signaling is required and sufficient to mediate LPS-induced CGRP release. Interestingly, bacterial infections in the respiratory tract are common and can lead to sepsis more often than in any other primary sites of infection. CGRP is a well-known immuno-modulatory peptide released from vagal and spinal C-fiber endings (Myers et al., 1996; Abbadie et al., 2002; Abrahamsen et al., 2008; Chiu et al., 2013). Vagal afferents play a key role in airways defenses and coughing (Prescott et al., 2020; Ruhl et al., 2020). Therefore, it is tempting to speculate that the stimulation of TLR4 in vagal airway afferents plays an important immuno-modulatory role in the airways and lungs. For instance, a recent study demonstrated that the release of CGRP from respiratory vagal afferents is critical to bacterial clearance in lungs (Baral et al., 2018). Therefore, further studies in TLR4 reactivated mice are warranted to verify their susceptibility to respiratory infections. Our prediction is that the stimulation of TLR4-bearing vagal (and/or spinal) neurons supplying the airways during a Gram-negative bacterial infection would trigger an efferent response, probably involving the release of CGRP, aimed at containing inflammation and favoring bacterial clearance. It is without saying that molecules other than TLR4 may also be involved in mediating CGRP release during a bacterial infection, as suggested by several studies (Diogenes et al., 2011; Meseguer et al., 2014; Boonen et al., 2018). In particular, Meseguer and colleagues showed that the release of CGRP from isolated tracheas is a TRPA1-dependent phenomenon. However, based on data obtained with a Trpa1 global knock-out, it cannot be certain that neuronal TRPA1 was involved in the observed effects. Indeed, CGRP is also released from immune cells (Baliu-Piqué et al., 2014) and TRPA1 itself has been reported in immune and epithelial cells (Khalil et al., 2018). Moreover, the high dose of LPS used in this prior TRPA1-related study is believed to cause TLR4-independent effects because of LPS interacting with the membrane itself (Redeker and Briscoe, 2019; Yin et al., 2020). Using a lower dose of LPS, other authors have shown TRPA1 to be dispensable for LPS calcium imaging response in sensory neurons (Diogenes et al., 2011; Boonen et al., 2018). Once again, the advantage of our model is that we manipulated TLR4 only in Nav1.8 neurons of the nodose ganglion. In other words, it is likely that TLR4 and TRP channels are synergistically involved in the release of CGRP from neuronal endings, but TRP channels do not mediate this response alone. Lastly, it must be noted that the transcriptional profile of jugular vagal afferents resembles that of spinal and trigeminal nociceptors (Kupari et al., 2019). It is therefore likely that our findings may be extrapolated to other Tlr4-expressing peripheral nerves including spinal and trigeminal CGRP-positive afferents.

Acknowledgments

Acknowledgements: We thank Abhijit Bugde from The University of Texas Southwestern Live Cell Imaging Core Facility for his help with confocal microscopy, Dr. John Wood (University College London) for kindly providing us with Nav1.8-Cre mice, and Claudia Vianna, Arely Tijano, Rebekah Brown, and Newaz Ahmed for their technical assistance (The University of Texas Southwestern).

Footnotes

  • The authors declare no competing financial interests.

  • L.J. was supported by the National Institutes of Health Grant K01AA024809. L.G. was supported by the Irwin & Irma Grossman Research Fund for Type I Diabetes (University of Texas Southwestern). M.D.B. was supported by the National Institutes of Health Grant K22NS096030, The American Pain Society Future Leaders grant, and the Rita Allen Foundation. The microcopy facility is supported by the National Institutes of Health Award 1 S10 OD021685-01A1 to Kate Luby-Phelps.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Synthesis

Reviewing Editor: Quentin Pittman, University of Calgary

Decisions are customarily a result of the Reviewing Editor and the peer reviewers coming together and discussing their recommendations until a consensus is reached. When revisions are invited, a fact-based synthesis statement explaining their decision and outlining what is needed to prepare a revision will be listed below. The following reviewer(s) agreed to reveal their identity: Karel Talavera, Cristian Acosta.

The view shared by both reviewers is that both the time course of TLR4 (particularly with regards to the actual levels of the receptor between the 2 systems) and a real quantitation using proper phenotypic markers for neuronal and non-neuronal cells are essential to lend credence to what you conclude. The antibody characterization can be saved if you can provide strong and convincing published data on its specificity. Please note also the comments with respect to the statistics as follows:

First, there is a complete lack of power calculations, so the size of the experimental groups seems a bit random (and it is reported vaguely, i.e. 3 to 7 mice, not indicating clearly how many exactly in each condition). Second, the data were not checked for normal distribution of the probability function (usind D’Agostino-Pearson, for example) and thus, we can not ascertain whether or not non-parametric tests would have been better suited to analyze the data. However, and given the small size of the groups, I would strongly expect that to be the case.

The actual reviews are appended below, each of which raises additional issues that must be addressed in the revision

Reviewer 1:

The main contribution of this study is the generation of mouse models that allow inquiring over the roles of TLR4 receptors in sensory neurons. It also sheds some light on the mechanisms underlying the effects of LPS, i.e., anorexia and CGRP release from sensory neurons.

1. Line 31, it is stated that “Toll-like receptor 4 (TLR4) is the receptor for lipopolysaccharides (LPS)”. This implies a picture that is too simplified, as actually MD-2 is the main LPS binding module of the TLR4-MD-2 receptor complex. In addition, as noted by the authors, several TRP channels are activated by LPS in a TLR4-independent manner (e.g., TRPA1, Meseguer et al. Nat Comm 2014 and TRPV4, Alpizar et al. Nat Comm 2017).

2. Lines 36-39, it is not clear why the fact that LPS triggers anorexic within minutes and that it does not cross the blood-brain barrier directly suggests that the implication of a peripheral vagal-mediated mechanism. The evidence for this comes in the next sentences.

3. Line 49, please avoid teleologic style, the preservation of a mechanism during evolution does not have any aim. See also line 348, it is not necessary to use a teleological point of view to describe biological phenomena.

4. Lines 56-58, none of the studies of Meseguer et al. and Boonen et al. contradict the functional expression of TLR4 in vagal afferents. These studies looked at intracellular Ca2+ in cultured DRG and nodose neurons and showed that some TRP channels can be activated by LPS, leading to acute pain and neurogenic inflammation in a TLR4-independent manner.

5. I would like to understand better this point, in lines 245 and 245 the authors state that “Crossing Tlr4loxTB mice with Zp3-Cre mice produced mice in which expression of endogenous Tlr4 was globally reactivated in all cells.” Is this in ALL cells of the body, even in those where no TLR4 is normally expressed?

6. In the same lines, please, specify how LPS was administered to induce the LPS-induced inflammatory response. Please, make sure that the details of LPS application are given for every experiment.

7. Lines 246 and 247, please change the use of “identical” to “not-significantly different”.

8. Line 250, specify how LPS and how much was administered.

9. Line 254, please write “To test for functional TLR4 signaling in vagal afferents in vivo”.

10. Line 257, delete “we showed that” or write it in present tense.

11. Lines 260-262, on what data do the authors support the statement “Higher Tlr4 levels in controls compared to Nav1.8-restricted Tlr4 mice were likely due to Tlr4 expression in non-neuronal cells.”

12. Line 262, write “show” instead of “showed", otherwise the reader may think that this was done in a previous study.

13. Lines 267-268, delete “More surprisingly,” and “However,” in line 269. A result is not surprising if the authors can already advance an explanation from previous studies.

14. Lines 271-272, it is not clear what data confirms the neuronal identity of Tlr4-expressing cells in the nodose ganglion. In fact, the sentence in lines 260-262 “Higher Tlr4 levels in controls compared to Nav1.8-restricted Tlr4 mice were likely due to Tlr4 expression in non-neuronal cells.” seems to contradict this statement.

15. Lines 272-273, it is claimed that the authors “established the successful re-expression of Tlr4 in vagal afferents” but only ganglia were tested for expressing, not nerve fibers.

16. Line 308, it is stated that “approximately 84% (n=4) of Tlr4-expressing cells were also Nav1.8-positive”. Isn’t the other 16% negative for Nav1.8, contradicting another statement given below “we never observed Tlr4-expressing neurons that were Nav1.8-negative”?

17. This co-expression descriptions should be backed-up with actual numbers of neurons expressing the different markers.

18. Lines 315-317, the finding that “an estimated 38% (n=3) of Calca-expressing neurons also being Tlr4-positive” does not tell about how much Tlr4 mRNA signals preferentially accumulate in Calca-expressing neurons, but vice versa.

19. Lines 319-320, the data provided does not show that as claimed “Tlr4 was mainly expressed by a small subset of vagal afferent C-fibers producing CGRP”. Please, note also that the co-expression study was performed at the level of the ganglia, not at the level of afferent C-fibers.

20. It is recommended that Venn diagrams are used for better representation of all co-expression data.

21. Line 322, delete “know”.

22. Line 324, please note that the CGRP release determinations were not performed in “cultured vagal afferents” but in cultured ganglia. For the same reason the conclusion given in lines 328-329 about vagal afferents is not supported.

23. It is unclear why the no reference is made to the results of Meseguer et al. (Nat Comm., 2014) showing that the CGRP release triggered by LPS from isolated mouse trachea was entirely dependent on TRPA1.

Reviewer 2:

The main advance is the generation and validation of a new transgenic mouse model that could be helpful to study the role of TLR4 in a subpopulation of neurons expressing Nav1.8 (albeit the authors did not do a characterization of the expression of the receptor in either DRG or trigeminal neurons, the other sites of high Nav1.8 expression). In terms of the role of TLR4 as mediator in the release of CGRP, the results are not that novel (others have shown that LPS acting via TLR4/MD2 can trigger the release of neuropeptides from DRG, nnodose and trigeminal neurons). As the authors postulates a role for this loop of LPS-TLR4-CGRP in nodose and jugular neurons in fighting airway infections but provide no proof of that, the advance to the field is present but it is minimal (barely incremental).

The starting point of the study was to examine the involvement of TLR4 expressed by Nav1.8+ neurons in LPS stimulated rapid-onset anorexia. This rendered a surprisingly negative result. The authors decided to study the expression pattern of TLR4 in vagal afferents other than those involved in innervating the gut and ended up discovering a subtype of TLR4+ Nav1.8+ CGRP+ neurons that release CGRP when exposed to LPS.

They also present data that validate a new transgenic mouse model of reactivable TLR4, that can make a valuable contribution to further studies that focus on Nav1.8-expressing neurons and the role that TLR4 may have on them.

This is a simple, straight forward study. My concerns follows:

1. As a general comment, please refrain from stating that all Nav1.8-expressing neurons are unmyelinated as this is clearly not the case (Djouhri et al., 2003). In fact, Nav1.8 is expressed significantly in nociceptive myelinated A-fibers.

2. In the Significance Statement the authors state that their findings could explain “How nerves modulate bacterial infections”. But I wonder, is it not the other way around? The bacterial infection presumably triggers the release of CGRP via activation of TLR4, thus in any event it is a bidirectional phenomenon.

3. A diagram explaining the experimental design would aid the presentation of the different experimental procedures carried out in this study.

4. qPCR must be reported in full - and the authors must abide by the standards of publication using this technique, particularly as they used the ΔΔ2CT method (Bustin et al., 2010). This means providing data on efficiency curves, melting curves and the sequences for all primers used, plus how quality of the mRNA was determined, how much of total RNA was used, etc.

5. Positive and negative controls for the immunostaining are missing and must be included to appreciate the real magnitude of the background staining. Full characterization of the anti-CGRP antibody must also be included.

6. What intensity was considered to label a cell as “positively stained”? How was that intensity determined? How many sections were analyzed and from how many animals? How many images per ganglia? This must be disclosed in full.

7. Why were ganglia cultured for 5 to 7 days? The authors must provide a convincing argument to do this as time in culture profoundly alters the phenotype of the neurons, among other parameters. Proof that TLR4 is not down regulated or upregulated by the culture conditions must be provided. Same goes for CGRP levels. What about measuring the ability of LPS to trigger the release of CGRP in acutely dissociated neurons? Or in their same organotipic preparation but at shorter times?

8. The study lacks power calculations.Also, normal distribution of probability for each data set should have been performed. Non-parametric tests are often better suited to compare data with non-normal distributions.

9. Markers of neurons and non-neuronal cells would have been far more convincing. Also some other positive tissue with high levels of TLR4 should have been used for comparison. In 5C there are some accumulations of signal suggesting at least some mRNA for TLR4 is still present despite the genetic suppression. Is it in non-neuronal cells? This should be demonstrated.

10. The quantitative approach of the authors to report the proportions and numbers of neurons or cells expressing TLR4 (or calca) mRNA is not convincing enough - albeit the authors state that they are reporting only estimations. However, I believe they can do much better and do a proper measurement, with objective rather than subjective measurements.It would be interesting to see lower magnification images of the contents shown in Figure 6.

Minor comments

Lines 307 to 310: The authors state that 84% of TLR4-expressing neurons were Nav1.8+. Therefore there were some TLR4-expressing neurons that were negative for Nav1.8. This needs to be corrected.

Please add the dose of LPS in plots 1C and 1D. Sample sizes are variable in 1C with some of the columns representing data from just 3 mice. Non parametric statistics should have been used in that case. I would also prefer to see scatter plots to have an idea of how homogeneous the levels of TNF-a are.

Correct the statement in line 322.

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TLR4 Signaling Selectively and Directly Promotes CGRP Release from Vagal Afferents in the Mouse
Lin Jia, Syann Lee, Jessica A. Tierney, Joel K. Elmquist, Michael D. Burton, Laurent Gautron
eNeuro 14 December 2020, 8 (1) ENEURO.0254-20.2020; DOI: 10.1523/ENEURO.0254-20.2020

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TLR4 Signaling Selectively and Directly Promotes CGRP Release from Vagal Afferents in the Mouse
Lin Jia, Syann Lee, Jessica A. Tierney, Joel K. Elmquist, Michael D. Burton, Laurent Gautron
eNeuro 14 December 2020, 8 (1) ENEURO.0254-20.2020; DOI: 10.1523/ENEURO.0254-20.2020
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