The Effect of Inclusion Criteria on the Functional Properties Reported in Mouse Visual Cortex

Example cells that pass inclusion criteria exclusively. A, All DF/F responses to the preferred stimulus condition (TF and direction) of a cell that passes all published inclusion criteria. B, Heatmap of mean %DF/F responses to each stimulus condition (TF × direction). C, Mean %DF/F responses (± SEM) to stimuli of different grating directions in the same example cell. D–F, Same as in A but with a cell that passes most criteria, but not Study 2. G–I, Same as in A but with a cell that only passes Study 1 criteria.

Most studies select for neurons along similar axes of the data. A, Six density plots of the mean response at the preferred stimulus condition (%DF/F) against the SD of the responses at the preferred stimulus condition where each point represents a single neuron. For each study, colored neurons are those selected for by inclusion criteria. Heatmap represents the density of neurons. B–D, Tuning characteristics after inclusion criteria are applied to Allen Brain Observatory. B, Mean TF tuning of six visual areas when different inclusion criteria are applied. C, D, show the mean OSI and DSI of six visual areas, respectively. E, Venn Diagram of neurons that were selected for by each inclusion criteria. Area of circles represents the number of neurons. Letters indicate example neurons from Figure 2.

Tuning characteristics of neurons based on robustness. A, E, I, Mean TF, OSI, and DSI tuning of neurons in V1, AL, and PM based on what percentage of most robust cells (cells with low CV) are included in the analysis. Shaded regions indicate SEM. The minimum percentage most robust cells displayed is 5%. B, Distribution of TF tuning of 10% least robust cells. C, Distribution of TF tuning of 10% most robust cells. F, G, J, K, Same as in B, C but with OSI and DSI. D, H, L, Mean TF, OSI, and DSI tuning of neurons in all visual areas comparing the 10% most robust neurons to the entire population of neurons. M, Heat map displaying p values for Mann–Whitney U test comparing the 10% most robust neurons and the entire population of neurons. The color scale is centered at p = 0.05/6 to account for Bonferroni correction. N, Mean DSI calculated for neurons selected to match the mean CV for each the neurons selected by each criterion, for each area, compared with the mean DSI for the neurons selected by that criteria and area. O, P, Same as in M but for OSI and TF.

Tuning characteristics of neurons based on robustness with cross-validated metrics. A, E, I, Mean TF, OSI, and DSI tuning of neurons in V1, AL, and PM based on what percentage of neurons are included in the analysis, starting with the most robust neurons. Shaded regions indicate SEM. The minimum percentage most robust cells displayed is 5%. B, Distribution of TF tuning of 10% least robust neurons. C, Distribution of TF tuning of 10% most robust neurons. F, G, J, K, Same as in B, C but with OSI and DSI. D, H, L, Mean TF, OSI, and DSI tuning of neurons in all visual areas in 10% most robust neurons versus the entire population of neurons.