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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

P2X-GCaMPs as Versatile Tools for Imaging Extracellular ATP Signaling

Matthias Ollivier, Juline Beudez, Nathalie Linck, Thomas Grutter, Vincent Compan and Francois Rassendren
eNeuro 30 December 2020, 8 (1) ENEURO.0185-20.2020; https://doi.org/10.1523/ENEURO.0185-20.2020
Matthias Ollivier
1Institut de Génomique Fonctionnelle, Université de Montpellier, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Montpellier, France
3LabEx Ion Channel Science and Therapeutics, Montpellier, France
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Juline Beudez
2Centre National de la Recherche Scientifique, Laboratoire de Conception et Application de Molécules Bioactives Unité Mixte de Recherche 7199, Équipe de Chimie et Neurobiologie Moléculaire, Université de Strasbourg, Strasbourg, France
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Nathalie Linck
1Institut de Génomique Fonctionnelle, Université de Montpellier, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Montpellier, France
3LabEx Ion Channel Science and Therapeutics, Montpellier, France
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Thomas Grutter
2Centre National de la Recherche Scientifique, Laboratoire de Conception et Application de Molécules Bioactives Unité Mixte de Recherche 7199, Équipe de Chimie et Neurobiologie Moléculaire, Université de Strasbourg, Strasbourg, France
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Vincent Compan
1Institut de Génomique Fonctionnelle, Université de Montpellier, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Montpellier, France
3LabEx Ion Channel Science and Therapeutics, Montpellier, France
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Francois Rassendren
1Institut de Génomique Fonctionnelle, Université de Montpellier, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Montpellier, France
3LabEx Ion Channel Science and Therapeutics, Montpellier, France
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0185-20.2020
PubMed 
33380526
Published By 
Society for Neuroscience
History 
  • Received May 9, 2020
  • Revision received November 2, 2020
  • Accepted December 7, 2020
  • Published online December 30, 2020.
Copyright & Usage 
Copyright © 2021 Ollivier et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Matthias Ollivier1,3,
  2. Juline Beudez2,
  3. Nathalie Linck1,3,
  4. Thomas Grutter2,
  5. Vincent Compan1,3,* and
  6. Francois Rassendren1,3,*
  1. 1Institut de Génomique Fonctionnelle, Université de Montpellier, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Montpellier, France
  2. 2Centre National de la Recherche Scientifique, Laboratoire de Conception et Application de Molécules Bioactives Unité Mixte de Recherche 7199, Équipe de Chimie et Neurobiologie Moléculaire, Université de Strasbourg, Strasbourg, France
  3. 3LabEx Ion Channel Science and Therapeutics, Montpellier, France
  1. Correspondence should be addressed to Francois Rassendren at francois.rassendren{at}igf.cnrs.fr or Vincent Compan at Vincent.compan{at}igf.cnrs.fr.
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Author contributions

  1. Author contributions: M.O., J.B., V.C., and F.R. designed research; M.O., J.B., N.L., T.G., V.C., and F.R. performed research; M.O., J.B., N.L., T.G., V.C., and F.R. analyzed data; M.O., T.G., V.C., and F.R. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the Agence National de la Recherche Grant ANR-14-CEE11-0004-01 and the LabEx ICST Grant ANR-11-LABX-0015.

  • ↵* V.C. and F.R. are equally participating senior authors.

Funding

  • Agence Nationale de la Recherche (ANR)

    ANR-14-CEE11-0004-01; ANR- 11-LABX-0015

Other Version

  • You are viewing the most recent version of this article.
  • previous version (December 30, 2020).

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Jul 20251511129
Aug 2025613624
Sep 2025920418
Oct 20251414830
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Total 2025691334231
Total97761411560
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P2X-GCaMPs as Versatile Tools for Imaging Extracellular ATP Signaling
Matthias Ollivier, Juline Beudez, Nathalie Linck, Thomas Grutter, Vincent Compan, Francois Rassendren
eNeuro 30 December 2020, 8 (1) ENEURO.0185-20.2020; DOI: 10.1523/ENEURO.0185-20.2020

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P2X-GCaMPs as Versatile Tools for Imaging Extracellular ATP Signaling
Matthias Ollivier, Juline Beudez, Nathalie Linck, Thomas Grutter, Vincent Compan, Francois Rassendren
eNeuro 30 December 2020, 8 (1) ENEURO.0185-20.2020; DOI: 10.1523/ENEURO.0185-20.2020
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Keywords

  • ATP
  • biosensor
  • GECI
  • P2X
  • purinergic signaling

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