Extended Data Figure 6-2
ENaCα expression in Calhm3+Trpm5– sodium–taste cells in soft palate. Double-labeling in situ hybridization was performed to study expression of ENaCα in Calhm3+Trpm5– sodium–taste cells. A–C, ENaCα expression and that of Skn-1a (A), Calhm3 (B), and Trpm5 (C). Numbers of cells showing signals were counted, and the ratios of cells positive and negative for ENaCα (middle images) to the total population of cells positive for the gene (left images) are shown at the right (n = 3). White arrowheads indicate Skn-1a, Calhm3, or Trpm5 single-positive cells, and red arrowheads indicate cells co-expressing ENaCα with Skn-1a, Calhm3, or Trpm5. D, Robust decrease of ENaCα-expression in non-sour taste cells by Skn-1a deficiency in taste buds. Populations of Pkd2l1+ and Pkd2l1– cells in ENaCα-expressing cells were quantified by double-labeling in situ hybridization analyses. White and red arrowheads indicate representative Pkd2l1+ENaCα+ and Pkd2l1–ENaCα+ cells, respectively. Decrease of the Pkd2l1–ENaCα+ cell population was evaluated by Welch’s t test: p = 0.0009. Scale bars: 25 μm. Download Figure 6-2, TIF file.