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Research ArticleResearch Article: New Research, Neuronal Excitability

Geometry and the Organizational Principle of Spine Synapses along a Dendrite

Laxmi Kumar Parajuli, Hidetoshi Urakubo, Ai Takahashi-Nakazato, Roberto Ogelman, Hirohide Iwasaki, Masato Koike, Hyung-Bae Kwon, Shin Ishii, Won Chan Oh, Yugo Fukazawa and Shigeo Okabe
eNeuro 27 October 2020, 7 (6) ENEURO.0248-20.2020; DOI: https://doi.org/10.1523/ENEURO.0248-20.2020
Laxmi Kumar Parajuli
1Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan
2Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Tokyo 113-8421, Japan
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Hidetoshi Urakubo
3Department of Systems Science, Graduate School of Informatics, Kyoto University, Kyoto 606-8501, Japan
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Ai Takahashi-Nakazato
1Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan
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Roberto Ogelman
4Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045
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  • ORCID record for Roberto Ogelman
Hirohide Iwasaki
1Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan
5Department of Anatomy, Graduate School of Medicine, Gunma University, Gunma 371-8511, Japan
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Masato Koike
2Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Tokyo 113-8421, Japan
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Hyung-Bae Kwon
6Max Planck Florida Institute for Neuroscience, Jupiter, FL 33458
7Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205
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Shin Ishii
3Department of Systems Science, Graduate School of Informatics, Kyoto University, Kyoto 606-8501, Japan
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Won Chan Oh
4Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045
6Max Planck Florida Institute for Neuroscience, Jupiter, FL 33458
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Yugo Fukazawa
8Division of Brain Structure and Function, Research Center for Child Mental Development, Life Science Advancement Program, University of Fukui, Fukui 910-1193, Japan
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Shigeo Okabe
1Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0248-20.2020
PubMed 
33109633
Published By 
Society for Neuroscience
History 
  • Received June 8, 2020
  • Revision received October 2, 2020
  • Accepted October 7, 2020
  • Published online October 27, 2020.
Copyright & Usage 
Copyright © 2020 Parajuli et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Laxmi Kumar Parajuli1,2,
  2. Hidetoshi Urakubo3,
  3. Ai Takahashi-Nakazato1,
  4. Roberto Ogelman4,
  5. Hirohide Iwasaki1,5,
  6. Masato Koike2,
  7. Hyung-Bae Kwon6,7,
  8. Shin Ishii3,
  9. Won Chan Oh4,6,
  10. Yugo Fukazawa8 and
  11. Shigeo Okabe1
  1. 1Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan
  2. 2Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Tokyo 113-8421, Japan
  3. 3Department of Systems Science, Graduate School of Informatics, Kyoto University, Kyoto 606-8501, Japan
  4. 4Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045
  5. 5Department of Anatomy, Graduate School of Medicine, Gunma University, Gunma 371-8511, Japan
  6. 6Max Planck Florida Institute for Neuroscience, Jupiter, FL 33458
  7. 7Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, MD 21205
  8. 8Division of Brain Structure and Function, Research Center for Child Mental Development, Life Science Advancement Program, University of Fukui, Fukui 910-1193, Japan
  1. Correspondence should be addressed to Shigeo Okabe at okabe{at}m.u-tokyo.ac.jp.
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Author contributions

  1. Author contributions: L.K.P., H.I., M.K., H.-B.K., S.I., W.C.O., Y.F., and S.O. designed research; L.K.P., H.U., A.T.-N., R.O., W.C.O., and Y.F. performed research; L.K.P., H.U., A.T.-N., R.O., and W.C.O. analyzed data; L.K.P., H.U., W.C.O., Y.F., and S.O. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research 20H00481 (to S.O.) Grant-in-Aid for Early-Career Scientists 18K14842 (to L.K.P.), the Japan Agency for Medical Research and Development Grants JP20gm1310003 and JP20gm5010003 (to S.O.), and the UTokyo Center for Integrative Science of Human Behavior (S.O.).

Funding

  • Japan Society for the Promotion of Science (JSPS)

    20H00481; 18K14842
  • Japan Agency for Medical Research and Development (AMED)

    JP20gm1310003; JP20gm5010003
  • UTokyo Center for Integrative Science of Human Behaviour (CiSHuB)

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  • previous version (October 27, 2020).

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Geometry and the Organizational Principle of Spine Synapses along a Dendrite
Laxmi Kumar Parajuli, Hidetoshi Urakubo, Ai Takahashi-Nakazato, Roberto Ogelman, Hirohide Iwasaki, Masato Koike, Hyung-Bae Kwon, Shin Ishii, Won Chan Oh, Yugo Fukazawa, Shigeo Okabe
eNeuro 27 October 2020, 7 (6) ENEURO.0248-20.2020; DOI: 10.1523/ENEURO.0248-20.2020

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Geometry and the Organizational Principle of Spine Synapses along a Dendrite
Laxmi Kumar Parajuli, Hidetoshi Urakubo, Ai Takahashi-Nakazato, Roberto Ogelman, Hirohide Iwasaki, Masato Koike, Hyung-Bae Kwon, Shin Ishii, Won Chan Oh, Yugo Fukazawa, Shigeo Okabe
eNeuro 27 October 2020, 7 (6) ENEURO.0248-20.2020; DOI: 10.1523/ENEURO.0248-20.2020
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Keywords

  • dendritic spine
  • electron microscopy
  • FIB/SEM
  • glutamate uncaging
  • postsynaptic density
  • simulation

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