Article Figures & Data
Figures
- Figure 1.
Labeled schematic of the OV system.
- Figure 2.
Wiring diagram for OV apparatus.
- Figure 3.
Custom PCB design to facilitate building the OV apparatus.
- Figure 4.
Visual schematic outlining the CPP paradigm. Figure made using BioRender.
- Figure 5.
Plasma concentrations for nicotine (left) and cotinine (right) at 10 or 120 min after termination of a 10-min total exposure session during which vapor was delivered for one of 2, 4, or 8 min. Data represented as mean ± standard error of the mean (SEM); #p < 0.0001 120- versus 10-min time point; *p < 0.05 dose trend.
- Figure 6.
Nicotine vapor CPP in adult and adolescent (PND30–PND39) rats; *p < 0.0125 postconditioned versus preconditioned (Bonferroni corrected). Data represented as mean ± SEM.
Tables
Component Quantity Price Source of materials Arduino Uno (with cable) 1 $12.99 www.amazon.ca JUUL starter pack 2 $129.98 www.juul.ca H-bridge motor controller 1 $1.89 www.amazon.ca DC vacuum motor 2 $45.98 www.amazon.ca Solderless breadboard 1 $3.49 www.amazon.ca AC/DC converter (with power jack) 1 $13.49 www.amazon.ca Jumper cables 120 $6.98 www.amazon.ca 8-mm silicone tubing 1 (3 feet) $9.09 www.amazon.ca Heat shrink tubing pack 1 $6.99 www.amazon.ca Allentown mouse cages 2 - In-Lab 3D-printed nozzles 2 - In-Lab PCB (optional), replaces H-bridge, breadboard, and jumper cables 1 - - Total $230.88 (CAD) Step Instructions 1 Download the code file from https://www.khokharlab.com/open-source-file-downloads 2 Print the nozzles from https://www.khokharlab.com/open-source-file-downloads using a 3D printer 3 Attach the H-bridge to the breadboard 4 Wire the Arduino to the system with six “logic” cables, a power cable, and a ground 5 Connect the two terminals of each motor to the corresponding H-bridge pins (Fig. 2) 6 Insert two cables into the DC power jack (one for ground and one to power the motors) 7 Secure a charged and filled JUUL e-cigarette to each of the two motors with heat shrink 8 On the “out” ports of each motor, tightly heat shrink one end of an 8mm plastic tubes onto it 9 Heat shrink the 3D-printed nozzles onto the opposite ends of each 8-mm plastic tube and insert the nozzles into the drilled holes on the ends of each of the Allentown mouse cages 10 Plug the Arduino board into your computer and using the Arduino IDE, upload the appropriate code onto the board 11 Plug both the Arduino and the adjustable voltage supply into a receptacle. The cable to the Arduino will automatically supply the desired voltage to the board 12 Adjust the voltage supply to 6 V, and vapor clouds will appear at the specified intervals Data structure Type of test Power a Normal Two-way ANOVA 0.217 b Normal Two-way ANOVA 0.134 c Normal Two-way ANOVA 0.198 d Normal Two-way ANOVA 0.999 e Normal Two-way ANOVA 0.577 f Normal Two-way ANOVA 0.573 g Normal Linear contrast 0.713 h Normal One-sample t test 0.866 i Normal One-sample t test 0.996 j Normal One-sample t test 1.000 k Normal Two-way ANOVA 0.864 l Normal Two-way ANOVA 0.969 m Normal Two-way ANOVA 0.337 n Normal Linear contrast 0.935 o Normal One-sample t test 0.956 p Normal Two-way ANOVA 0.777 q Normal Two-way ANOVA 0.176 r Normal Two-way ANOVA 0.051
Movies
- Movie 1.
Animated build instructions.
- Movie 2.
Video of device in operation.
Extended Data 1
Arduino code for operating the OV’s vacuum pumps. Download Extended Data 1, PDF file.
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