Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT

User menu

Search

  • Advanced search
eNeuro

eNeuro

Advanced Search

 

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT
PreviousNext
Research ArticleOpen Source Tools and Methods, Novel Tools and Methods

OpenVape: An Open-Source E-Cigarette Vapor Exposure Device for Rodents

Jude A. Frie, Jacob Underhill, Bin Zhao, Giordano de Guglielmo, Rachel F. Tyndale and Jibran Y. Khokhar
eNeuro 28 August 2020, 7 (5) ENEURO.0279-20.2020; DOI: https://doi.org/10.1523/ENEURO.0279-20.2020
Jude A. Frie
1Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jacob Underhill
1Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bin Zhao
2Departments of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada, M5S 1A8
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Giordano de Guglielmo
3Department of Psychiatry, University of California, San Diego, San Diego, CA, 94720
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rachel F. Tyndale
4Departments of Pharmacology and Toxicology, and Psychiatry, University of Toronto, Ontario Canada, M5S 1A8, Centre for Addiction and Mental Health, Toronto, Ontario, Canada M6J 1H4
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jibran Y. Khokhar
1Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jibran Y. Khokhar
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Movies
  • Extended Data
  • Figure1
    • Download figure
    • Open in new tab
    • Download powerpoint
  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    Labeled schematic of the OV system.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    Wiring diagram for OV apparatus.

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    Custom PCB design to facilitate building the OV apparatus.

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    Visual schematic outlining the CPP paradigm. Figure made using BioRender.

  • Figure 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 5.

    Plasma concentrations for nicotine (left) and cotinine (right) at 10 or 120 min after termination of a 10-min total exposure session during which vapor was delivered for one of 2, 4, or 8 min. Data represented as mean ± standard error of the mean (SEM); #p < 0.0001 120- versus 10-min time point; *p < 0.05 dose trend.

  • Figure 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 6.

    Nicotine vapor CPP in adult and adolescent (PND30–PND39) rats; *p < 0.0125 postconditioned versus preconditioned (Bonferroni corrected). Data represented as mean ± SEM.

Tables

  • Figures
  • Movies
  • Extended Data
    • View popup
    Table 1

    Bill of materials

    ComponentQuantityPriceSource of materials
    Arduino Uno (with cable)1$12.99 www.amazon.ca
    JUUL starter pack2$129.98 www.juul.ca
    H-bridge motor controller1$1.89 www.amazon.ca
    DC vacuum motor2$45.98 www.amazon.ca
    Solderless breadboard1$3.49 www.amazon.ca
    AC/DC converter (with power jack)1$13.49 www.amazon.ca
    Jumper cables120$6.98 www.amazon.ca
    8-mm silicone tubing1 (3 feet)$9.09 www.amazon.ca
    Heat shrink tubing pack1$6.99 www.amazon.ca
    Allentown mouse cages2-In-Lab
    3D-printed nozzles2-In-Lab
    PCB (optional), replaces H-bridge, breadboard, and jumper cables1--
    Total$230.88 (CAD)
    • View popup
    Table 2

    Build instructions

    StepInstructions
    1Download the code file from https://www.khokharlab.com/open-source-file-downloads
    2Print the nozzles from https://www.khokharlab.com/open-source-file-downloads using a 3D printer
    3Attach the H-bridge to the breadboard
    4Wire the Arduino to the system with six “logic” cables, a power cable, and a ground
    5Connect the two terminals of each motor to the corresponding H-bridge pins (Fig. 2)
    6Insert two cables into the DC power jack (one for ground and one to power the motors)
    7Secure a charged and filled JUUL e-cigarette to each of the two motors with heat shrink
    8On the “out” ports of each motor, tightly heat shrink one end of an 8mm plastic tubes onto it
    9Heat shrink the 3D-printed nozzles onto the opposite ends of each 8-mm plastic tube and insert the nozzles into the drilled holes on the ends of each of the Allentown mouse cages
    10Plug the Arduino board into your computer and using the Arduino IDE, upload the appropriate code onto the board
    11Plug both the Arduino and the adjustable voltage supply into a receptacle. The cable to the Arduino will automatically supply the desired voltage to the board
    12Adjust the voltage supply to 6 V, and vapor clouds will appear at the specified intervals
    • View popup
    Table 3

    Statistical table

    Data structureType of testPower
    aNormalTwo-way ANOVA0.217
    bNormalTwo-way ANOVA0.134
    cNormalTwo-way ANOVA0.198
    dNormalTwo-way ANOVA0.999
    eNormalTwo-way ANOVA0.577
    fNormalTwo-way ANOVA0.573
    gNormalLinear contrast0.713
    hNormalOne-sample t test0.866
    iNormalOne-sample t test0.996
    jNormalOne-sample t test1.000
    kNormalTwo-way ANOVA0.864
    lNormalTwo-way ANOVA0.969
    mNormalTwo-way ANOVA0.337
    nNormalLinear contrast0.935
    oNormalOne-sample t test0.956
    pNormalTwo-way ANOVA0.777
    qNormalTwo-way ANOVA0.176
    rNormalTwo-way ANOVA0.051

Movies

  • Figures
  • Tables
  • Extended Data
  • Movie 1.

    Animated build instructions.

  • Movie 2.

    Video of device in operation.

Extended Data

  • Figures
  • Tables
  • Movies
  • Extended Data 1

    Arduino code for operating the OV’s vacuum pumps. Download Extended Data 1, PDF file.

Back to top

In this issue

eneuro: 7 (5)
eNeuro
Vol. 7, Issue 5
September/October 2020
  • Table of Contents
  • Index by author
Email

Thank you for sharing this eNeuro article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
OpenVape: An Open-Source E-Cigarette Vapor Exposure Device for Rodents
(Your Name) has forwarded a page to you from eNeuro
(Your Name) thought you would be interested in this article in eNeuro.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
OpenVape: An Open-Source E-Cigarette Vapor Exposure Device for Rodents
Jude A. Frie, Jacob Underhill, Bin Zhao, Giordano de Guglielmo, Rachel F. Tyndale, Jibran Y. Khokhar
eNeuro 28 August 2020, 7 (5) ENEURO.0279-20.2020; DOI: 10.1523/ENEURO.0279-20.2020

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Share
OpenVape: An Open-Source E-Cigarette Vapor Exposure Device for Rodents
Jude A. Frie, Jacob Underhill, Bin Zhao, Giordano de Guglielmo, Rachel F. Tyndale, Jibran Y. Khokhar
eNeuro 28 August 2020, 7 (5) ENEURO.0279-20.2020; DOI: 10.1523/ENEURO.0279-20.2020
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Visual Abstract
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
    • Synthesis
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • adolescent
  • e-cigarette
  • JUUL
  • nicotine
  • reward
  • vaping

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

Open Source Tools and Methods

  • Development of an Open Face Home Cage Running Wheel for Testing Activity-Based Anorexia and Other Applications
  • Simple and Efficient 3D-Printed Superfusion Chamber for Electrophysiological and Neuroimaging Recordings In Vivo
  • Decreased Dorsomedial Striatum Direct Pathway Neuronal Activity Is Required for Learned Motor Coordination
Show more Open Source Tools and Methods

Novel Tools and Methods

  • Development of an Open Face Home Cage Running Wheel for Testing Activity-Based Anorexia and Other Applications
  • Simple and Efficient 3D-Printed Superfusion Chamber for Electrophysiological and Neuroimaging Recordings In Vivo
  • Decreased Dorsomedial Striatum Direct Pathway Neuronal Activity Is Required for Learned Motor Coordination
Show more Novel Tools and Methods

Subjects

  • Novel Tools and Methods
  • Open Source Tools and Methods

  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Latest Articles
  • Issue Archive
  • Blog
  • Browse by Topic

Information

  • For Authors
  • For the Media

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
  • Feedback
(eNeuro logo)
(SfN logo)

Copyright © 2023 by the Society for Neuroscience.
eNeuro eISSN: 2373-2822

The ideas and opinions expressed in eNeuro do not necessarily reflect those of SfN or the eNeuro Editorial Board. Publication of an advertisement or other product mention in eNeuro should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in eNeuro.