Upregulation of nAChRs and Changes in Excitability on VTA Dopamine and GABA Neurons Correlates to Changes in Nicotine-Reward-Related Behavior

α4-mCherryα6-GFP mice reveal multiple subtypes of nAChRs on VTA pDA neurons. A, Schematic of target mouse brain region (bottom) and sample 10× image of a mouse coronal brain slice at approximately bregma −3.1 mm (top, no immunofluorescence was used here). Scale bar: 250 μm. B, Sample images of control and nicotine-treated VTA DA neurons, set to the same intensity scale, from α4-mCherryα6-GFP mice used in a CPP assay. Scale bar: 10 μm. C, Male and female mice were administered intraperitoneal injections of saline or 0.5 mg/kg nicotine in a CPP assay [n = 14 (8 males and 6 females) and 25 (14 males and 11 females) for saline and nicotine, respectively]. D₁–D₃, RID of α4α6*, α4*, and α6* nAChRs in saline-treated and nicotine-treated mice (from CPP assays). Individual dots represent the RID of individual mice [n = 11 (6 males and 5 females) and 19 (12 males and 7 females) for saline and nicotine, respectively]. For each mouse, 41–71 putative _LVTA DA neurons were imaged. E₁, Representative image of a putative VTA DA neuron in a brain slice (bregma, −3.1) from an α6-GFP mouse (scale bars: 20 μm). E₂, Representative voltage-clamp recording of putative _LVTA DA neurons during a 10-s puff of 300 and 500 nm nicotine. Blue bar indicates duration of nicotine puff and red dotted line represents baseline before nicotine puff. All data are mean ± SEM; *p < 0.05, **p < 0.05, ***p < 0.005; unpaired, two-tailed t test. Exact p values are given in Results. Figure Contributions: Austin T. Akers, Zachary J. Baumgard, Skylar Y. Cooper, Gabriella P. Casinelli, Alicia J. Avelar, and Brandon J. Henderson performed the experiments and analyzed the data.

Upregulation of α4α6* and α4* nAChRs in VTA pDA neurons correlates with nicotine reward-related behavior. A₁, C₁, Representative merged images of _LVTA DA neurons in a α4-mCherryα6-GFP brain slice. Scale bar, 10 μm. In nicotine-treated mice, changes in nAChR RID was correlated to CPP score for α4α6* nAChRs, α4* nAChRs, and α6* nAChRs for male (A₂, A₃, A₄) and female (B₁, B₂, B₃) mice. Linear fits (red line) with 95% confidence intervals (dotted red lines). In saline-treated mice, changes in nAChR RID was correlated to CPP score for α4α6* nAChRs (C₂), α4* nAChRs (C₃) and α6* nAChRs (C₄). Linear fits (red line) with 95% confidence intervals (dotted red lines) were applied using Graphpad Prism software. Nicotine correlations used 7 male and 8 female α4-mCherryα6-GFP mice and the saline correlations used 5 α4-mCherryα6-GFP mice (3 males and 2 females). For each mouse, 36–71 neurons were imaged.Figure Contributions: Austin T. Akers, Zachary J. Baumgard, and Brandon J. Henderson performed the experiments and analyzed the data.

Upregulation of α4* nAChRs on SNr and VTA putative GABAergic neurons may not correlate with nicotine reward-related behavior. A₁, B₁, and C₁, Representative images of neurons in the _LVTA (A₁), SNr (B₁), or dentate gyrus (C) in a α4-mCherryα6-GFP brain slice. Scale bar, 10 μm (A₁ and B₁), 250 μm (C₁), and 25 μm (C₂, C₃). A₂, B₂, RID of saline- and nicotine-treated mice from CPP assays for SNr GABA neurons or VTA GABA neurons. In A₂ and B₂ the n of individual male and female mice are indicated by individual dots. In nicotine-treated mice, changes in nAChR RID was correlated to CPP score for α4* nAChRs in SNr GABA neurons or VTA GABA neurons. In A₃, A₄, B₃, and B₄ the n of individual male and female mice are indicated by individual dots. D₁, RID/area of saline- and nicotine-treated mice from CPP assays for dentate gyrus (n = 6, 3 male and 3 female). D₂, Changes in nAChR RID/area was correlated to CPP score for α4* nAChRs in the dentate gyrus. Linear fits (red line) with 95% confidence intervals (dotted red lines) were applied using Graphpad Prism software. Data is Mean ± SEM; *p = 0.05, **p = 0.01; unpaired, two-tailed t test. Figure Contribution: Austin T. Akers, Zachary J. Baumgard, Skylar Y. Cooper, Alicia J. Avelar, and Brandon J. Henderson performed the experiments and analyzed the data.

Decreases in VTA pDA neuron firing frequency correlates with reward-related behavior. A, Schematic of target neurons within the _LVTA (target bregma, −3.1). B₁, B₂, Representative images of VTA pDA neurons in DIC (B₁) and GFP (B₂) imaging modes. Scale bar: 20 μm. Mice used in CPP assays (C) were used to measure firing frequency of pDA neurons in the VTA (D, E). D, Representative cell-attached recordings of VTA pDA neuron baseline firing frequency. E, Mean VTA pDA neuron firing frequency in mice treated with saline or nicotine in CPP assays. Data are mean ± SEM, dots represent data from individual mice (n = 8–9 per condition). F, Reward-related behavior (CPP Score) was correlated to baseline firing frequency of _LVTA pDA neurons; *p < 0.05, **p < 0.01; unpaired t test. Exact p values are given in text. Figure Contributions: Brandon J. Henderson performed the experiments and analyzed the data.