Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT

User menu

Search

  • Advanced search
eNeuro

eNeuro

Advanced Search

 

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT
PreviousNext
Research ArticleResearch Article: New Research, Sensory and Motor Systems

Controlling Horizontal Cell-Mediated Lateral Inhibition in Transgenic Zebrafish Retina with Chemogenetic Tools

Billie Beckwith-Cohen, Lars C. Holzhausen, Scott Nawy and Richard H. Kramer
eNeuro 15 October 2020, 7 (5) ENEURO.0022-20.2020; DOI: https://doi.org/10.1523/ENEURO.0022-20.2020
Billie Beckwith-Cohen
1Vision Science Graduate Program, University of California, Berkeley, School of Optometry, Berkeley, CA 94720
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Billie Beckwith-Cohen
Lars C. Holzhausen
2Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Scott Nawy
2Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Scott Nawy
Richard H. Kramer
1Vision Science Graduate Program, University of California, Berkeley, School of Optometry, Berkeley, CA 94720
2Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Richard H. Kramer
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    Strategy for chemogenetic manipulation of HC membrane potential. A, A bicistronic construct containing the molluscan FaNaC from H. aspersa and the red fluorescent protein mCherry, both under the control of the Cx55.5 promoter, were transgenically expressed in HCs of zebrafish causing red fluorescence specific to the HC layer. mCherry exhibits intrinsic fluorescence seen in confocal imaging of a fixed retinal section (left) and in two-photon imaging of a fresh retinal flat mount (right). Puncta with saturating expression of mCherry likely represent protein aggregates within the Golgi apparatus of HCs. A diagram illustrates how binding of the agonist FMRFamide causes Na+ influx thereby depolarizing the membrane potential. B, A bicistronic construct containing the PSAM-GlyR and the green fluorescent protein eGFP, both under the control of the Cx55.5 promoter, were transgenically expressed in HCs of zebrafish causing green fluorescence specific to the HC layer. eGFP was immunolabeled with Alexa Fluor 488 for confocal imaging of a fixed retinal section (left) and its intrinsic fluorescence is seen in two-photon imaging of a fresh retinal flat mount (right). A diagram illustrates how binding of the designer agonist PSEM89S causes Cl– influx thereby clamping the membrane potential to ECl. Nuclei are stained with DAPI (blue). Scale bar: 20 μm.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    Monitoring changes in membrane potential elicited by FMRFamide and PSEM89S. A, Voltage response to a 100-ms puff of PSEM89S (500 μm) in an isolated HC recorded with the gramicidin perforated patch configuration. A puff was delivered at the time indicated by the arrow. Although the concentration of PSEM89S was 500 μm in the pipette, the concentration of the drug is estimated to be diluted ∼50-fold at the cell (Firestein and Werblin, 1989), positioned ∼100 μm from the puffer pipette. B, Traces from three different HCs showing responses to 100-ms puffs of GABA (1 mm), chosen to highlight the variability of responses to GABA that were observed between cells. C, Response to 100-ms application of muscimol (100 μm), a GABAA receptor agonist. Responses were uniformly hyperpolarizing in all cells tested. D, Summary data showing the resting membrane potential, and the membrane potential at the peak of the response to PSEM89S. Large bolded symbols are the mean ± SEM for each condition (n = 6). E, Summary of the change in membrane potential evoked by puffs of GABA or muscimol. Small closed symbols are the responses of individual cells to GABA (n = 11). Open symbols are the responses to muscimol (n = 7). Open boxes are the mean ± SEM for hyperpolarizing responses, and the shaded box is the mean ± SEM for depolarizing responses. The mean amplitude of the responses evoked by muscimol was not significantly different from the amplitude of hyperpolarization evoked by GABA (p = 0.53, rank-sum test). F, Summary data for bath application of FMRFamide (30 μm). Large bolded symbols are the mean ± SEM for each condition (n = 7).

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    Chemogenetic activation of HCs alters vesicular release of FM1-43 from cone terminals. A, Schematic representation of the mechanisms of negative (–) and positive (+) feedback of HCs onto cone photoreceptors (PR). VGCCs are voltage-gated Ca2+ channels, green circles are FM1-43-filled synaptic vesicles, orange dots are glutamate molecules in the synaptic cleft, AMPARs are AMPA receptors. Negative feedback is mediated by changes in the membrane potential of HCs, whereas positive feedback is mediated by increased intracellular Ca2+ in HCs, owing to influx of Ca2+ through Ca2+-permeant AMPA receptors. ΔCai is the change in intracellular Ca2+; ΔVM is the change in HC membrane potential. B, Two-photon scanned images of the photoreceptor terminal layer in FaNaC zebrafish retinas. Retinas were pretreated with FM1-43 to load recycling synaptic vesicles and then treated for 20 min with the indicated receptor agonist. AMPA (20 μm) increased the rate of dye loss (destaining) and FMRFamide (10 μm) decreased destaining, but the AMPA-elicited increase was dominant when the two agonists were applied together. C, D, Time course of FM1-43 destaining in FaNaC fish. Without added agonists, cone terminals released FM1-43 at 0.87 ± 0.08% per minute (n = 10 retinas). Adding AMPA accelerated destaining rate (1.89 ± 0.13%, n = 9 retinas, p < 1 × 10−5). Adding FMRFamide (10 μm) decelerated destaining (0.54 ± 0.07% n = 9 retinas, p = 0.001). Adding AMPA and FMRFamide together resulted in a significant accelerated destaining rate from dark (p = 0.002), which was not significantly different from that with AMPA alone (1.96 ± 0.25%, n = 4, p = 0.88). Statistical differences were determined with one-way ANOVA (F(3,23) = 27.4, p = 8.9 × 10−8). E, F, Time course of FM1-43 destaining in PSAM fish. Adding PSEM89S (30 μm) accelerated destaining (0.83 ± 0.19%, n = 9 retinas, p = 0.044). Adding AMPA accelerated destaining (2.19 ± 0.19%, n = 5 retinas, p = 0.0002). Adding PSEM89S and AMPA together resulted in a destaining rate not significantly different from that with AMPA alone (2.05 ± 0.35%, n = 5, p = 0.82). Analysis was performed with one-way ANOVA (F(3,24) = 14.69, p = 1.2 × 10−5). For D and F open circles represent individual data points. Open boxes represent the interquartile range with whiskers. The mean is marked by x and the median by a line.

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    Chemogenetic manipulation of HCs modulates the bipolar cell light response. Application of FMRFamide had no effect on the ERG of WT zebrafish (A), but decreased the maximal b-wave amplitude in FaNaC zebrafish (B), reaching a maximum ∼40% decrease in the b-wave (C). Application of the buffer HEPES (pH 7.35) cancelled the effects of FMRFamide (C). FMRFamide response was dose dependent having an EC50 of 4.25 μm (D). Application of the agonist PSEM89S (30 μm) also caused a decrease in the maximal b-wave amplitude, but to a lesser degree (E, F).

  • Figure 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 5.

    Expression of GCaMP6f in chemogenetic zebrafish lines. A, Ex vivo retina of adult HuC-GCaMP6f zebrafish shows eGFP fluorescence in retinal flat mounts using two-photon imaging. B, Light response is easily measured in RGCs before (left) and after (right) a light flash. C, In vivo confocal imaging of eGFP in 1 dpf PTU-treated zebrafish larvae of HuC-GCaMP6f and FaNaC crossed transgenic fish. The optic nerve (ON) and the developing retina (RGC) show strong eGFP fluorescence. D, In vivo imaging of the same line of fish imaged in C showing that FaNaC-mCherry is easily visualized in HCs at 2 dpf zebrafish larvae. Some red autofluorescence is generated by scattered pigment cells on the ocular surface. RGC, retinal ganglion cell layer; IPL, inner plexiform layer; BC, bipolar cell layer; HC, horizontal cell layer.

  • Figure 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 6.

    Chemogenetic manipulation of HCs alters lateral inhibition in downstream RGCs. A, Light responses were measured in HuC::GCaMP6f fish with three light stimuli, delivered at six different spot diameters. Calcium transients were recorded using fluorescence imaging. B, WT RGCs responded with maximum change in fluorescence to a 500-μm spot of light, which decreased at 1000 μm, supporting lateral inhibition. This effect was blocked by HEPES, and was unchanged by applying 10 μm FMRFamide. C, Application of FMRFamide on FaNaC retinas perturbed normal RGC center surround response, (D) as did application of PSEM89S on PSAM- GlyR retinas. E, The LIR in various conditions shows that application of the cognate agonists to FaNaC and PSAM retina significantly disrupts lateral inhibition. Open circles represent individual data points. Open boxes represent the interquartile range with whiskers. The mean is marked by x and the median by a line.

Back to top

In this issue

eneuro: 7 (5)
eNeuro
Vol. 7, Issue 5
September/October 2020
  • Table of Contents
  • Index by author
Email

Thank you for sharing this eNeuro article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Controlling Horizontal Cell-Mediated Lateral Inhibition in Transgenic Zebrafish Retina with Chemogenetic Tools
(Your Name) has forwarded a page to you from eNeuro
(Your Name) thought you would be interested in this article in eNeuro.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Controlling Horizontal Cell-Mediated Lateral Inhibition in Transgenic Zebrafish Retina with Chemogenetic Tools
Billie Beckwith-Cohen, Lars C. Holzhausen, Scott Nawy, Richard H. Kramer
eNeuro 15 October 2020, 7 (5) ENEURO.0022-20.2020; DOI: 10.1523/ENEURO.0022-20.2020

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Share
Controlling Horizontal Cell-Mediated Lateral Inhibition in Transgenic Zebrafish Retina with Chemogenetic Tools
Billie Beckwith-Cohen, Lars C. Holzhausen, Scott Nawy, Richard H. Kramer
eNeuro 15 October 2020, 7 (5) ENEURO.0022-20.2020; DOI: 10.1523/ENEURO.0022-20.2020
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
    • Synthesis
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • feedback inhibition
  • fluorescent protein
  • horizontal cell
  • lateral inhibition
  • photoreceptor
  • retina

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

Research Article: New Research

  • Opponent Learning with Different Representations in the Cortico-Basal Ganglia Circuits
  • Cardiac and Gastric Interoceptive Awareness Have Distinct Neural Substrates
  • Nonspiking Interneurons in the Drosophila Antennal Lobe Exhibit Spatially Restricted Activity
Show more Research Article: New Research

Sensory and Motor Systems

  • Pregabalin silences oxaliplatin-activated sensory neurons to relieve cold allodynia
  • Supramodal representation of the sense of body ownership in the human parieto-premotor and extrastriate cortices
  • Nonspiking Interneurons in the Drosophila Antennal Lobe Exhibit Spatially Restricted Activity
Show more Sensory and Motor Systems

Subjects

  • Sensory and Motor Systems

  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Latest Articles
  • Issue Archive
  • Blog
  • Browse by Topic

Information

  • For Authors
  • For the Media

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
  • Feedback
(eNeuro logo)
(SfN logo)

Copyright © 2023 by the Society for Neuroscience.
eNeuro eISSN: 2373-2822

The ideas and opinions expressed in eNeuro do not necessarily reflect those of SfN or the eNeuro Editorial Board. Publication of an advertisement or other product mention in eNeuro should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in eNeuro.