Extended Data Figure 1-2
Bout Features. A, Bout feature distributions during the day (light blue) and the night (dark blue). For the probability curves, each animal’s data were fit with a pdf. Shown is a mean pdf (bold line) and SD (shaded surround) with a log scale on the x-axis. For the scatter plots, each larva’s mean value across the days or nights (5–6 dpf) is shown as a light blue (day) or dark blue circle (night). An orange cross marks each population’s mean. Of the pdfs, only the mean day and night active bout total and inactive bout length pdfs were consistently significantly different across three independent experiments (p < 0.01; two-sample Kolmogorov–Smirnov test); n = 124 wild-type larvae. B, Melatonin bout feature means. A mean was taken per animal per feature, and day or night (6 dpf). Shown is a population mean and SEM during the day (white background) and the night (grey background). Control, DMSO; n = 24 controls then n = 12 per dose. C, PTZ bout feature means, as in B. Control, H2O; n = 24 controls then n = 10 (2.5 mM), n = 9 (5 mM), and n = 9 (7.5 mM). D, hcrtr bout feature means as in B, for days (white background) and nights (grey background) 5–6 post fertilization. hcrtr-/- mutants had significantly lower mean values compared to both hcrtr+/+ and hcrtr-/+ for the following active bout features: length, SD and total (p < 0.05 for all comparisons, Dunn–Sidak corrected four-way ANOVA, adjusted for the following factors: day/night, development, and experimental repeat). No features differed significantly between hcrtr-/+ and hcrtr+/+; n = 39, 102, and 39; for WT, hcrtr+/+; Het, hcrtr-/+; and Hom, hcrtr-/-, respectively. Download Figure 1-2, TIF file.