Figure 7. Effects of long-term (4 d) sCaLTs inhibition on OL differentiation and growth in culture. A, Representative images of cells exposed to different calcium inhibitors. One day after plating, cells were treated with vehicle or inhibitors for 4 d. Cells were fixed at DIV5 and stained for α-tubulin (green) and OL markers (Olig2, O4, or MBP; red). Scale bar: 50 μm. B, Bar graphs showing the percentage of cells positive for each OL marker after respective drug treatment. C, Bar graphs showing the quantification of the total number of cells for each drug condition. D, E, Bar graphs showing D values (fractal dimension) and the total arbor size of OLs after each treatment. n = number of cells from at least three independent experiments; *statistical difference (p < 0.05) compared with the corresponding vehicle or control group (one-way ANOVA with Tukey’s HSD test). Error bars represent the standard error. Quantification of positive markers: Olig2+ (df = 3, F = 48.610, p = 9.841E-18; Tukey’s HSD test: Ctrl vs SKF, p = 0.000014; Ctrl vs Tg, p = 5.7843E-13; Ctrl vs Nife, p = 0.006978), O4+ (df = 3, F = 77.082, p = 1.5532E-25; Tukey’s HSD test: Ctrl vs SKF, p = 0.048013; Ctrl vs Tg, p = 4.8717E-13; Ctrl vs Nife, p = 0.396643), MBP+ (df = 3, F = 80.817, p = 2.0443E-24; Tukey’s HSD test: Ctrl vs SKF, p =1.8915E-8; Ctrl vs Tg, p = 5.3879E-13; Ctrl vs Nife, p = 0.000056), quantification of fractal dimension (df = 3, F = 59.285, p = 1.3288E-27; Tukey’s HSD test: Ctrl vs SKF, p = 4.5963E-13; Ctrl vs Tg, p = 4.5952E-13; Ctrl vs Nife, p = 0.064), quantification of surface area of OLs (df = 3, F = 45.033, p = 2.6408E-22; Tukey’s HSD test: Ctrl vs SKF, p = 1.0261E-8; Ctrl vs Tg, p = 3.9668E-13; Ctrl vs Nife, p = 0.786579).