Article Figures & Data
Figures
- Figure 1.
WIN activates an astrocytic Ca2+ signaling pathway. A, B, Representative GCaMP6 images from slices treated with (A) DMSO or (B) WIN. C, D, Representative traces showing the change in fluorescence from all regions measured (gray) and average response (black) from the slice depicted above. Black arrow indicates the beginning of treatment. Scale bar: 50 μm; images taken at 40× and the insets magnified 120×. (Adapted from Figure 3 in Hablitz et al., 2020.)
- Figure 2.
Neurons use endocannabinoid signaling to activate astrocyte Ca2+ signaling pathways. A, Representative bright-field image from a single slice in which the white arrow indicates the placement of the microelectrode tip. B, Representative intensity changes before and after depolarization of an SCN neuron (indicated by black bar) before (left, control) and during (right) AM251 treatment. White scale bar: 50 μm; images taken at 40×. (Adapted from Figure 5 in Hablitz et al., 2020.)
- Figure 3.
Daytime application of either WIN or adenosine phase advanced the molecular clock. A, Representative bioluminescence recordings from two separate slices, one control (black), the other WIN treated (red), over 8 d. Treatment artifact is indicated by the black rectangle. B, Box plot of phase shifts of all the cultures after treatment with either control (white) or WIN (red), DPCPX (light gray), or WIN+DPCPX (dark gray; *p < 0.05). C, Representative bioluminescence recordings from two separate slices, one control (black) and the other adenosine treated (blue), over 7 d. Treatment artifact is indicated by the black rectangle. D, Box plot of phase shifts of SCN slice cultures after treatment with either control (black) or adenosine (blue; *p < 0.05). (Adapted from Figure 10 in Hablitz et al., 2020.)
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