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Research ArticleOpen Source Tools and Methods, Novel Tools and Methods

An Open Source Automated Bar Test for Measuring Catalepsy in Rats

Karling R. Luciani, Jude A. Frie and Jibran Y. Khokhar
eNeuro 20 March 2020, 7 (3) ENEURO.0488-19.2020; https://doi.org/10.1523/ENEURO.0488-19.2020
Karling R. Luciani
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G2W1, Canada
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Jude A. Frie
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G2W1, Canada
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Jibran Y. Khokhar
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G2W1, Canada
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    Figure 1.

    3D printed, automated catalepsy bar test (A), diagram of a rat in the bar test (B), and circuit schematic for automated bar test assembly (C).

  • Figure 2.
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    Figure 2.

    Mean time spent on catalepsy bar in seconds as measured by the apparatus in adult male rats. Error bars represent SEM; *p < 0.0001 compared with vehicle; #p < 0.01 compared with 1 mg/kg dose. Raw data included as Extended Data Figure 2-1.

Tables

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    Table 1

    Bill of materials

    QuantityComponent nameWebsitePrice (United States)
    1Adafruit Metro Mini 328 https://www.adafruit.com/product/2590 12.50
    1Keyestudio 1602 LCD IIC/I2C/TWI Display 16x2 https://www.amazon.com/Keyestudio-Display-Module-Arduino-raspberry/dp/B01799UUGS/ 7.99
    1IR Break Beam Sensor - 3mm https://www.adafruit.com/product/2167 1.95
    15V MicroUSB Power Supply https://www.amazon.com/Travel-Charger-Adapter-Samsung-Galaxy/dp/B0117O020U/ref=sr_1_3?keywords=5v+micro+usb+power+supply&qid=1580226650&sr=8-3 6.79
    1Adafruit Perma-Proto Breadboard https://www.adafruit.com/product/1606 6.95
    1Jumper Wires https://www.adafruit.com/product/1957 1.95
    1Threaded Steel Rod - 0.5” diameter, 12” length https://www.homedepot.ca/product/paulin-1-2-13x12-inch-thread-rod-plated-/1000797593 1.64
    110 kΩ resistor https://www.adafruit.com/product/2784 0.75
    1MicroSD Card Adaptor https://www.amazon.com/HiLetgo-Adater-Interface-Conversion-Arduino/dp/B07BJ2P6X6/ref=sr_1_2?keywords=sd+card+arduino&qid=1580155120&s=electronics&sr=1-2 6.99
    40.5” Nuts https://www.homedepot.ca/product/paulin-1-2-13-fin-hex-nuts-gr2-unc/1000123145 1.30
    1Push Button https://www.adafruit.com/product/1119 2.50
    1PLA Filament (2/3 roll) https://www.amazon.com/Printer-Filament-SUNLU-Dimensional-Accuracy/dp/B07XG3RM58/ref=sr_1_6?keywords=pla+filament&qid=1580155473&sr=8-6 17.99(2/3 = 11.99)
    1Additional wire https://www.homedepot.ca/product/southwire-simpull-t90-copper-electrical-wire-14-solid-blue-300m/1000150577 0.42
    • Total cost = $63.72.

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  • Extended Data Figure 2-1

    A sample file from the logger with the raw data used for Figure 2. Download Figure 2-1, CSV file

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An Open Source Automated Bar Test for Measuring Catalepsy in Rats
Karling R. Luciani, Jude A. Frie, Jibran Y. Khokhar
eNeuro 20 March 2020, 7 (3) ENEURO.0488-19.2020; DOI: 10.1523/ENEURO.0488-19.2020

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An Open Source Automated Bar Test for Measuring Catalepsy in Rats
Karling R. Luciani, Jude A. Frie, Jibran Y. Khokhar
eNeuro 20 March 2020, 7 (3) ENEURO.0488-19.2020; DOI: 10.1523/ENEURO.0488-19.2020
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Keywords

  • antipsychotic
  • Automated
  • catalepsy
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