Article Figures & Data
Figures
- Figure 1.
Assembly of the RAT. A, Exploded schematic of the major parts for building RAT. B, Photograph of the parts for building rat. a, 3D-printed housing top. b, OpenMV LCD shield. c, Adafruit DS3231 RTC module. d. Push-button. e. BNC connector. f, 3D-printed housing bottom. g, Custom PCB. h, JST 2 pin connector. i, OpenMV M7 camera. j, MicroSD card with RAT code. C, Photograph of assembled RAT circuit board. D, Photograph of the assembled RAT electronics. E, Photograph of assembled RAT in 3D-printed housing.
- Figure 2.
Validation of tracking performance. A, Example images of black and white mice tracked by RAT in light or dark conditions. B, Example xy scatter track plot of data exported from RAT. C, Photograph of experimental validation setup recording the same mouse with RAT and a webcam connected to Bonsai. D, x and y positions from both RAT and Bonsai, demonstrating strong correlation in mouse position data between the two systems.
- Figure 3.
Experimental use cases for RAT. A, Demonstration of RAT tracking, showing a circadian rhythm in movement levels over 90 h. Lights were off for duration of experiments, gray bars represent the normal night cycle. B, Schematic of mouse nose-poking to obtain pellets from FED3 device. C, Example track plot for 30 min, showing locations of a mouse when he retrieved pellets from FED3. D, Perievent histogram and raster showing speed of mouse around pellet retrieval events. E, Example track plot for optogenetic real-time self-stimulation experiment. Blue squares show location when blue LED turned on to stimulate direct pathway medium spiny neurons in the striatum. F, G, Quantification of average time on each side of the chamber (n = 4 mice).
Tables
Component Number Cost per unit Total cost Source of materials OpenMV Cam M7 1 $65.00 $65.00 Openmv.io LCD shield 1 $20.00 $20.00 Openmv.io Adafruit DS3231 RTC breakout 1 $13.95 $13.95 Adafruit.com 3D-printed enclosure 1 ∼$5 ∼$5 Any printer will work Breakout PCB 1 $2.00 $2.00 Seeed.io JST right-angle connector 1 $0.95 $0.95 Karlsson Robotics
P/N PRT-09749Tactile button 1 $0.49 $0.49 Karlsson Robotics
P/N COM-10302Long break away male headers 2 $0.75 $0.75 Mouser
P/N 474-PRT-12693Right-angle BNC connector 1 $2.43 $2.43 Mouser
P/N 523-31-5431Undercut flat head screws
4–40 thread
⅝’’ length7 $0.06 $0.42 McMaster-Carr
P/N 91099A169Li-Ion battery (optional) 1 $9.95 $9.95 Adafruit
P/N 1781MicroUSB cable
(optional)1 $6.97 $6.97 Cdwg.com
Movies
- Movie 1.
RTPP_example.mp4. Video demonstrating the real-time-place-preference assay.
Extended data 1
RAT_code.zip. Code to set the clock and run the tracking algorithm on the RAT. Download Extended Data 1, ZIP file.
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