Figure 3. Time course of changes in EP and velocity sensitivities during spontaneous eye movements after axotomy and VEGF administration. A, EP sensitivities (ks) of motoneurons recorded at the indicated days after axotomy (red dots; lesion corresponds to time 0) and after axotomy followed by VEGF administration (green dots). The mean control value (solid dot, in blue) and its SD (blue bars) are shown at the left of the graph. B, Same as A, but for EV sensitivity (rs). C, Mean ± SEM of ks values for the control population (c) and at different weeks (w) after either axotomy (in red) or axotomy plus the single VEGF delivery (in green). Horizontal dashed lines represent the a alone (lower line). There were significant differences between axotomy and VEGF groups comparing per week (two-way ANOVA, p < 0.001). D, Same as C, but for rs. No significant differences were found between the mean values obtained per week after the supply of VEGF for ks (C; one-way ANOVA, p = 0.275) or rs (D; one-way ANOVA, p = 0.201). For C, D, the number of motoneurons recorded at the different weeks after treatment ranged between 12 and 40, and for axotomy between 8 and 28; n = 64 control motoneurons. E, Bar chart illustrating ks data and its mean value for in the population of motoneurons recorded in the control situation, after only axotomy and after axotomy plus VEGF administration. The asterisk indicates significant difference between the axotomy and control groups (one-way ANOVA, p < 0.001). On the contrary, the VEGF-treated group showed no significant difference with control (p = 0.437). F, Same as E, but for rs values. The axotomy group showed a significant difference in rs with respect to control (asterisk, one-way ANOVA, p < 0.001), whereas the VEGF-treated group was similar to control (p = 0.064). For E, F, the number of motoneurons analyzed in each situation was n = 64, 57, and 199 for the control, axotomy, and VEGF-treated groups, respectively.