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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Targeting Morphine-Responsive Neurons: Generation of a Knock-In Mouse Line Expressing Cre Recombinase from the Mu-Opioid Receptor Gene Locus

Julie Bailly, Natalie Del Rossi, Léonie Runtz, Jing-Jing Li, DaWoon Park, Grégory Scherrer, Arnaud Tanti, Marie-Christine Birling, Emmanuel Darcq and Brigitte L. Kieffer
eNeuro 7 May 2020, 7 (3) ENEURO.0433-19.2020; https://doi.org/10.1523/ENEURO.0433-19.2020
Julie Bailly
1Department of Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec H4H 1R3, Canada
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Natalie Del Rossi
1Department of Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec H4H 1R3, Canada
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Léonie Runtz
1Department of Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec H4H 1R3, Canada
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Jing-Jing Li
1Department of Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec H4H 1R3, Canada
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DaWoon Park
1Department of Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec H4H 1R3, Canada
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Grégory Scherrer
2Department of Cell Biology and Physiology, UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
2Department of Cell Biology and Physiology, UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
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Arnaud Tanti
1Department of Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec H4H 1R3, Canada
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Marie-Christine Birling
4CELPHEDIA, PHENOMIN, Institut Clinique de la Souris (ICS), 67404 Illkirch-Graffenstaden, France
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  • ORCID record for Marie-Christine Birling
Emmanuel Darcq
1Department of Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec H4H 1R3, Canada
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Brigitte L. Kieffer
1Department of Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec H4H 1R3, Canada
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0433-19.2020
PubMed 
32381649
Published By 
Society for Neuroscience
History 
  • Received October 17, 2019
  • Revision received March 23, 2020
  • Accepted April 9, 2020
  • Published online May 7, 2020.
Copyright & Usage 
Copyright © 2020 Bailly et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Julie Bailly1,
  2. Natalie Del Rossi1,
  3. Léonie Runtz1,
  4. Jing-Jing Li1,
  5. DaWoon Park1,
  6. Grégory Scherrer2,3,
  7. Arnaud Tanti1,
  8. Marie-Christine Birling4,
  9. Emmanuel Darcq1 and
  10. Brigitte L. Kieffer1
  1. 1Department of Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec H4H 1R3, Canada
  2. 2Department of Cell Biology and Physiology, UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
  3. 3New York Stem Cell Foundation – Robertson Investigator
  4. 4CELPHEDIA, PHENOMIN, Institut Clinique de la Souris (ICS), 67404 Illkirch-Graffenstaden, France
  1. Correspondence should be addressed to Brigitte L. Kieffer at brigitte.kieffer{at}douglas.mcgill.ca.
View Full Text

Author contributions

  1. Author contributions: J.B., G.S., M.-C.B., E.D., and B.L.K. designed research; J.B., N.D.R., L.R., J.-J.L., D.P., and A.T. performed research; J.B. and M.-C.B. contributed unpublished reagents/analytic tools; J.B., N.D.R., L.R., J.-J.L., E.D., and B.L.K. analyzed data; J.B., E.D., and B.L.K. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the National Institutes of Health (National Institute of Drug Abuse Grant DA-05010 to B.L.K.; and National Institute on Alcohol Abuse and Alcoholism Grant 16658 to B.L.K.), the Canada Fund for Innovation, and the Canada Research Chairs to E.D. and B.L.K., and by the Wallonie-Bruxelles International (to J.B.).

Funding

  • HHS | NIH | National Institute on Drug Abuse (NIDA)

    DA05010
  • HHS | NIH | National Institute on Alcohol Abuse and Alcoholism (NIAAA)

    16658

Other Version

  • You are viewing the most recent version of this article.
  • previous version (May 07, 2020).

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May 2020106063488
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Total 20263851892
Total210281213008
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Targeting Morphine-Responsive Neurons: Generation of a Knock-In Mouse Line Expressing Cre Recombinase from the Mu-Opioid Receptor Gene Locus
Julie Bailly, Natalie Del Rossi, Léonie Runtz, Jing-Jing Li, DaWoon Park, Grégory Scherrer, Arnaud Tanti, Marie-Christine Birling, Emmanuel Darcq, Brigitte L. Kieffer
eNeuro 7 May 2020, 7 (3) ENEURO.0433-19.2020; DOI: 10.1523/ENEURO.0433-19.2020

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Targeting Morphine-Responsive Neurons: Generation of a Knock-In Mouse Line Expressing Cre Recombinase from the Mu-Opioid Receptor Gene Locus
Julie Bailly, Natalie Del Rossi, Léonie Runtz, Jing-Jing Li, DaWoon Park, Grégory Scherrer, Arnaud Tanti, Marie-Christine Birling, Emmanuel Darcq, Brigitte L. Kieffer
eNeuro 7 May 2020, 7 (3) ENEURO.0433-19.2020; DOI: 10.1523/ENEURO.0433-19.2020
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Keywords

  • cell-specific gene targeting
  • Cre-loxP system
  • enkephalins
  • knock-in mice
  • morphine
  • Oprm1 gene

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