Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT

User menu

Search

  • Advanced search
eNeuro
eNeuro

Advanced Search

 

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Blog
    • Collections
    • Podcast
  • TOPICS
    • Cognition and Behavior
    • Development
    • Disorders of the Nervous System
    • History, Teaching and Public Awareness
    • Integrative Systems
    • Neuronal Excitability
    • Novel Tools and Methods
    • Sensory and Motor Systems
  • ALERTS
  • FOR AUTHORS
  • ABOUT
    • Overview
    • Editorial Board
    • For the Media
    • Privacy Policy
    • Contact Us
    • Feedback
  • SUBMIT
PreviousNext
Research ArticleResearch Article: New Research, Cognition and Behavior

The Reuniens and Rhomboid Nuclei Are Required for Acquisition of Pavlovian Trace Fear Conditioning in Rats

Yu-Ju Lin, Ruei-Jen Chiou and Chun-hui Chang
eNeuro 11 June 2020, 7 (3) ENEURO.0106-20.2020; https://doi.org/10.1523/ENEURO.0106-20.2020
Yu-Ju Lin
1Institute of Systems Neuroscience, National Tsing Hua University, Hsinchu 30013, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ruei-Jen Chiou
2Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chun-hui Chang
1Institute of Systems Neuroscience, National Tsing Hua University, Hsinchu 30013, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Chun-hui Chang
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    Histology confirmation of this study. A, An illustrative representation showing cannula placement in the ReRh. B, A Nissl-stained coronal section showing the cannula placement in the ReRh. C, A dark-field image showing diffusion of TMR-X muscimol in the ReRh.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    Re neurons were generally recruited in the encoding phase of trace and delay fear conditioning. A, Animals underwent a 2-d behavioral experiment, in which half of the animals in each group were killed after conditioning and the rest after retrieval test. B, c-Fos+ expression in Re neurons (red square). c-Fos signals were visualized with blue/black reaction products within the nucleus of activated neurons. C, The freezing behavior of TRACE, DELAY, UNPAIRED, and NoCOND groups. Freezing levels of the conditioning (n = 12 per group) and test trials (n = 6 per group) are presented in left panel, respectively. The middle panel shows the averaged freezing levels of the UNPAIRED group (gray line with SEM) and the divergent high (solid lines) or low (dashed lines) freezing levels of each animal during retrieval test. The right panel shows the average c-Fos+ count of the UNPAIRED group (gray bar with SEM) and the counts of high freezing (open circles; behaviors shown in solid lines) and low freezing (filled circles; behaviors shown in dashed lines) animals based on their behavior on trial 3 (n = 3 each). D, Group differences in c-Fos+ counts of the Re after conditioning (upper panel) and test (lower panel); *p < 0.05. NS, not significant.

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    The ReRh was critically involved during the acquisition of trace fear conditioning. A, Experimental design. Animals underwent a 2-d behavioral experiment, in which the animals received drug infusion immediately before conditioning (day 1). B, Injector tip placements of all the animals included in data analyses at levels −1.92, −2.16, −2.52, and −3.00 mm posterior relative to bregma. The gray areas marked in the atlas are the Re and ventral Re. C, The freezing behavior of V-TRACE (n = 7), M-TRACE (n = 6), V-DELAY (n = 6), and M-DELAY groups (n = 5) when injections were performed before conditioning.

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    The ReRh was not involved during the consolidation of trace fear conditioning. A, Experimental design. Animals underwent a 2-d behavioral experiment, in which the animals received drug infusion immediately after conditioning (day 1). B, Injector tip placements of all the animals included in data analyses at levels −1.92, −2.16, −2.52, and −3.00 mm posterior relative to bregma. The gray areas marked in the atlas are the Re and ventral Re. C, The freezing behavior of C-TRACE (n = 8), M-TRACE (n = 7), C-DELAY (n = 7), and M-DELAY groups (n = 7) when injections were performed after conditioning.

  • Figure 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 5.

    The ReRh was not involved during the retrieval of trace fear conditioning. A, Experimental design. Animals underwent a 2-d behavioral experiment, in which the animals received drug infusion immediately before test (day 2). B, Injector tip placements of all the animals included in data analyses at levels −1.92, −2.16, −2.52, and −3.00 mm posterior relative to bregma. The gray areas marked in the atlas are the Re and ventral Re. C, The freezing behavior of V-TRACE (n = 5), M-TRACE (n = 7), V-DELAY (n = 7), and M-DELAY groups (n = 7) when injections were performed before retrieval test.

  • Figure 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 6.

    Retrieval of trace fear acquired under ReRh inactivation reprised under ReRh inactivation. A, Experimental design. Animals underwent a 2-d behavioral experiment, in which the animals received drug infusion immediately before both conditioning and retrieval test. B, Injector tip placements of all the animals included in data analyses at levels −1.92, −2.16, −2.52, and −3.00 mm posterior relative to bregma. The gray areas marked in the atlas are the Re and ventral Re. C, The freezing behavior of SAL-SAL (n = 10), MUS-SAL (n = 9), and MUS-MUS groups (n = 9) when the injections were performed before both conditioning and retrieval test.

Back to top

In this issue

eneuro: 7 (3)
eNeuro
Vol. 7, Issue 3
Month/Month
  • Table of Contents
  • Index by author
  • Ed Board (PDF)
Email

Thank you for sharing this eNeuro article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
The Reuniens and Rhomboid Nuclei Are Required for Acquisition of Pavlovian Trace Fear Conditioning in Rats
(Your Name) has forwarded a page to you from eNeuro
(Your Name) thought you would be interested in this article in eNeuro.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
The Reuniens and Rhomboid Nuclei Are Required for Acquisition of Pavlovian Trace Fear Conditioning in Rats
Yu-Ju Lin, Ruei-Jen Chiou, Chun-hui Chang
eNeuro 11 June 2020, 7 (3) ENEURO.0106-20.2020; DOI: 10.1523/ENEURO.0106-20.2020

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Share
The Reuniens and Rhomboid Nuclei Are Required for Acquisition of Pavlovian Trace Fear Conditioning in Rats
Yu-Ju Lin, Ruei-Jen Chiou, Chun-hui Chang
eNeuro 11 June 2020, 7 (3) ENEURO.0106-20.2020; DOI: 10.1523/ENEURO.0106-20.2020
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Significance Statement
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
    • Synthesis
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • behavioral pharmacology
  • nucleus reuniens
  • rhomboid nucleus
  • trace fear conditioning

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

Research Article: New Research

  • Novel roles for the GPI-anchor cleaving enzyme, GDE2, in hippocampal synaptic morphology and function
  • Upright posture: a singular condition stabilizing sensorimotor coordination
  • Serotonergic signaling governs C. elegans sensory response to conflicting chemosensory stimuli.
Show more Research Article: New Research

Cognition and Behavior

  • Novel roles for the GPI-anchor cleaving enzyme, GDE2, in hippocampal synaptic morphology and function
  • Upright posture: a singular condition stabilizing sensorimotor coordination
  • Modulation of hippocampal sharp-wave ripples by behavioral states and body movements in head-fixed rodents
Show more Cognition and Behavior

Subjects

  • Cognition and Behavior
  • Home
  • Alerts
  • Follow SFN on BlueSky
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Latest Articles
  • Issue Archive
  • Blog
  • Browse by Topic

Information

  • For Authors
  • For the Media

About

  • About the Journal
  • Editorial Board
  • Privacy Notice
  • Contact
  • Feedback
(eNeuro logo)
(SfN logo)

Copyright © 2025 by the Society for Neuroscience.
eNeuro eISSN: 2373-2822

The ideas and opinions expressed in eNeuro do not necessarily reflect those of SfN or the eNeuro Editorial Board. Publication of an advertisement or other product mention in eNeuro should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in eNeuro.