Figure 9. Representative micrographs illustrating, on the coronal plane, the negligible expression of PK-resistant α-syn immunoreactivity at seven, nine, and 12 months postinjection in the vertical limb of the DBB (vDBB;
A
, inset shown in
A′
), and the anterior and posterior piriform cortices (aPC and pPC;
B
,
C
, respectively, insets shown in
B′
,
C′
). Spreading of α-syn aggregates. A strong PK-resistant α-syn immunoreactivity was also found at 12 months postinjection in the vDBB (
D
, inset shown in
D′
, arrow) and the anterior and posterior piriform cortices (aPC and pPC,
E
,
F,
insets shown in
E′
,
F′
; arrows and arrowheads indicate LB-like and LN-like inclusions, respectively). Scale bar: 100 μm (in
A
,
D
, for
A–C
,
D–F
) and 50 μm (
D
for
D–F
). Cresyl violet staining of the entorhinal cortex (ENT CTX), hippocampus (HPC), and piriform cortices (PC) of control (
G
,
H
,
I
, respectively) and α-syn-injected animals (
J
,
K
,
L
, respectively). Scale bar: 100 μm (in
G
for
G–L
).