Extended Data Figure 7-2
Inhibition of Akt does not affect basal neural network activity in control, p53f/+−Emx1-Cre−, cultures. A, B, Quantification of relative spontaneous spike frequency (A, left), synchrony (A, right), burst duration (B, left) and relative number of spikes per burst (B, right) from p53f/+−Emx1-Cre− cortical neuron cultures treated with DMSO, DHPG (100 μM), DMSO+MK-2206 (1 μM), or DHPG+MK-2206 at DIV14. The analysis was done by comparing “after treatment” to “before treatment” of the same cultures during the 15-min recordings (n = 5–7 independent cultures). A two-way ANOVA with Tukey’s test was used. Data are represented as mean ± SEM with *p < 0.05 and ns: non-significant. Download Figure 7-2, TIF file.