Extended Data Figure 2-3
Significance of a low scaling factor. A–I, The Shank2 confocal data for DIV14 rat cultures treated with 2 μM TTX for 48 h and sampled at 200, 400, 600, 800, 1000, 1200, 1400, 1600, and 1800 data points for both the control and TTX data. The sampled data from the control and TTX dataset were compared within themselves as well as between each other (i.e., control to control, TTX to TTX, and control to TTX); 100 scaling factors were obtained in each case. A cumulative frequency distribution was plotted for the scaling factors obtained in the case of control versus control, TTX versus TTX, and control versus TTX comparison for the sampling levels 200 (A), 400 (B), 600 (C), 800 (D), 1000 (E), 1200 (F), 1400 (G), 1600 (H), 1800 (I). J, The scaling factors obtained for each sampling level was compared between the control versus control, TTX versus TTX, and control versus TTX dataset with the help of a KS test, and the p value was plotted against the sampling level. Download Figure 2-3, EPS file.