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Research ArticleResearch Article: Confirmation, Integrative Systems

Arousal State-Dependent Alterations in VTA-GABAergic Neuronal Activity

Ada Eban-Rothschild, Jeremy C. Borniger, Gideon Rothschild, William J. Giardino, Joshua G. Morrow and Luis de Lecea
eNeuro 13 February 2020, 7 (2) ENEURO.0356-19.2020; DOI: https://doi.org/10.1523/ENEURO.0356-19.2020
Ada Eban-Rothschild
1Department of Psychology, University of Michigan, Ann Arbor, MI 48109
2Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305
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Jeremy C. Borniger
2Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305
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Gideon Rothschild
1Department of Psychology, University of Michigan, Ann Arbor, MI 48109
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William J. Giardino
2Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305
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Joshua G. Morrow
1Department of Psychology, University of Michigan, Ann Arbor, MI 48109
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Luis de Lecea
2Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305
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Abstract

Decades of research have implicated the ventral tegmental area (VTA) in motivation, learning and reward processing. We and others recently demonstrated that it also serves as an important node in sleep/wake regulation. Specifically, VTA-dopaminergic neuron activation is sufficient to drive wakefulness and necessary for the maintenance of wakefulness. However, the role of VTA-GABAergic neurons in arousal regulation is not fully understood. It is still unclear whether VTA-GABAergic neurons predictably alter their activity across arousal states, what is the nature of interactions between VTA-GABAergic activity and cortical oscillations, and how activity in VTA-GABAergic neurons relates to VTA-dopaminergic neurons in the context of sleep/wake regulation. To address these, we simultaneously recorded population activity from VTA subpopulations and electroencephalography/electromyography (EEG/EMG) signals during spontaneous sleep/wake states and in the presence of salient stimuli in freely-behaving mice. We found that VTA-GABAergic neurons exhibit robust arousal-state-dependent alterations in population activity, with high activity and transients during wakefulness and REM sleep. During wakefulness, population activity of VTA-GABAergic neurons, but not VTA-dopaminergic neurons, was positively correlated with EEG γ power and negatively correlated with θ power. During NREM sleep, population activity in both VTA-GABAergic and VTA-dopaminergic neurons negatively correlated with δ, θ, and σ power bands. Salient stimuli, with both positive and negative valence, activated VTA-GABAergic neurons. Together, our data indicate that VTA-GABAergic neurons, like their dopaminergic counterparts, drastically alter their activity across sleep-wake states. Changes in their activity predicts cortical oscillatory patterns reflected in the EEG, which are distinct from EEG spectra associated with dopaminergic neural activity.

  • arousal
  • dopamine
  • fiber photometry
  • GABA
  • VTA

Significance Statement

Little is known about how ventral tegmental area (VTA) neural ensembles couple arousal to motivated behaviors. Using cell type-specific genetic tools, we investigated the population activity of GABAergic and dopaminergic neurons within the VTA across sleep/wake states and in the presence of salient stimuli. We demonstrate that coordinated neural activity within VTA-GABAergic neurons peaks during wakefulness and REM sleep. Furthermore, neuronal activity in VTA-GABAergic neurons is correlated with high frequency, low amplitude cortical oscillations during waking, but negatively correlated with high amplitude slower frequency oscillations during NREM sleep. Our results demonstrate that VTA-GABAergic neuronal activity is tightly linked to cortical arousal and highlight this population as a potential important node in sleep/wake regulation.

Introduction

Motivated behaviors are fundamentally linked to arousal, however, little is known about the neural mechanisms that couple motivational processes with sleep-wake regulation (Eban-Rothschild et al., 2018). The ventral tegmental area (VTA), which is comprised of 55–65% dopaminergic, 35–40% GABA, and ∼2% glutamatergic neurons (Nair-Roberts et al., 2008; Morales and Margolis, 2017), is a key brain structure in the regulation of motivation, reinforcement learning and reward processing (Berridge, 2007; Salamone and Correa, 2012; Berke, 2018). VTA-GABAergic neurons have been suggested to tightly control activity in intermingled VTA-dopaminergic neurons in the context of motivation and reward (Cohen et al., 2012; van Zessen et al., 2012), yet their regulatory role in the context of sleep and wakefulness is not fully understood.

Neuronal activity in the VTA exhibits arousal-state-dependent alterations, with increased activity during wakefulness and REM sleep compared with NREM sleep (Miller et al., 1983; Fifel et al., 2018). Although initial studies suggested that VTA-dopaminergic neurons do not change their mean firing rate across sleep/wake states (Miller et al., 1983; Steinfels et al., 1983), more recent examinations revealed marked arousal state-dependent alterations in both firing rate and firing pattern (Dahan et al., 2007; Gomperts et al., 2015). Moreover, using cell type-specific fiber photometry recordings, we demonstrated that at the population level, VTA-dopaminergic neurons show strong arousal-state-dependent alterations in activity (Eban-Rothschild et al., 2016). Early single-unit electrophysiology recordings in rats suggested that activity in VTA-GABAergic neurons varies significantly across arousal state, with high firing rates during active waking and REM sleep (Lee et al., 2001). An additional study demonstrated activation, via cFos immunolabeling, of VTA-GABAergic neurons following recovery from REM sleep deprivation (Maloney et al., 2002), suggesting that these neurons influence or monitor discrete arousal states. Nonetheless, there is a great challenge to accurately differentiate between different cell types in freely moving wild-type animals and conventional electrophysiological characteristics used to differentiate between VTA cell types has been debated (Margolis et al., 2006; Lammel et al., 2008; Ungless and Grace, 2012). Moreover, it is still unclear whether activity in VTA-GABAergic neurons precedes or lags behind arousal state transitions, how population activity in the VTA is related to different components of the cortical electroencephalography (EEG) and how VTA-GABAergic population dynamics relate to that of co-mingled dopaminergic neurons. A few recent studies have shed some light onto these questions, demonstrating that VTA-GABAergic neurons bidirectionally regulate wakefulness in part via their projections to the lateral hypothalamus (LH; Takata et al., 2018; Chowdhury et al., 2019; Yu et al., 2019b). However, there are some critical discrepancies between these studies, as one suggests that VTA-GABAergic neurons are predominantly wake and REM sleep active (Yu et al., 2019b), while the other provides evidence that these neurons fire mostly during NREM sleep (Chowdhury et al., 2019). Therefore, the exact phenotype of VTA-GABAergic neurons across arousal states remains unclear.

To address these questions, we used Cre-dependent viral vectors to express the calcium indicator GCaMP6 exclusively in either VTA-GABAergic or VTA-dopaminergic neurons. We then used fiber photometry in tandem with EEG/electromyography (EMG) recordings to monitor spontaneous arousal-state-dependent changes in population activity. We also recorded calcium and EEG measures during a battery of behavioral challenges (social and non-social).

Materials and Methods

Animals

We used adult (more than eight weeks old) male vesicular GABA transporter (Vgat)-IRES-Cre (The Jackson Laboratory stock #028862) knock-in mice backcrossed on the C57BL/6J wild-type background (for >10 generations) and tyrosine hydroxylase (Th)-IRES-Cre mice obtained from the European Mouse Mutant Archive and backcrossed on the C57BL/6J wild-type background (for >15 generations). During experiments, mice were individually housed in custom Plexiglas recording chambers at a constant temperature (23 ± 1°C), humidity (40–60%), and 12/12 h light/dark cycle. Food (Laboratory Rodent Diet #5001) and water were available ad libitum. All experiments were performed in accordance with the guidelines described in the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by Stanford University Administrative Panel on Laboratory Animal Care.

Virus preparation

Cre-dependent recombinant adeno-associated virus (AAV) vectors (pAAV-Ef1α-DIO-GCaMP6s, pAAV-Ef1α-DIO-GFP, and pAAV-Ef1α-DIO-GCaMP6f) were serotyped with AAV-DJ coat proteins and packaged by the Gene Vector and Virus Core at Stanford University. The final viral concentration was 1–1.5 × 1013 genome copies (gc) ml−1. Aliquots of virus were stored at −80°C until stereotaxic injection.

Surgery

At the start of surgical procedures, mice were anesthetized with ketamine and xylazine (100 and 20 mg kg−1, respectively, i.p.) and placed into a stereotaxic frame (David Kopf Instruments). To selectively target subpopulations of VTA neurons, virus was infused into the VTA (AP −3.3 mm; ML = 0.2 mm; DV = −4.6 mm) of either Vgat-Cre or Th-Cre mice, using a 33-guage internal cannula (Plastics One, Inc.) attached to a 10-μl Hamilton syringe, at a rate of 0.1 μl min−1 (400-nl total virus). After infusion, the internal cannula was kept in place for at least 8 min and then slowly withdrawn. Following virus injections, a 400 μm in diameter monofiberoptic cannula (Doric lenses, Inc.) was placed above the VTA (AP = −3.3, ML = 0.3, DV −4.3 mm) for fiber photometry recordings. Mice were also fitted with two gold-plated miniature screw electrodes (AP = 1.5 mm, ML = −1.5 mm, AP = −3.5 mm, ML = −2.8 mm) and two EMG wire electrodes (316SS/44T, Medwire), previously soldered to a four-pin connector (custom). These electrodes were inserted between the trapezius muscles for EMG measurements. The implant was secured to the skull with C&B Metabond (Parkell) and dental cement. The skin was closed with surgical sutures, and mice were maintained on a heating pad until fully mobile. Mice were allowed to recover from surgery for three weeks, and then acclimated to a flexible EEG/EMG connection cable and optical patch cord for ∼10 d within individual recording chambers. Mice were continuously connected to the patch cord and EEG/EMG connection cable throughout the experiments.

Fiber photometry recordings and data analysis

Fiber photometry recordings were conducted as previously described (Lerner et al., 2015; Eban-Rothschild et al., 2016). We sinusoidally modulated blue light (30–50 μW) from a 470 nm excitation LED (M470F3, Thorlabs) at 211 Hz, using a custom MATLAB script (MathWorks) and a multifunction data acquisition device (NI USB-6259, National Instruments). The blue light was passed through a GFP excitation filter (MD498, Thorlabs), reflected off a dichroic mirror (MD498, Thorlabs), and coupled using a fiber collimation package (F240FC-A, Thorlabs) into a low-fluorescence patch cord (400 μm, 0.48 NA; Doric Lenses) connected to the implanted optic fiber (400 μm) by a zirconia sleeve. GCaMP6 signal was collected through the excitation patch cord, passed through a GFP emission filter (MF525-39, Thorlabs), and focused onto a photodetector (Model 2151, Newport) using a lens (LA1540-A, Thorlabs). The signal was then sent to a lock-in amplifier (30-ms time constant, Model SR830, Stanford Research Systems) that was synchronized to 211 Hz. Signals from the amplifiers were collected at 1 kHz using a custom MATLAB script and a multifunction data acquisition device (National Instruments).

The photometry signal was down-sampled using interpolation to match the EEG sampling rate of 256 Hz. For each experiment, the photometry signal F was converted to ΔF/F by Embedded Image . To address a short decay at the beginning of some recording sessions, the ΔF/F signal from each session was fitted with a decreasing exponential of the form a x ebx, where a > 0 and b < 0. We then subtracted the exponential fit from the original ΔF/F signal. To detect calcium transients, we generated two filtered ΔF/F signals, one low-pass filtered at 0–0.4 Hz, and the other low-pass filtered at 0–40 Hz. We calculated a trace of the derivative of their squared difference and identified candidate transient times by thresholding this signal at mean +1.5 SD. We further required that the ΔF/F of candidate transient times be higher than the mean +1.5 SD of the trace. The exact peak time of each transient was identified by taking the maximal value of the thresholded signal within each transient. For each session and for each arousal state, we calculated the mean transient rate (calculated as the number of transients per second) and mean transient amplitude (calculated as the mean ΔF/F at transient peak times).

For sleep-wake analyses of Vgat-cre mice, we recorded data during seven to eight sessions per mouse (n = 5), each 30–40 min long, during the light phase. For sleep-wake analyses of Th-cre mice, we recorded data during eight sessions per mouse (n = 4), each 10- to 45-min-long during the light phase. We used only sessions during which mice showed all three arousal states (wake, NREM sleep, and REM sleep). For each session we calculated the mean ΔF/F during all times of wake, NREM sleep, and REM sleep. For state transition analyses, we identified all points of state transition and aligned ΔF/F around these times. Mean ΔF/F and transient rate of the Th-Cre mice analyzed in this paper were previously reported in Eban-Rothschild et al. (2016).

EEG/EMG recording and analysis

EEG/EMG signals were amplified (Grass Technologies) and digitized at 256 Hz using Vital Recorder software (Kissei Comtec America). Using analysis software (SleepSign for Animal, Kissei Comtec America) we filtered the signal (EEG: 0.3–80 Hz, EMG: 25–50 Hz) and spectrally analyzed it by fast Fourier transformation to determine a single arousal state (wake, NREM sleep, or REM sleep) for each 4-s epoch of data. Wakefulness was defined as desynchronized low-amplitude EEG and heightened tonic EMG activity with phasic bursts. NREM sleep was defined as synchronized, high-amplitude, low-frequency EEG and substantially reduced EMG activity compared with wakefulness, with no phasic bursts. REM sleep was defined as having a pronounced θ rhythm (5–9 Hz) with no EMG activity. The first epoch during which a pronounced θ rhythm was detected, together with reduced EEG amplitude and EMG integral, was defined as the epoch of NREM to REM transition. For all further EEG data analyses, raw (not filtered) EEG/EMG data were exported from the sleep analysis software (SleepSign for Animal) and imported into MATLAB using a custom script. To estimate EEG power in a specific band, we filtered the EEG in that band and took the absolute value of the Hilbert transform of the filtered trace.

Salient stimuli presentation

We gently introduced salient stimuli to the home cage of test mice that were individually housed and connected to fiber optic and EEG/EMG recording cables. In each experimental session, each test mouse was exposed to one stimulus (n = 4–5 per stimulus). Different stimuli were presented several days apart. Salient stimuli were: (1) 8- to 12-week-old female C57BL/6J mice (The Jackson Laboratory), (2) ex-breeder CD1 male mice (more than four months old), (3) four-week-old male C57BL/6J mice, (4) high-fat chow (D12451, Research Diets, Inc.), and (5) rat bedding. We provided test mice with a small piece (0.3 g) of high-fat chow during the 2 d preceding the high-fat chow experiment. We recorded fiber photometry and EEG/EMG signal prior to and following the introduction of the salient stimuli.

Immunohistochemistry

Following completion of experiments, mice were deeply anesthetized with ketamine and xylazine (100 and 20 mg kg−1, respectively, i.p.) and transcardially perfused with 1× PBS followed by 4% paraformaldehyde (PFA) in PBS. Brains were removed and postfixed overnight in 4% PFA. Brains were then cryoprotected in 30% sucrose for minimum 48 h at 4°C, and then sectioned (30 μm) on a cryostat (Leica Microsystems), collected in PBS containing 0.1% NaN3, and stored at 4°C.

Sections underwent immunohistochemical staining as described below. Primary antibodies were used at 1:1000 dilution: chicken anti-TH (#TYH, Aves Labs, Inc.) and rabbit anti-GABA (#20094, IMMUNOSTAR). Immunofluorescent secondary antibodies were used at 1:500 dilution: donkey anti-chicken Alexa Fluor 594 (#703-585-155, Jackson ImmunoResearch) for GCaMP6 and TH co-localization, and donkey anti-rabbit Alexa Fluor 594 (#711-585-152, Jackson ImmunoResearch) for GCaMP6 and GABA co-localization.

For immunofluorescence staining, brain sections were washed in PBS for 5 min and then incubated in a blocking solution composed of PBS with 0.3% Triton X-100 (PBST) containing 4% bovine serum albumin for 1 h. Sections were then incubated for ∼16 h in block solution containing primary antibodies. After three 5-min washes in PBS, sections were incubated in blocking solution for 1 h and then incubated for 2 h in blocking solution containing secondary antibodies. Sections were washed three times for 5 min each in PBS, mounted onto gelatin-coated microscope slides (FD Neurotechnologies, #PO101) and coverslipped with Fluoroshield containing DAPI Mounting Media (Sigma, #F6057).

Microscopy and image analysis

Images were collected on a Zeiss LSM 710 confocal fluorescent microscope using reflected light. Digital images were minimally processed using ImageJ (NIH) to enhance brightness and contrast for optimal representation.

Statistical analyses

Sample sizes were chosen based on previous publications for the study of the sleep-wake circuitry. We used two-way repeated-measures (RM) ANOVAs for analyses of calcium signal and transient rate (Figs. 1E,G, 5F; factor 1: “mice,” factor 2: “arousal state”). We used two-tailed paired t tests for data presented in Figure 1F,H. To correct for the multiple comparisons in these analyses we used a Bonferroni correction, i.e., we multiplied the obtained p values by the number of comparisons (×3). We used correlation coefficient to determine the correlation between fluorescence and power in specific frequency bands (Figs. 3–5). To correct for the multiple comparisons in the analyses of correlation between fluorescence and power in specific frequency bands we used a Bonferroni correction, and multiplied the obtained p values by the number of comparisons [wake, ×2 (θ and γ comparisons); NREM, ×3 (δ, θ, and σ comparisons); REM, ×2 (θ and γ comparisons)]. We analyzed data using Prism 6.0 (GraphPad Software) and MATLAB R2016b software. Data are presented as mean ± SEM unless otherwise indicated. For the preparation of the figures, we used MATLAB R2016b and Prism 6.0 and then exported the data into Adobe Illustrator CS6 (Adobe Systems).

Figure 1.
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Figure 1.

VTA-GABAergic neurons show arousal-state-dependent alterations in population activity. A, Confocal microscopic images of AAV-DJ-EF1α-DIO-GCaMP6s expression in the VTA co-immunostained with anti-GABA (top) or anti-TH (bottom) antibodies, demonstrating selective calcium indicator expression in VTA-GABAergic neurons, but not VTA-dopaminergic neurons. B, Schematic for simultaneous recordings of VTA-GABA-GCaMP6s and EEG/EMG signals in Vgat-Cre mice. C, Representative image of GCaMP6s-expressing neurons in the VTA and the fiber optic tract for recording. D, Representative fluorescence trace color-coded by arousal state (wake, NREM sleep, and REM sleep). Identified transients are denoted by open circles. E, Mean ± SEM fluorescence during wake, NREM sleep, and REM sleep (two-way RM ANOVA between mice and arousal state; mice, F(7,31) = 0.006, p > 0.99, arousal state, F(2,62) = 8.075, p = 0.0008, followed by Tukey’s post hoc tests, wake vs NREM p > 0.99, wake vs REM p = 0.0027, NREM vs REM p = 0.0027). F, Scatter plots comparing mean %ΔF/F values for pairs of arousal states (paired samples t tests, wake vs NREM, t(38) = −9.8881, p = 4.6 × 10−12; REM vs NREM, t(38) = −14.8166, p = 2.2 × 10−17; wake vs REM, t(38) = −6.387, p = 1.68 × 10−7). Each dot represents a single trial. The different colors represent different mice. G, Transient rate (Hz) for wake, NREM sleep, and REM sleep (two-way RM ANOVA between mice and arousal state; mice, F(7,31) = 0.7776, p = 0.61, arousal state, F(2,62) = 117.8, p < 1 × 10−15, followed by Tukey’s post hoc tests, wake vs NREM = 4.39 × 10−10, wake vs REM p = 3.99 × 10−10, NREM vs REM p = 1.89 × 10−11). H, Scatter plots comparing mean transient rate values among each pair of arousal state (paired samples t tests, wake vs NREM, t(38) = −10.2078, p = 1.9 × 10−12; REM vs NREM, t(38) = −20.1677, p = 6.7 × 10−22; wake vs REM, t(38) = −6.3469, p = 1.9 × 10−7). Each dot represents a single trial. The different colors represent different mice. *, 0.001 < p ≤ 0.01; ****, p ≤ 0.0001.

Results

VTA-GABAergic neuronal activity is modulated across arousal state

To assess population activity of VTA-GABAergic neurons across spontaneous sleep-wake states we used calcium-based fiber photometry recordings (Fig. 1A–C). We injected a Cre-dependent AAV encoding the fluorescent calcium indicator GCaMP6s (pAAV-DJ-EF1α-DIO-GCamP6s) into the VTA of Vgat-IRES-Cre knock-in mice and implanted a fiber optic probe above the VTA for subsequent delivery of excitation light and collection of fluorescent emission and with EEG-EMG electrodes for simultaneous sleep-wake recordings. Fluorescent immunostaining for GABA and TH in GCaMP6-expressing slices demonstrated specific co-expression of GCaMP6 in GABAergic, but not dopaminergic VTA neurons (Fig. 1A). We found robust alterations in both fluorescence signal (%ΔF/F) and calcium transient rate (Hz) in VTA-GABAergic neurons over spontaneous sleep/wake states (Fig. 1D–H). During NREM sleep, VTA-GABAergic neurons showed the lowest fluorescence and calcium transient rates compared with both wakefulness and REM sleep (Fig. 1D–H). In contrast, VTA-GABAergic neurons showed the most frequent calcium transients during REM sleep compared with both wakefulness and NREM sleep (Fig. 1G,H). These arousal-state-dependent alterations in activity were not found in control experiments (data not shown).

We proceeded to determine whether activity in VTA-GABAergic neurons is altered around arousal-state transitions. VTA-GABAergic neurons showed a strong decrease in population activity at wake to NREM transitions (Fig. 2A,B), while EEG δ power was strongly increased (Fig. 2C). These alterations were not found in control experiments (data not shown). NREM sleep to wakefulness transitions were accompanied by a large increase in population activity and a decrease in EEG δ power (Fig. 2A–C), even for short (<12 s) wake episodes (data not shown). During NREM to REM sleep transitions, the ΔF/F signal showed a gradual ramping 10–20 s prior to the vigilance state transition, subsequently peaking ∼20 s following the transition (Fig. 2A,B). Following REM sleep to wake transitions, a reduction in VTA-GABAergic calcium fluorescence was observed (Fig. 2A,B). Together, these findings demonstrate that VTA-GABAergic neurons exhibit strong alterations in population activity across arousal states.

Figure 2.
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Figure 2.

VTA-GABAergic population dynamics around arousal state transitions. A, Heatmaps of fluorescence intensity aligned to arousal state transitions (wake to NREM, n = 459; NREM to wake, n = 329; NREM to REM, n = 135; REM to wake, n = 135). Trials are sorted by the duration of the second state (shortest, top; longest, bottom). B, Average responses from all the transitions expressed as mean (blue trace) ± SEM (gray shading). C, EEG δ (1–4.5 Hz) power from all the transitions.

VTA-GABAergic neuronal activity correlates with specific cortical oscillatory patterns

To investigate whether VTA-GABAergic neural activity is correlated with specific components of the EEG during different vigilance states, we examined correlations between population activity and power within specific EEG bands during 1-s epochs of wakefulness, NREM sleep, and REM sleep. During wakefulness, we found a negative correlation between VTA-GABAergic population activity and EEG θ (5–9 Hz) power, and a positive correlation of population activity and EEG γ (30–80 Hz) power (Fig. 3A,B; θ: R(19,730) = −0.18, p < 0.0001; γ: R(19,730) = 0.33, p < 0.0001). The positive γ correlation was evident across all test mice, and was absent in control experiments (data not shown). In contrast, population activity of VTA-dopaminergic neurons was not correlated with the EEG γ band, and only weakly correlated with θ (Fig. 4A,B; θ: R(12,884) = −0.05, p < 0.0001; γ: R(12,884) = 0.01, p = 0.1). These data suggest that during wakefulness, activity of VTA-GABAergic, but not dopaminergic, neurons is related to fast cortical oscillations.

Figure 3.
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Figure 3.

VTA-GABAergic population activity is positively correlated with fast-frequency EEG oscillations during wakefulness and negatively correlated with low-frequency oscillations during NREM sleep. Correlations between EEG spectral components and population activity during spontaneous wakefulness (A, B), NREM sleep (C–E), REM sleep (F, G), and across the three states (H). Each dot represents a 1-s epoch. Dashed line is the best linear fit. R is correlation coefficient. Right panels in each arousal state section represent a: representative raw EEG trace, fluorescence trace, filtered EEG, and power in a specific EEG band for a 2- to 10-s wake epoch.

Figure 4.
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Figure 4.

Correlations between EEG spectral components and VTA-dopaminergic neural activity during wakefulness, NREM sleep, and REM sleep. VTA-dopaminergic population activity plotted against EEG θ (A) and γ (B), power during wakefulness. VTA-dopaminergic population activity plotted against EEG δ (C), θ (D), and σ (E) power during NREM sleep. VTA-dopaminergic population activity plotted against EEG θ (F) and γ (G) power during REM sleep. Each dot represents a 1-s epoch. Dashed line is the best linear fit. R is correlation coefficient.

During NREM sleep, we observed a negative correlation between VTA-GABAergic population activity and EEG δ (1–4.5 Hz), θ, and σ (10–14 Hz) power bands [Fig. 3C–E; R(43,810) = −0.04 (δ), −0.12 (θ), and −0.15 (σ), p < 0.0001]. Moreover, power in these frequency bands was significantly lower during high ΔF/F times as compared with low ΔF/F times (data not shown). Similar to VTA-GABAergic neurons, VTA-dopaminergic neurons showed a negative correlation between population activity and EEG δ, θ, and σ power bands during NREM sleep [Fig. 4C–E; R(33,467) = −0.06 (δ), −0.07 (θ), and −0.07 (σ), p < 0.0001]. Together, our findings suggest that within NREM sleep, high activity periods in both VTA-GABAergic and VTA-dopaminergic neurons are associated with a lightening of NREM sleep.

Within REM sleep, we found a significant, yet weak, negative correlation between VTA-GABAergic population activity and both EEG θ and γ power bands [Fig. 3F,G; R(43,810) = −0.06 (γ) and −0.05 (θ), p < 0.0001]. In contrast, VTA-dopaminergic neurons showed an opposite, yet weak, trend for EEG θ power and no correlation for EEG γ power (Fig. 4D,E).

We also determined the correlation between VTA-GABAergic neural activity and specific components of the EEG across arousal states. We found that VTA-GABAergic population activity is negatively correlated to δ, θ, and σ power bands [R(71,926) = −0.3 (δ), −0.31 (θ) and −0.34 (σ), p < 0.0001] and positively correlated with EEG γ power (R(71,926) = 0.13, p < 0.0001). Taken together, our findings demonstrate that activity in VTA-GABAergic neurons is strongly associated with both behavioral and electrocortical arousal.

VTA-GABAergic neurons exhibit biased responses to social stimuli

To further explore the relationship between activity in VTA-GABAergic neurons and arousal, we presented mice with various salient stimuli, of both positive and negative valences, while simultaneously recording population activity from VTA-GABAergic neurons and EEG/EMG signals. Following the placement of salient stimuli inside the home cage of test mice, VTA-GABAergic neurons showed a fast increase in population activity (Fig. 5A–E). Social stimuli (female mouse, juvenile mouse and an aggressive mouse) induced an increase in activity that remained elevated for >120 s, irrespective of valence (Fig. 5A–C). Non-social salient stimuli, associated with both positive (high-fat chow) and negative (rat bedding) valences, induced much weaker activation of VTA-GABAergic neurons (Fig. 5D–F). As during spontaneous wakefulness, population activity of VTA-GABAergic neurons showed a positive correlation with EEG γ power during the presentation of most salient stimuli (Fig. 5G–K). Surprisingly, no correlation between population activity of VTA-GABAergic neurons and EEG γ power was found during trials in which male mice were presented (both young and older/aggressive males; Fig. 5G–K). Together, these findings further support the premise that VTA-GABAergic neurons activity is tightly linked to behavioral and electrocortical arousal.

Figure 5.
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Figure 5.

VTA-GABAergic neurons are strongly activated by social salient stimuli. A–E, Population activity of VTA-GABAergic neurons prior and following the placement of (A) a female mouse, (B) an aggressive (CD1) male mouse, (C) a juvenile male mouse, (D) high-fat rodent chow, and (E) rat bedding, inside the home cage of test mice. Presented are 60 s prior to and 120 s following stimulus presentation. F, Summary data showing the relative change in %ΔF/F for each stimulus prior to and after presentations (two-way ANOVA: main effect of time F(1,19) = 95.92, p < 0.0001; main effect of stimuli F(4,19) = 5.324, p = 0.0048; and the interaction F(4,19) = 5.759, p = 0.0033). Sidak’s multiple comparisons post hoc tests reveal stimulus-specific responses in the %ΔF/F signal (no differences were observed during baseline prior to stimulus presentation); after stimulus presentation, female mouse versus aggressive mouse t = 0.8874, p > 0.05, female mouse versus juvenile male mouse t = 0.8488, p > 0.05, female mouse versus high-fat diet t = 3.901, p = 0.0038, female mouse versus rat (predator) bedding t = 4.391, p = 0.0009, aggressive mouse versus juvenile mouse t = 1.736, p > 0.05, aggressive mouse versus high-fat diet t = 3.014, p = 0.0448, aggressive mouse versus rat bedding t = 3.555, p = 0.0103, juvenile mouse versus high-fat diet t = 4.75, p = 0.0003, juvenile mouse versus rat bedding t = 5.192, p < 0.0001, high-fat diet versus rat bedding t = 0.7133, p > 0.05. Before and after stimulus comparisons further allow for specific effects of each stimulus to be assessed (Sidak’s): female mouse t = 5.983, p < 0.0001, aggressive mouse t = 5.459, p = 0.0001, juvenile mouse t = 7.249, p < 0.0001, high-fat diet t = 2.474, p > 0.05, rat bedding t = 1.14, p > 0.05. Note that before and after stimulus differences are only significant for social stimuli (female and male mice), but not for nonsocial stimuli (high-fat chow and rat bedding). G–K, Correlations between EEG γ power and VTA-GABAergic neural activity following the presentation of salient stimuli. Each dot represents a 1-s epoch. Dashed line is the best linear fit. R is correlation coefficient. NS, p > 0.05; *, 0.01 < p ≤ 0.05; **, 0.001 < p ≤ 0.01; ***, 0.0001 < p ≤ 0.001; ****, p ≤ 0.0001.

Discussion

Here, we investigated the relationships between electrocortical and behavioral arousal and population activity of subsets of neurons in the VTA. We observed that activity in VTA-GABAergic neurons varies significantly across arousal states. VTA-GABAergic neurons are strongly activated during wakefulness and REM sleep and show low population activity during NREM sleep. Moreover, during NREM sleep, population activity of VTA-GABAergic neurons negatively correlates with NREM sleep intensity, i.e., δ, θ, and σ power bands. In contrast, during wakefulness population activity of VTA-GABAergic neurons is positively correlated with EEG γ power. We also found that VTA-GABAergic neurons are strongly activated by different salient stimuli, specifically social stimuli of both positive and negative valence. Activity of VTA-dopaminergic neurons was found to be similarly negatively correlated with NREM sleep intensity but, notably, we did not observe a significant correlation between population activity and γ power during wakefulness. Together, our findings demonstrate that the VTA, which is mainly studied in regard to reward, motivation and learning, also shows strong modulation by arousal state.

Both VTA dopaminergic and GABAergic neurons are strongly activated by rewards and reward-predictive cues, yet VTA-GABAergic neurons show sustained excitation following a reward-predictive cue while activity in VTA-dopaminergic neurons is suppressed in the delay period between cue and reward delivery (Cohen et al., 2012). It has been suggested that sustained activation of VTA-GABAergic neurons during the delay period accounts for the suppression of VTA-dopaminergic neural activity and possibly the computation of reward prediction error (Schultz, 1998; Cohen et al., 2012). VTA-GABAergic neurons inhibit VTA-dopaminergic neurons both in vitro (Dobi et al., 2010; van Zessen et al., 2012) and in anesthetized rats (Tan et al., 2012). It has been proposed that VTA-GABAergic neurons do not exert a tonic inhibition on VTA-dopaminergic neurons, but rather sculpt their activity pattern in favor of fast oscillations (Brown and McKenna, 2015). In the context of sleep/wake regulation, we have previously demonstrated that VTA-dopaminergic neurons increase their activity prior to NREM sleep-to-wake transitions (Eban-Rothschild et al., 2016), while here, we report that VTA-GABAergic neurons increase their activity in conjunction with these transitions (Fig. 2). It is thus unlikely that VTA-GABAergic neurons drive NREM sleep-to-wake transitions. Rather, VTA-GABAergic neurons may suppress activity in VTA-dopaminergic neurons following a sleep-to-wake transition, to tightly control arousal. Indeed, lesioning or chemogenetic inhibition of VTA-GABAergic neurons produces persistent wakefulness (Takata et al., 2018; Yu et al., 2019b). VTA-dopaminergic neural activation potently promotes arousal and maintains long-term wakefulness (Eban-Rothschild et al., 2016; Taylor et al., 2016; Oishi et al., 2017), and the increased activity in VTA-dopaminergic neurons suppresses the motivation to prepare for sleep and engage in sleep-preparatory behaviors (Eban-Rothschild et al., 2016). It is reasonable to assume that activity in VTA-dopaminergic neurons is tightly regulated to suppress wakefulness when sleep is in need and prevent a state of hyperarousal (Yu et al., 2019a), as may be the case in mania or drug-induced arousal. Cocaine, for example, reduces activity in VTA-GABAergic neurons, which results in disinhibition of VTA-dopaminergic neurons and the elevation of extracellular dopamine concentrations (Bocklisch et al., 2013). Similarly, optogenetic inhibition of VTA-GABAergic neurons results in disinhibition of VTA-dopaminergic neurons and an increase in VTA-dopaminergic neuronal activation (Bocklisch et al., 2013). Together, our findings provide a mechanistic framework for the participation of dopaminergic and GABAergic VTA neurons in the regulation of arousal. A better understanding of VTA microcircuitry in the context of arousal regulation holds great promise to improve the capacity to treat sleep dysregulation in pathologic circumstances, such as drug addiction.

It is important to mention that in the current study we did not distinguish between subpopulations of GABAergic neurons. For example, some VTA-GABAergic neurons express corticotropin-releasing factor (CRF)-binding protein (Wang and Morales, 2008), dopamine receptor D2, or μ-opioid receptors (Margolis et al., 2012). In addition, some VTA-GABAergic interneurons form local connections within the VTA while others send long-range projections (Morales and Margolis, 2017). It would be of interest for future studies to determine whether these disparate populations differentially influence arousal. Recently, Yu and colleagues made progress in this regard. They demonstrated that VTA-GABAergic neurons project to the LH, where they inhibit wakefulness and drive NREM sleep (Yu et al., 2019b). When VTA-GABAergic neural activity was monitored using fiber photometry, they observed high calcium fluorescence during wakefulness and REM sleep, similar to our findings. In contrast, Chowdhury and colleagues recently observed that GAD67+ GABAergic neurons in the VTA are mostly active during NREM sleep (Chowdhury et al., 2019). The discrepancy between the different studies may be due to the use of a different Cre-line to target GABAergic neurons in the VTA (Vgat-cre vs GAD67-cre), or to the targeting of different VTA sub-regions. While Yu et al. (2019b) and our study targeted relatively rostral regions of the VTA (3.3 mm posterior to bregma), Chowdhury et al. (2019) may have targeted caudal regions of the VTA (3.0–3.7 mm posterior to bregma). Indeed, GABAergic neurons in the tail of the VTA, or RMTg, have been demonstrated to promote NREM sleep via inhibition of VTA dopaminergic neurons (Yang et al., 2018). The different studies (Yu et al. 2019b; Chowdhury et al., 2019) also used different viral constructs and/or promoters that may have influenced the viral tropism and expression levels of GCaMP in the VTA. It is also important to note that while Chowdhury and colleagues reported higher population activity in VTA GAD67+ neurons during NREM sleep compared with wakefulness and REM sleep, examination of their data suggests an increase in population activity immediately following sleep-to-wake transitions, and higher transient rates during wakefulness and REM sleep compared with NREM sleep (Chowdhury et al., 2019). Broadly, GABAergic neurons are highly heterogeneous across the brain (Jones, 2017), and the use of bulk population activity recordings may mask ensemble differences in activity patterns. Nonetheless, using microendoscope calcium imaging of VTA-GABAergic neurons Yu and colleagues reported a similar Wake on/NREM off activity pattern, although their sample size was relatively low (Yu et al., 2019b). Further research is needed to tease apart how specific subsets of VTA-GABAergic neurons contribute to arousal state regulation.

How are VTA-GABAergic neurons linked to other sleep/wake regulatory centers? VTA-GABAergic neurons receive excitatory, inhibitory and modulatory inputs from diverse cortical and subcortical areas (Beier et al., 2015; Faget et al., 2016). Putative VTA-GABAergic neurons are strongly activated by different arousal-related neuromodulators including hypocretin, CRF, substance P, and histamine (Korotkova et al., 2002, 2006). VTA-GABAergic neurons send widespread projections to various forebrain and brainstem areas including the basal forebrain, LH, periaqueductal gray and dorsal raphe nuclei, as well as sparser projections to the nucleus accumbens and prefrontal cortex (Carr and Sesack, 2000b; Kirouac et al., 2004; Brown et al., 2012; van Zessen et al., 2012; Taylor et al., 2014). Together, these observations demonstrate that VTA-GABAergic neurons are well positioned to regulate local and global neuronal activity for the support of electrocortical and behavioral arousal.

We found that VTA-GABAergic neural activity correlated with specific components of the EEG power spectrum. Across arousal states, δ, θ, and σ power bands were negatively correlated with VTA-GABAergic activity (Fig. 3). Slow oscillations in the EEG are related to the depth of sleep, correlate with the duration of prior waking, and are a signature of widespread synchronization of cortical and thalamo-cortical networks (Contreras et al., 1996; Cirelli, 2009; Borbély et al., 2016). Sleep spindles, periodic oscillatory patterns in the NREM EEG that overlap with the σ power band (10–14 Hz), are thought to stabilize sleep and promote memory consolidation (Mednick et al., 2013; Rasch and Born, 2013). Spindles are primarily generated by the thalamic reticular nucleus (RTN), where GABAergic neurons function as a pacemaker, and glutamatergic thalamo-cortical projections mediate the propagation of spindles to the cortex (von Krosigk et al., 1993; Contreras et al., 1996; De Gennaro and Ferrara, 2003). A similar pattern was observed for VTA-dopaminergic neurons, where high activity within this population was negatively correlated with EEG δ, θ, and σ bands during NREM sleep. Negative correlations between VTA neural activity and cortical NREM sleep characteristics suggest that the concerted activity of these neurons may fundamentally oppose slow oscillations. This may underlie chronic sleep disturbances in people who abuse drugs targeting the dopaminergic system (e.g., cocaine, methamphetamine).

We also found that activity in VTA-GABAergic, but not dopaminergic neurons, is positively correlated with EEG γ power during both spontaneous and motivated wakefulness (Figs. 3-5). A previous study demonstrated that activity in the VTA and prefrontal cortex (PFC) is coupled via a 4-Hz oscillation that synchronizes local γ oscillations and neuronal firing (Fujisawa and Buzsáki, 2011). Moreover, the authors reported a task-dependent increase in γ coherence between the PFC and the VTA during a working memory task (Fujisawa and Buzsáki, 2011). The cellular identity in the VTA accounting for this coupling remained elusive (Fujisawa and Buzsáki, 2011). VTA-GABAergic neurons both receive input from and send reciprocal projections to the PFC (Carr and Sesack, 2000a,b). It is possible that the strong correlation between VTA-GABAergic activation and EEG γ oscillations reflects a top-down cognitive process via transmission of information from the PFC to fast-spiking VTA-GABAergic neurons. Cortical γ band activity is largely generated by fast-spiking GABAergic parvalbumin positive interneurons (Sohal et al., 2009), and is hypothesized to enhance information processing and attention (Womelsdorf et al., 2007). VTA-GABAergic activity may also be related to cortical processes associated with γ activity, such as the temporal representation and consolidation of information (Buzsáki and Draguhn, 2004). Interestingly, our findings suggest that not all behaviors are characterized by this γ coupling, including interactions with same sex conspecifics. Indeed, further studies using projection-specific manipulations could add valuable insights to the functional role of VTA-GABAergic neural activity and their relation to cortical γ oscillations.

Lastly, we observed strong and enduring calcium responses from VTA-GABAergic neurons during the presentation of social stimuli. Specifically, large increases in neuronal activity were observed in response to a novel female mouse, aggressive male intruder, and a four-week-old juvenile mouse, suggesting that population-level VTA-GABAergic responses to social stimuli are independent of valence. In contrast, we observed significantly smaller responses to both positive (high-fat diet) and negative (rat bedding) stimuli that did not involve a social novelty component. It is important to note that we did not control for possible motor aspects of social stimuli. Although Lee et al demonstrated that the firing rate of VTA-GABAergic neurons was phasically modulated by certain movements, such as head orienting, little variation in firing rate was observed during sustained locomotor activity (Lee et al., 2001). VTA neurons, specifically dopaminergic, are associated with social reward and social novelty (Gunaydin et al., 2014; Hung et al., 2017), yet the precise function of VTA-GABAergic neurons in this regard awaits further investigation.

In summary, we demonstrated that VTA-GABAergic neural activity is strongly modulated by arousal state. As many addictive drugs inhibit VTA-GABAergic neurons, resulting in VTA dopaminergic disinhibition (Hyman et al., 2006; Lüscher and Malenka, 2011) a better understanding of VTA microcircuitry in arousal regulation may lead to novel treatments for diseases involving disorders of cortical activation, hyper-arousal, and disrupted sleep.

Acknowledgments

Acknowledgements: We thank the Stanford University laboratory animal facility personnel for providing excellent care to the animals used in these studies.

Footnotes

  • The authors declare no competing financial interests.

  • A.E.-R. is supported by Alfred P. Sloan Foundation, the Frances & Kenneth Eisenberg Translational Research Award, Brain and Behavior Research Foundation Young Investigator Grant 27297, the Kavli Innovators Research Award, and the National Institutes of Health (NIH) Grant 1RF1MH120005-01. J.C.B. is supported by the NIH Grant F32MH115431. W.J.G. is supported by the NIAAA Grant K99 AA025677. L.d.L is supported by the NIH Grants R01 MH087592 and R01 MH102638. This work was also supported by A. Olson and the Stanford Neuroscience Microscopy Service NIH Grant NS069375.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Synthesis

Reviewing Editor: Rae Silver, Columbia University

Decisions are customarily a result of the Reviewing Editor and the peer reviewers coming together and discussing their recommendations until a consensus is reached. When revisions are invited, a fact-based synthesis statement explaining their decision and outlining what is needed to prepare a revision will be listed below. The following reviewer(s) agreed to reveal their identity: Matthew Carter, Barbara Jones.

This manuscript reports the population activity across sleep-wake states and behaviors of the ventral tegmental area (VTA)-GABAergic and also dopaminergic neurons using fiber photometry of GCaMP6 expressing vesicular GABA transporter (VGAT) or tyrosine hydroxylase (TH) expressing neurons. This work extends work from this laboratory demonstrating correlated activity in dopaminergic neurons in the VTA with sleep/wake activity. Together, these experiments seem well done and the results are interesting and impactful to any neuroscientists interested in the VTA system (and its role in reward and learning), and sleep/wake behavior. The experiments are also timely as other studies (Yu et al., 2019 and Chowdhury et al., 2019) recently reported conflicting results about when VTA GABA neurons were most active during NREM and REM states. Restated, questions arise concerning the activity and role of the VTA-GABAergic neurons emerge following the publication of other reports with rather sensational claims concerning a role for the VTA-GABAergic-neurons in promoting NREM sleep and being most active during NREM sleep. The present report which corroborates the previous electrophysiological results and is thus of great importance to be published.

Several changes to the Results could improve the manuscript and data presentation and additions to the Discussion could help to explain the differences between the present study and the three previously published studies.

Major Comments

(1) Several questions were raised about the statistical analyses used in these studies. Please note that the Editor (Christophe Bernard) has written a very thoughtful paper on improving the use of statistical analyses to better understand and support research findings eNeuro. 2019 Aug 1;6(4). Changing the Way We Report, Interpret, and Discuss Our Results to Rebuild Trust in Our Research.

-In the Results, the activity (GCaMP6 fluorescence) of the GABAergic neurons is presented graphically in relation to power of various EEG frequency bands (delta, theta, gamma...) along with the correlation coefficients and probabilities. These relationships are not impressive, even though attaining statistical significance. For the most part, the coefficients indicate that at most, the EEG activity (e.g. gamma) could account for not more than 10% of the variance in neuronal activity within W, NREM or REM.

-“Why not also calculate these relationships across sleep-wake states, during which there is great variation in these EEG frequency bands that should be strongly correlated with the activity of the GABAergic neurons (see e.g. for basal forebrain GABAergic W/PS-active neurons which show average discharge rate as significantly positively correlated with gamma activity with a coefficient of ∼0.60, thus accounting for ∼35% of the variance, Hassani et al., JN, 2010).”

-The more relevant correlations in the current presentation emerge in association with behaviors during waking, and thus greater variations in gamma activity in the waking state condition. (On a minor note in this regard, the data might also be more accessible to the Reader if presented in the text of the Results (e.g., r = 0.33, df= 19,730, p < 0.001) for each relationship mentioned and the graphic representations for theta, delta and sigma might not really be necessary since the correlation coefficients are so minimal, even if statistically significant.)

- It is unclear why a two-way ANOVA is used for analyses of calcium signal and transient rate in Figures 1E,G. Perhaps a one-way ANOVA would be more justified, as it is unnecessary to compare data across sleep states between animals transduced with GCaMP and “control animals” that don’t show any fluctuations.

- Similarly, t-tests don’t seem to be the appropriate tests to use in Figure 1F,H. Because the authors are performing multiple comparisons for each subpanel, a different test should be used or at least multiple comparisons should be accounted for (as they are in Figures 3-5).

(2) It would be helpful if the authors could elaborate further in the Discussion on how other reports found that GABAergic neurons could discharge maximally during NREM sleep. Could it be due to different locations of the probes or to GAD-expressing neurons being different than VGAT-expressing neurons? Moreover, how can these VTA-GABAergic neurons promote NREM sleep if they are active during W and REM? Perhaps through an oscillatory feedback mechanism within a circuit of other cell types? It is the case that from electrophysiological studies, GABAergic neurons appear to be heterogeneous in their discharge profiles in most regions, including the basal forebrain, preoptic area, lateral hypothalamus, and pontomesencephalic tegmentum where W/PS-active neurons are intermingled with SWS-active, PS-active and some W-active GABAergic neurons (see e.g. Jones, Curr Opinion Neurobiol, 2017). Could such heterogeneity be the case in the VTA and would such heterogeneity be totally masked by the measure of population activity with fiber photometry?

Other comments:

(1) In the Methods (p8 line 143), it would be useful for the authors to report how the F signal was converted to dF/F rather than refer readers to a previous paper. Specifically, the authors should be very clear to report what the baseline “F” value represents because VTA GABAergic neurons have a relatively high variance in firing rate as indicated by calcium transience.

(2) Were the fiber optic cables that were connected to the mice attached to the mice for the duration of all experiments? Or were they attached and de-attached to the mice daily? This should be stated in the methods.

(5) In the Methods (p12 line 222) the authors refer to Figure 5f when I believe they mean to refer to Figure 6F.

(6) Speaking of Figure 6F, it is impossible to discern if there is a significant difference between before/during stimulus presentation in the high fat chow and rat bedding conditions. If there is no significant difference, could that be reported in the text or visually depicted in the figure?

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Arousal State-Dependent Alterations in VTA-GABAergic Neuronal Activity
Ada Eban-Rothschild, Jeremy C. Borniger, Gideon Rothschild, William J. Giardino, Joshua G. Morrow, Luis de Lecea
eNeuro 13 February 2020, 7 (2) ENEURO.0356-19.2020; DOI: 10.1523/ENEURO.0356-19.2020

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Arousal State-Dependent Alterations in VTA-GABAergic Neuronal Activity
Ada Eban-Rothschild, Jeremy C. Borniger, Gideon Rothschild, William J. Giardino, Joshua G. Morrow, Luis de Lecea
eNeuro 13 February 2020, 7 (2) ENEURO.0356-19.2020; DOI: 10.1523/ENEURO.0356-19.2020
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Keywords

  • arousal
  • dopamine
  • fiber photometry
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  • VTA

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