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Research ArticleResearch Article: Methods/New Tools, Novel Tools and Methods

Capillary-Based and Stokes-Based Trapping of Serial Sections for Scalable 3D-EM Connectomics

Timothy J. Lee, Mighten C. Yip, Aditi Kumar, Colby F. Lewallen, Daniel J. Bumbarger, R. Clay Reid and Craig R. Forest
eNeuro 24 February 2020, 7 (2) ENEURO.0328-19.2019; DOI: https://doi.org/10.1523/ENEURO.0328-19.2019
Timothy J. Lee
1Georgia Institute of Technology, G. W. Woodruff School of Mechanical Engineering, Atlanta, GA 30332
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Mighten C. Yip
1Georgia Institute of Technology, G. W. Woodruff School of Mechanical Engineering, Atlanta, GA 30332
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Aditi Kumar
1Georgia Institute of Technology, G. W. Woodruff School of Mechanical Engineering, Atlanta, GA 30332
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Colby F. Lewallen
1Georgia Institute of Technology, G. W. Woodruff School of Mechanical Engineering, Atlanta, GA 30332
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Daniel J. Bumbarger
2Allen Institute for Brain Science, Seattle, WA 98109
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R. Clay Reid
2Allen Institute for Brain Science, Seattle, WA 98109
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Craig R. Forest
1Georgia Institute of Technology, G. W. Woodruff School of Mechanical Engineering, Atlanta, GA 30332
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Article Information

DOI 
https://doi.org/10.1523/ENEURO.0328-19.2019
PubMed 
32094293
Published By 
Society for Neuroscience
History 
  • Received August 14, 2019
  • Revision received December 17, 2019
  • Accepted December 18, 2019
  • Published online February 24, 2020.
Copyright & Usage 
Copyright © 2020 Lee et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Author Information

  1. Timothy J. Lee1,
  2. Mighten C. Yip1,
  3. Aditi Kumar1,
  4. Colby F. Lewallen1,
  5. Daniel J. Bumbarger2,
  6. R. Clay Reid2 and
  7. Craig R. Forest1
  1. 1Georgia Institute of Technology, G. W. Woodruff School of Mechanical Engineering, Atlanta, GA 30332
  2. 2Allen Institute for Brain Science, Seattle, WA 98109
  1. Correspondence should be addressed to Timothy J. Lee at timothy.lee{at}gatech.edu.
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Author contributions

  1. Author contributions: T.J.L., R.C.R., and C.R.F. designed research; T.J.L., M.C.Y., A.K., C.F.L., and D.J.B. performed research; D.J.B. contributed unpublished reagents/analytic tools; T.J.L., M.C.Y., A.K., and C.F.L. analyzed data; T.J.L. and M.C.Y. wrote the paper.

Disclosures

  • The authors declare no competing financial interests.

  • This work was supported by the Allen Institute for Brain Science, Seattle, WA; National Institutes of Health Grants 1-U01-MH106027-01 and R01 EY023173; the Intelligence Advanced Research Project Activity Grant D16PC00004; and National Science Foundation Grants EHR 0965945 and CISE 1110947. The Parker H. Petit Institute for Bioengineering and Bioscience and Dr. Robert Nerem provided additional funding via the Nerem International Travel Award.

Funding

  • HHS | National Institutes of Health (NIH)

    1-U01-MH106027-01; R01 EY023173
  • DNI | Intelligence Advanced Research Projects Activity

    D16PC00004
  • National Science Foundation (NSF)

    EHR 0965945; CISE 1110947

Other Version

  • previous version (February 24, 2020).
  • You are viewing the most recent version of this article.

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eneuro: 7 (2)
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Capillary-Based and Stokes-Based Trapping of Serial Sections for Scalable 3D-EM Connectomics
Timothy J. Lee, Mighten C. Yip, Aditi Kumar, Colby F. Lewallen, Daniel J. Bumbarger, R. Clay Reid, Craig R. Forest
eNeuro 24 February 2020, 7 (2) ENEURO.0328-19.2019; DOI: 10.1523/ENEURO.0328-19.2019

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Capillary-Based and Stokes-Based Trapping of Serial Sections for Scalable 3D-EM Connectomics
Timothy J. Lee, Mighten C. Yip, Aditi Kumar, Colby F. Lewallen, Daniel J. Bumbarger, R. Clay Reid, Craig R. Forest
eNeuro 24 February 2020, 7 (2) ENEURO.0328-19.2019; DOI: 10.1523/ENEURO.0328-19.2019
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Keywords

  • capillary interactions
  • electron microscopy
  • histology
  • hydrodynamic
  • serial sectioning
  • ultrastructure

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