Article Figures & Data
Figures
- Figure 1.
Analysis of the distribution of midbrain cholinergic axons in the thalamus. A, Experimental strategy: AAV2–EF1a-DIO-eYFP was injected into the caudal portion of the PPN or the LDT. B, C, YFP-labeled neurons were located at the level of the PPN (B) or the LDT (C). D, E, Selected sections were Nissl-stained to delimit the borders of individual thalamic nuclei and were used to determine the distribution of axons (E) on each nucleus. F, G, Representative coronal confocal images showing the pattern of cholinergic axonal thalamic projections arising in the PPN (F) and LDT (G). Scales bars: 1 mm (B, C), 500 μm (D, E), and 100 μm (F, G).
- Figure 2.
Mapping of midbrain cholinergic axons in individual thalamic nuclei. The spatial distribution of cholinergic axons arising in the PPN (left) or the LDT (right) on each thalamic nucleus was mapped in five selected, Nissl-stained coronal sections along the rostro-caudal axis. Each colored dot represents a 60 × 80 μm grid that contained YFP-positive axons.
- Figure 3.
Axonal distribution of PPN and LDT cholinergic axons in the thalamus. Histogram showing the comparison of the distribution density of PPN (n = 3) and LDT (n = 3) YFP-transduced cholinergic axons. Data are expressed as the percentage of the total area occupied by labeled axons (defined by the counting grid) for each of the thalamic structures (mean ± SEM).
- Figure 4.
Quantification of midbrain cholinergic synapses in the thalamus. A–D, Nissl-stained sections from animals injected with AAV-FLEX-Synaptophysin-mRuby were used to quantify, in YFP-labeled axons (B) and their varicosities (arrows), the number of synaptophysin particles (C, D) across selected thalamic nuclei. E, The number of synaptophysin particles was determined on 11 selected thalamic nuclei and is represented as the percentage of synaptophysin particles in each nucleus out of the total number of particles found in all selected nuclei included in this analysis. The values of the two animals included in the analysis (circles) are shown individually. Scale bar: 25 μm.
Tables
Abbreviation Structure Abbreviation Structure AD Anterior dorsal MDL Mediodorsal lateral AM Anterior medial MDM Medial dorsal medial AMV Anterio medio-ventral MG Medial geniculate Ang Angular MGD Medial geniculate dorsal AV Anterioventral MGV Medial geniculate ventral AVDM Anterioventral dorsomedial MHb Medial habenula AVVL Anterioventral ventrolateral OPC Oval paracentral CL Central lateral PC Paracentral CM Central medial Pf Parafascicular DLG Dorsolateral geniculate PO Posterior nucleus IAD Interanterio-dorsal PPN Pedunculopontine IAM Interanterio-medial PT Paretenial ID Intermediodorsal PV Paraventricular LHb Lateral habenula PV Ant Paraventricular anterior LHbM Lateral habenula medial PVPo Paraventricular posterior LHbL Lateral habenula lateral Re Reuniens LDDM Lateral dorsal dorsomedial Rh Rhomboid LDVL Lateral dorsal ventrolateral Rt Reticular thalamic LDT Laterodorsal tegmental VA Ventral anterior LPLC Lateral posterior laterocentral VL Ventral lateral LPLR Lateral posterior laterorostral VLG Ventrolateral geniculate LPMC Lateral posterior mediocentral VM Ventral medial LPMR Lateral posterior mediorostral VPL Ventral posterolateral MD Mediodorsal VPM Ventral posteromedial MDC Mediodorsal central VPPC Ventral posterior parvicellular
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