Extended Data Figure 6-2
Gene expression of the neurohypophyseal and IL. A, A brightfield image of a horizontal section of adult mouse pituitary showing the locations of the NH, IL, and AH. The white boxes in the brightfield image mark the locations of specific pituitary subdomains shown in the fluorescent images below (scale bar, 100 µm). B, Different fields of view (marked by roman numbers) of horizontal section (7 µm) of pituitaries derived from three-month-old Cx3cr1-GFP macrophage/microglia transgenic reporter mouse, which were subjected to smFISH. The separate image panels show the expression of the pituicyte marker Srebf1 and epithelial-like marker Cyp2f2 in the NH (I) and the epithelial-like marker Lcn2 at the boundary of IL and AH (II), also marked by the white dotted line (scale bars, 20 µm). C, Confocal Z-stack (maximum intensity projection) of horizontal vibratome pituitary section (50 µm) from a three-month-old male mouse harboring the transgenic macrophage/microglia reporter Cx3cr1-GFP (scale bar, 200 µm). D, Immunostaining of the T-cell marker, Cd3, showing a lone T-cell inside the NH horizontal pituitary cryosection (7 µm) of a three-month-old C57/BL6 male mouse (scale bars, 20 µm). Download Figure 6-2, TIF file.