Figure 2. miR-137 antagomir infusion directly into the CeA reverses AIE-induced anxiety-like behaviors and target mRNA expression. A, Schematic of AIE or adolescent intermittent saline (AIS) exposure followed by cannulation surgery and infusion of an antagomir specific to miR-137, scrambled antagomir, or vehicle directly into the CeA of adult male rats. B, Bar diagram showing anxiety-like behaviors (measured in the EPM test) in AIS and AIE adult rats infused with miR-137 antagomir, scrambled antagomir, or vehicle (iFect solution) directly into the CeA (n = 6–7, ***p < 0.001 vs AIS+ vehicle, ###p < 0.001 vs AIE+ vehicle, $$$p < 0.001 vs AIE+ scrambled by one-way ANOVA followed by Tukey’s post hoc test). C, qPCR analysis of the miR-137 target genes Lsd1 and Lsd1 + 8a mRNA in the amygdala of AIS and AIE adult rats infused with miR-137 antagomir, scrambled antagomir, or vehicle directly into the CeA (n = 6–7, *p < 0.05 vs AIS+ vehicle, **p < 0.01 vs AIS+ vehicle, ##p < 0.01 vs AIE+ vehicle, $p < 0.05 vs AIE+ scrambled by one-way ANOVA followed by Tukey’s post hoc test). D, ChIP analysis of LSD1 occupancy of the Bdnf exon IV promoter region in the amygdala of AIS and AIE adult rats infused with miR-137 antagomir, scrambled antagomir, or vehicle into the CeA (n = 6–7, *p < 0.05, **p < 0.01 vs AIS+ vehicle by one-way ANOVA followed by Tukey’s post hoc test). E, ChIP analysis of H3K9me2 and H3K4me2 occupancy of the Bdnf exon IV promoter region in the amygdala of AIS and AIE adult rats infused with miR-137 antagomir, scrambled antagomir, or vehicle into the CeA (n = 6–7, *p < 0.05 vs AIS+ vehicle, ##p < 0.01 vs AIE+ vehicle, $p < 0.05 vs AIE+ scrambled by one-way ANOVA followed by Tukey’s post hoc test). F, qPCR analysis of Bdnf exon IV (Bdnf IV) mRNA in the amygdala of AIS- and AIE-exposed adult rats infused with miR-137 antagomir, scrambled antagomir, or vehicle (iFect solution) into the CeA (n = 6–7, *p < 0.05 vs AIS+ vehicle by one-way ANOVA followed by Tukey’s post hoc test). Data expressed as mean ± SEM.