Figure 1.
Cannula placements, test design, and FR1 data. A, Cannula placements for FR1 cohort. Dorsal mPFC cannula placements (triangles) and ventral mPFC cannula placements (circles). Numbers are A/P distance from bregma. B, Histology of coronal slices stained with neutral red showing cannula tracks for dorsal (top) and ventral (bottom) mPFC. C, Timeline for FR1 testing. Rats were retrained for 3–10 d after surgery. They then received sham infusions followed by 8 d of FR1 tests. Rats received one of four infusions every other day of testing: bilateral inactivation, bilateral aCSF, unilateral left, or right inactivation, counterbalanced across rats. All rats received all four conditions. aCSF (stripes) = control infusion, BI (solid) = bilateral inactivation, LI (dots) = inactivation of left hemisphere, RI (checkers) = inactivation of right hemisphere. D, F, Total number of nose pokes, time-out nose pokes, and initiated trials. E, G, Total number of well entries, non-rewarded well entries, and rewarded well entries. D, E, There was a significant increase in total number of nose pokes and total number of rewarded well entries when the dorsal mPFC was bilaterally inactivated (*). F, G, Ventral mPFC inactivation did not affect nose poking or well entries; *p < 0.05, Dunnett’s test for planned multiple comparison.