Figure 2C | EB size (EB) | Dunnett’s test | Control vs BP1 | 0.4406 |
| | | Control vs SCZ1 | 0.5982 |
| EB size (DSi-EB) | Dunnett’s test | Control vs BP1 | 0.5619 |
| | | Control vs SCZ1 | 0.9784 |
Figure 2D | EB form factor (EB) | Dunnett’s test | Control vs BP1 | 0.5015 |
| | | Control vs SCZ1 | 0.7476 |
| EB form factor (DSi-EB) | Dunnett’s test | Control vs BP1 | 0.0662 |
| | | Control vs SCZ1 | 0.0562 |
Figure 2E | PCDH15 gene expression | Dunnett’s test | Control vs BP1 | 0.3861 |
| | | Control vs SCZ1 | 0.3897 |
| Reln gene expression | | Control vs BP1 | 0.7288 |
| | | Control vs SCZ1 | 0.5178 |
Figure 2G | Intensity/area (βIII tubulin) | Dunnett’s test | Control vs BP1 | 0.9826 |
| | | Control vs SCZ1 | 0.2543 |
| Intensity/area (αSMA) | Dunnett’s test | Control vs BP1 | 0.9778 |
| | | Control vs SCZ1 | 0.9238 |
| Intensity/area (AFP) | Dunnett’s test | Control vs BP1 | 0.7731 |
| | | Control vs SCZ1 | 0.9345 |
Figure 2J | Neurite length (day 10) | Dunnett’s test | Control vs BP1 | 0.7746 |
| | | Control vs SCZ1 | 0.8824 |
| Neurite length (day13) | Dunnett’s test | Control vs BP1 | 0.0293 |
| | | Control vs SCZ1 | 0.0324 |
| Neurite length (day16) | Dunnett’s test | Control vs BP1 | 0.0192 |
| | | Control vs SCZ1 | 0.01 |
| Neurite length (day22) | Dunnett’s test | Control vs BP1 | 0.0036 |
| | | Control vs SCZ1 | 0.0025 |
Figure 3C | No. of cells (βIII tubulin+/HO+) | Tukey’s test | 1210B2 vs 201B7 | 0.9993 |
| | | 1210B2 vs BP1-1 | 1 |
| | | 1210B2 vs BP1-2 | 1 |
| | | 1210B2 vs BP2-1 | 0.0218 |
| | | 1210B2 vs SCZ1-1 | 0.5216 |
| | | 1210B2 vs SCZ1-2 | 0.875 |
| | | 201B7 vs BP1-1 | 0.9975 |
| | | 201B7 vs BP1-2 | 0.9989 |
| | | 201B7 vs BP2-1 | 0.0036 |
| | | 201B7 vs SCZ1-1 | 0.2079 |
| | | 201B7 vs SCZ1-2 | 0.5352 |
| | | BP1-1 vs BP1-2 | 1 |
| | | BP1-1 vs BP2-1 | 0.0077 |
| | | BP1-1 vs SCZ1-1 | 0.4016 |
| | | BP1-1 vs SCZ1-2 | 0.8143 |
| | | BP1-2 vs BP2-1 | 0.0042 |
| | | BP1-2 vs SCZ1-1 | 0.3116 |
| | | BP1-2 vs SCZ1-2 | 0.7283 |
| | | BP2-1 vs SCZ1-1 | 0.5238 |
| | | BP2-1 vs SCZ1-2 | 0.1306 |
| | | SCZ1-1 vs SCZ1-2 | 0.9847 |
| No. of cells (MAP2+/βIII tubulin+) | Tukey’s test | 1210B2 vs 201B7 | 1 |
| | | 1210B2 vs BP1-1 | 0.6317 |
| | | 1210B2 vs BP1-2 | 0.9984 |
| | | 1210B2 vs BP2-1 | 0.6973 |
| | | 1210B2 vs SCZ1-1 | 0.9988 |
| | | 1210B2 vs SCZ1-2 | 0.9994 |
| | | 201B7 vs BP1-1 | 0.7153 |
| | | 201B7 vs BP1-2 | 1 |
| | | 201B7 vs BP2-1 | 0.7806 |
| | | 201B7 vs SCZ1-1 | 1 |
| | | 201B7 vs SCZ1-2 | 1 |
| | | BP1-1 vs BP1-2 | 0.7807 |
| | | BP1-1 vs BP2-1 | 1 |
| | | BP1-1 vs SCZ1-1 | 0.8539 |
| | | BP1-1 vs SCZ1-2 | 0.7703 |
| | | BP1-2 vs BP2-1 | 0.8448 |
| | | BP1-2 vs SCZ1-1 | 1 |
| | | BP1-2 vs SCZ1-2 | 1 |
| | | BP2-1 vs SCZ1-1 | 0.8955 |
| | | BP2-1 vs SCZ1-2 | 0.8325 |
| | | SCZ1-1 vs SCZ1-2 | 1 |
|
| No. of cells (VGluT2+βIII tubulin+/βIII tubulin+) | Tukey’s test | 1210B2 vs 201B7 | 0.9629 |
| | | 1210B2 vs BP1-1 | 0.2892 |
| | | 1210B2 vs BP1-2 | 0.9885 |
| | | 1210B2 vs BP2-1 | 0.8416 |
| | | 1210B2 vs SCZ1-1 | 0.6553 |
| | | 1210B2 vs SCZ1-2 | 0.9756 |
| | | 201B7 vs BP1-1 | 0.7818 |
| | | 201B7 vs BP1-2 | 0.9999 |
| | | 201B7 vs BP2-1 | 0.9996 |
| | | 201B7 vs SCZ1-1 | 0.9859 |
| | | 201B7 vs SCZ1-2 | 1 |
| | | BP1-1 vs BP1-2 | 0.5003 |
| | | BP1-1 vs BP2-1 | 0.9484 |
| | | BP1-1 vs SCZ1-1 | 0.9956 |
| | | BP1-1 vs SCZ1-2 | 0.6512 |
| | | BP1-2 vs BP2-1 | 0.9885 |
| | | BP1-2 vs SCZ1-1 | 0.9093 |
| | | BP1-2 vs SCZ1-2 | 1 |
| | | BP2-1 vs SCZ1-1 | 0.9998 |
| | | BP2-1 vs SCZ1-2 | 0.9975 |
| | | SCZ1-1 vs SCZ1-2 | 0.9616 |
Figure 3D | PCDH15 gene expression (iPS) | Dunnett’s test | Control vs BP | 0.9527 |
| | | Control vs SCZ | 0.9354 |
| PCDH15 gene expression (neuron) | Dunnett’s test | Control vs BP | 0.2862 |
| | | Control vs SCZ | 0.4102 |
| RELN gene expression (iPS) | Dunnett’s test | Control vs BP | 0.1826 |
| | | Control vs SCZ | 0.3746 |
| RELN gene expression (neuron) | Dunnett’s test | Control vs BP | 0.6918 |
| | | Control vs SCZ | 0.9941 |
Figure 3G | No. of cells (βIII tubulin+/HO+) | Tukey’s test | 1210B2 vs 201B7 | 0.98 |
| | | 1210B2 vs BP1-1 | 0.9914 |
| | | 1210B2 vs BP1-2 | 0.9863 |
| | | 1210B2 vs BP2-1 | 0.4556 |
| | | 1210B2 vs SCZ1-1 | 1 |
| | | 1210B2 vs SCZ1-2 | 0.9992 |
| | | 201B7 vs BP1-1 | 0.7241 |
| | | 201B7 vs BP1-2 | 1 |
| | | 201B7 vs BP2-1 | 0.1395 |
| | | 201B7 vs SCZ1-1 | 0.9894 |
| | | 201B7 vs SCZ1-2 | 0.9993 |
| | | BP1-1 vs BP1-2 | 0.7192 |
| | | BP1-1 vs BP2-1 | 0.7873 |
| | | BP1-1 vs SCZ1-1 | 0.9536 |
| | | BP1-1 vs SCZ1-2 | 0.8677 |
| | | BP1-2 vs BP2-1 | 0.1102 |
| | | BP1-2 vs SCZ1-1 | 0.9937 |
| | | BP1-2 vs SCZ1-2 | 0.9998 |
| | | BP2-1 vs SCZ1-1 | 0.2366 |
| | | BP2-1 vs SCZ1-2 | 0.1646 |
| | | SCZ1-1 vs SCZ1-2 | 0.9999 |
| No. of cells (MAP2+/βIII tubulin+) | Tukey’s test | 1210B2 vs 201B7 | 0.9997 |
| | | 1210B2 vs BP1-1 | 0.9897 |
| | | 1210B2 vs BP1-2 | 0.9829 |
| | | 1210B2 vs BP2-1 | 0.5806 |
| | | 1210B2 vs SCZ1-1 | 0.9812 |
| | | 1210B2 vs SCZ1-2 | 0.9842 |
| | | 201B7 vs BP1-1 | 1 |
| | | 201B7 vs BP1-2 | 0.9998 |
| | | 201B7 vs BP2-1 | 0.8884 |
| | | 201B7 vs SCZ1-1 | 0.9037 |
| | | 201B7 vs SCZ1-2 | 0.9149 |
| | | BP1-1 vs BP1-2 | 1 |
| | | BP1-1 vs BP2-1 | 0.902 |
| | | BP1-1 vs SCZ1-1 | 0.6069 |
| | | BP1-1 vs SCZ1-2 | 0.6541 |
| | | BP1-2 vs BP2-1 | 0.9712 |
| | | BP1-2 vs SCZ1-1 | 0.6265 |
| | | BP1-2 vs SCZ1-2 | 0.6636 |
| | | BP2-1 vs SCZ1-1 | 0.0941 |
|
| | | BP2-1 vs SCZ1-2 | 01225 |
| | | SCZ1-1 vs SCZ1-2 | 1 |
| No. of cells (GABA+βIII tubulin+/βIII tubulin+) | Tukey’s test | 1210B2 vs 201B7 | 0.9415 |
| | | 1210B2 vs BP1-1 | 0.2961 |
| | | 1210B2 vs BP1-2 | 0.6938 |
| | | 1210B2 vs BP2-1 | 0.2707 |
| | | 1210B2 vs SCZ1-1 | 0.343 |
| | | 1210B2 vs SCZ1-2 | 0.8786 |
| | | 201B7 vs BP1-1 | 0.9599 |
| | | 201B7 vs BP1-2 | 0.9994 |
| | | 201B7 vs BP2-1 | 0.949 |
| | | 201B7 vs SCZ1-1 | 0.9747 |
| | | 201B7 vs SCZ1-2 | 1 |
| | | BP1-1 vs BP1-2 | 0.9981 |
| | | BP1-1 vs BP2-1 | 1 |
| | | BP1-1 vs SCZ1-1 | 1 |
| | | BP1-1 vs SCZ1-2 | 0.9345 |
| | | BP1-2 vs BP2-1 | 0.9967 |
| | | BP1-2 vs SCZ1-1 | 0.9994 |
| | | BP1-2 vs SCZ1-2 | 0.9993 |
| | | BP2-1 vs SCZ1-1 | 1 |
| | | BP2-1 vs SCZ1-2 | 0.9169 |
| | | SCZ1-1 vs SCZ1-2 | 0.9587 |
Figure 3H | PCDH15 gene expression (iPS) | Dunnett’s test | Control vs BP | 0.2334 |
| | | Control vs SCZ1 | 0.8567 |
| PCDH15 gene expression (neuron) | Dunnett’s test | Control vs BP | 0.3458 |
| | | Control vs SCZ | 0.5146 |
| RELN gene expression (iPS) | Dunnett’s test | Control vs BP | 0.999 |
| | | Control vs SCZ | 0.9874 |
| RELN gene expression (neuron) | Dunnett’s test | Control vs BP | 0.081 |
| | | Control vs SCZ | 0.0663 |
Figure 5D | Dendrite length | Dunnett’s test | Control vs BP | 0.0001 |
| | | Control vs SCZ | 0.0002 |
Figure 5E | Homer I puncta No. | Dunnett’s test | Control vs BP | 0.0093 |
| | | Control vs SCZ | 0.009 |
| Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.0067 |
| | | Control vs SCZ | 0.0014 |
| Homer I-Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.0182 |
| | | Control vs SCZ | 0.0117 |
Figure 5H | Dendrite length | Dunnett’s test | Control vs BP | 0.004 |
| | | Control vs SCZ | 0.0016 |
Figure 5I | Gephyrin puncta No. | Dunnett’s test | Control vs BP | 0.0065 |
| | | Control vs SCZ | 0.0048 |
| Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.0173 |
| | | Control vs SCZ | 0.0099 |
| Gephyrin-Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.0044 |
| | | Control vs SCZ | 0.0042 |
Figure 6E | Gene expression of PCDH15 | Student’s t test | Control 1 vs PCDH15del (glutamatergic neurons) | 0.1757 |
| | | Control 1 vs PCDH15del (GABAergic neurons) | 0.01 |
Figure 6G | Gene expression of RELN | Student’s t test | Control 2 vs RELNdel (glutamatergic neurons) | <0.0001 |
| | | Control 2 vs RELNdel (GABAergic neurons) | 0.0014 |
Figure 7B | No. of cells (βIII tubulin+/HO+) | Student’s t test | Control 1 vs PCDH15del | 0.1781 |
| | | Control 1 vs RELNdel | 0.2969 |
| No. of cells (MAP2+/βIII tubulin+) | Student’s t test | Control 1 vs PCDH15del | 0.0003 |
| | | Control 1 vs RELNdel | 0.2568 |
| No. of cells (VGluT2+βIII tubulin+/βIII tubulin+) | Student’s t test | Control 1 vs PCDH15del | 0.0098 |
| | | Control 1 vs RELNdel | 0.2754 |
Figure 7C | Dendrite length | Student’s t test | Control 1 vs PCDH15del | 0.0418 |
| | | Control 1 vs RELNdel | 0.063 |
Figure 7D | Homer I puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.0282 |
| | | Control 1 vs RELNdel | 0.0209 |
| Synapsin I puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.2101 |
| | | Control 1 vs RELNdel | 0.3278 |
| Homer I-Synapsin I puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.982 |
| | | Control 1 vs RELNdel | 0.7786 |
Figure 7F | No. of cells (βIII tubulin+/HO+) | | Control 1 vs PCDH15del | 0.1441 |
| | | Control 1 vs RELNdel | 0.1954 |
| No. of cells (MAP2+/βIII tubulin+) | | Control 1 vs PCDH15del | 0.0281 |
| | | Control 1 vs RELNdel | 0.4007 |
|
| No. of cells (GABA+βIII tubulin+/βIII tubulin+) | | Control 1 vs PCDH15del | 0.1269 |
| | | Control 1 vs RELNdel | 0.66 |
Figure 7G | Dendrite length | Student’s t test | Control 1 vs PCDH15del | 0.0055 |
| | | Control 1 vs RELNdel | 0.9907 |
Figure 7H | Gephyrin puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.553 |
| | | Control 1 vs RELNdel | 0.0452 |
| Synapsin I puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.2637 |
| | | Control 1 vs RELNdel | 0.0431 |
| Gephyrin-Synapsin I puncta No. | Student’s t test | Control 1 vs PCDH15del | 0.5136 |
| | | Control 1 vs RELNdel | 0.3123 |
Figure 8B | Dendrite length (Glutamatergic neurons) | Dunnett’s test | Control vs BP | 0.0001 |
| | | Control vs SCZ | 0.0002 |
| Homer I puncta No. | Dunnett’s test | Control vs BP | 0.0003 |
| | | Control vs SCZ | 0.001 |
| Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.0011 |
| | | Control vs SCZ | 0.0012 |
| Homer I-Synapsin I puncta No. | Dunnett’s test | Control vs BP | <0.0001 |
| | | Control vs SCZ | <0.0001 |
| Dendrite length (GABAergic neurons) | Dunnett’s test | Control vs BP | 0.0021 |
| | | Control vs SCZ | 0.0027 |
| Gephyrin puncta No. | Dunnett’s test | Control vs BP | 0.0002 |
| | | Control vs SCZ | 0.0011 |
| Synapsin I puncta No. | Dunnett’s test | Control vs BP | 0.1728 |
| | | Control vs SCZ | 0.1023 |
| Gephyrin-Synapsin I puncta No. | Dunnett’s test | Control vs BP | <0.0001 |
| | | Control vs SCZ | <0.0001 |
Figure 8E | Spike frequency | Dunnett’s test | Control vs BP (day28) | 0.8839 |
| | | Control vs SCZ (day28) | 0.924 |
| | | Control vs BP (day42) | 0.7486 |
| | | Control vs SCZ (day42) | 0.9812 |
| | Paired t test | day28 vs day42 | 0.0104 |
Figure 8F | Spike frequency | Dunnett’s test | Control 1 vs PCDH15del (day28) | 0.6129 |
| | | Control 2 vs RELNdel (day28) | 0.7575 |
| | | Control 1 vs PCDH15del (day42) | 0.2678 |
| | | Control 2 vs RELNdel (day42) | 0.9187 |
| | Paired t test | day28 vs day42 | 0.0483 |
Figure 8G | Spike number ratio (CNQX) | Dunnett’s test | Control vs BP | 0.0351 |
| | | Control vs SCZ | 0.0479 |
| Spike number ratio (AP-5) | | Control vs BP | 0.1922 |
| | | Control vs SCZ | 0.1165 |
| Spike number ratio (GABA) | | Control vs BP | 0.1204 |
| | | Control vs SCZ | 0.0071 |
Figure 8I | ΔFmax | Dunnett’s test | 1210B2 vs BP1-1 | 0.9215 |
| | | 1210B2 vs BP1-2 | 0.9233 |
| | | 1210B2 vs BP2-1 | 1 |
| | | 1210B2 vs SCZ1-1 | 0.9999 |
| | | 1210B2 vs SCZ1-2 | 0.9997 |
| Spike frequency | Dunnett’s test | 1210B2 vs BP1-1 | 0.2334 |
| | | 1210B2 vs BP1-2 | 0.7435 |
| | | 1210B2 vs BP2-1 | 0.9984 |
| | | 1210B2 vs SCZ1-1 | 0.9985 |
| | | 1210B2 vs SCZ1-2 | 1 |
Figure 8J | ΔFmax | Student’s t test | Control 1 vs PCDH15del | 0.7346 |
| | | Control 1 vs RELNdel | 0.5067 |
| Spike frequency | Student’s t test | Control 1 vs PCDH15del | 0.0795 |
| | | Control 1 vs RELNdel | 0.3771 |