Nest width | Three-way rmANOVA | Genotype | F(2,58) = 4.07 | *p = 0.0222 |
(Fig. 5A) | | Time | F(2,116) = 19.12 |
*p < 0.0001 |
| | Sex | F(1,58) = 0.26 | p = 0.6119 |
| | Genotype × time | F(4,116) = 0.67 | p = 0.6162 |
| Tukey HSD | Shank3+/+CreTam− vs | Shank3+/GCreTam− | p = 0.5894 |
| | Shank3+/+CreTam− vs | Shank3G/GCreTam− |
*p = 0.0089 |
| | Shank3+/GCreTam− vs | Shank3G/GCreTam− | p = 0.1515 |
Nest height | Three-way rmANOVA | Genotype | F(2,58) = 3.38 |
*p = 0.0408 |
(Fig. 5B) | | Time | F(2,116) = 33.32 |
*p < 0.0001 |
| | Sex | F(1,58) = 0.01 | p = 0.9336 |
| | Genotype × time | F(4,116) = 0.46 | p = 0.7621 |
| | Genotype × sex | F(2,58) = 1.82 | p = 0.1710 |
| Tukey HSD | Shank3+/+CreTam− vs | Shank3+/GCreTam− | p = 0.9865 |
| | Shank3+/+CreTam− vs | Shank3G/GCreTam− |
*p = 0.0392 |
| | Shank3+/GCreTam− vs | Shank3G/GCreTam− |
*p = 0.0473 |
Marble burying | Two-way ANOVA | Genotype | F(2,58) = 12.30 |
*p < 0.0001 |
(Fig. 5C) | | Sex | F(1,58) = 0.13 | p = 0.7208 |
| | Genotype × time | F(4,116) = 0.67 | p = 0.6162 |
| Tukey HSD | Shank3+/+CreTam− vs | Shank3+/GCreTam− | p = 0.4965 |
| | Shank3+/+CreTam− vs | Shank3G/GCreTam− |
*p = 0.0001 |
| | Shank3+/GCreTam− vs | Shank3G/GCreTam− |
*p = 0.0017 |
Locomotor | Three-way rmANOVA | Genotype | F(2,58) = 0.47 | p = 0.6274 |
(Fig. 5D) | | Time | F(23,1334) = 103.60 |
*p < 0.0001 |
| | Sex | F(1,58) = 0.82 | p = 0.3689 |
| | Genotype × time | F(46,1334) = 3.37 |
*p < 0.0001 |
| | Genotype × sex | F(2,58) = 0.37 | p = 0.6893 |
| Tukey HSD | Shank3+/+CreTam− vs | Shank3+/GCreTam− | p = 0.9828 |
| | Shank3+/+CreTam− vs | Shank3G/GCreTam− | p = 0.5155 |
| | Shank3+/GCreTam− vs | Shank3G/GCreTam− | p = 0.6796 |
Rotarod | Three-way rmANOVA | Genotype | F(2,58) = 5.83 |
*p = 0.0049 |
(Fig. 5E) | | Trial | F(7,406) = 13.89 |
*p < 0.0001 |
| | Sex | F(1,58) = 4.31 |
*p = 0.0424 |
| | Genotype × trial | F(14,406) = 1.87 |
*p = 0.0282 |
| | Genotype × sex | F(2,58) = 0.94 | p = 0.3970 |
| Tukey HSD | Shank3+/+CreTam− vs | Shank3+/GCreTam− | p = 0.4183 |
| | Shank3+/+CreTam− vs | Shank3G/GCreTam− |
*p = 0.0015 |
| | Shank3+/GCreTam− vs | Shank3G/GCreTam− | p = 0.4183 |
fEPSP slope | Two-way rmANOVA | Genotype | F(1,17) = 102.42 |
*p < 0.0001 |
(Fig. 5F) | | Intensity | F(7,119) = 36.51 |
*p < 0.0001 |
| | Genotype × intensity | F(7,119) = 2.90 |
*p = 0.0078 |
Fiber volley | Two-way rmANOVA | Genotype | F(1,8) = 5.12 | p = 0.0535 |
| | Intensity | F(7,56) = 69.13 |
*p < 0.0001 |
| | Genotype × intensity | F(7,56) = 3.51 |
*p = 0.0034 |
Tamoxifen-treated Shank3GCreTam− |
Nest width | Three-way rmANOVA | Genotype | F(2,54) = 5.09 |
*p = 0.0047 |
(Fig. 6A) | | Time | F(2,108) = 23.28 |
*p < 0.0001 |
| | Sex | F(1,54) = 0.31 | p = 0.5813 |
| | Genotype × time | F(4,108) = 1.12 | p = 0.3527 |
| | Genotype × sex | F(2,54) = 0.35 | p = 0.7070 |
| Tukey HSD | Shank3+/+CreTam− vs | Shank3+/GCreTam− | p = 0.3842 |
| | Shank3+/+CreTam− vs | Shank3G/GCreTam− |
*p = 0.0068 |
| | Shank3+/GCreTam− vs | Shank3G/GCreTam− | p = 0.1757 |
Nest height | Three-way rmANOVA | Genotype | F(2,54) = 2.47 | p = 0.0942 |
(Fig. 6B) | | Time | F(2,108) = 39.18 |
*p < 0.0001 |
| | Sex | F(1,54) = 0.01 | p = 0.9046 |
| | Genotype × time | F(4,108) = 1.07 | p = 0.3753 |
| | Genotype × sex | F(2,54) = 0.14 | p = 0.8719 |
| Tukey HSD | Shank3+/+CreTam− vs | Shank3+/GCreTam− | p = 0.4260 |
| | Shank3+/+CreTam− vs | Shank3G/GCreTam− | p = 0.0811 |
| | Shank3+/GCreTam− vs | Shank3G/GCreTam− | p = 0.6415 |
|
Marble burying | Two-way ANOVA | Genotype | F(2,54) = 22.52 |
*p < 0.0001 |
(Fig. 6C) | | Sex | F(1,54) = 0.86 | p = 0.3575 |
| | Genotype × sex | F(2,54) = 0.20 | p = 0.8195 |
| Tukey HSD | Shank3+/+CreTam− vs | Shank3+/GCreTam− | p = 0.0776 |
| | Shank3+/+CreTam− vs | Shank3G/GCreTam− |
*p = 0.0001 |
| | Shank3+/GCreTam− vs | Shank3G/GCreTam− |
*p = 0.0003 |
Locomotor | Three-way rmANOVA | Genotype | F(2,54) = 5.20 |
*p = 0.0086 |
(Fig. 6D) | | Time | F(23,1242) = 96.67 |
*p < 0.0001 |
| | Sex | F(1,54) = 0.56 | p = 0.4570 |
| | Genotype × time | F(46,1242) = 4.55 |
*p < 0.0001 |
| | Genotype × sex | F(2,54) = 0.11 | p = 0.8928 |
| Tukey HSD | Shank3+/+CreTam− vs | Shank3+/GCreTam− | p = 0.0740 |
| | Shank3+/+CreTam− vs | Shank3G/GCreTam− |
*p = 0.0097 |
| | Shank3+/GCreTam− vs | Shank3G/GCreTam− | p = 0.6566 |
Rotarod | Three-way rmANOVA | Genotype | F(2,54) = 13.62 |
*p < 0.0001 |
(Fig. 6E) | | Trial | F(7,378) = 16.98 |
*p < 0.0001 |
| | Sex | F(1,54) = 17.21 |
*p = 0.0001 |
| | Genotype × trial | F(14,378) = 2.79 |
*p = 0.0006 |
| | Genotype × sex | F(2,54) = 2.50 | p = 0.0912 |
| Tukey HSD | Shank3+/+CreTam− vs | Shank3+/GCreTam− |
*p = 0.0253 |
| | Shank3+/+CreTam− vs | Shank3G/GCreTam− |
*p = 0.0001 |
| | Shank3+/GCreTam− vs | Shank3G/GCreTam− |
*p = 0.0346 |
fEPSP slope | Two-way rmANOVA | Genotype | F(1,40) = 8.33 |
*p = 0.0063 |
(Fig. 6F) | | Intensity | F(7,280) = 113.08 |
*p < 0.0001 |
| | Genotype × intensity | F(7,280) = 9.94 |
*p < 0.0001 |
Fiber volley | Two-way rmANOVA | Genotype | F(1,19) = 0.49 | p = 0.4931 |
| | Intensity | F(7,133) = 51.55 |
*p < 0.0001 |
| | Genotype × intensity | F(7,133) = 0.69 | p = 0.6768 |
Tamoxifen-treated Shank3GCreTam+ mice |
Nest width | Three-way rmANOVA | Genotype | F(2,50) = 0.30 | p = 0.7405 |
(Fig. 7A) | | Time | F(2,100) = 28.50 |
*p < 0.0001 |
| | Sex | F(1,50) = 0.62 | p = 0.4336 |
| | Genotype × time | F(4,100) = 1.12 | p = 0.3531 |
| | Genotype × sex | F(2,50) = 0.22 | p = 0.8038 |
| Tukey HSD | Shank3+/+CreTam+ vs | Shank3+/GCreTam+ | p = 0.9947 |
| | Shank3+/+CreTam+ vs | Shank3G/GCreTam+ | p = 0.7240 |
| | Shank3+/GCreTam+ vs | Shank3G/GCreTam+ | p = 0.8221 |
Nest height | Three-way rmANOVA | Genotype | F(2,50) = 0.07 | p = 0.9335 |
(Fig. 7B) | | Time | F(2,100) = 35.62 |
*p < 0.0001 |
| | Sex | F(1,50) = 0.58 | p = 0.4490 |
| | Genotype × time | F(4,100) = 0.48 | p = 0.7507 |
| | Genotype × sex | F(2,50) = 0.17 | p = 0.8425 |
| Tukey HSD | Shank3+/+CreTam+ vs | Shank3+/GCreTam+ | p = 0.9623 |
| | Shank3+/+CreTam+ vs | Shank3G/GCreTam+ | p = 0.9767 |
| | Shank3+/GCreTam+ vs | Shank3G/GCreTam+ | p = 0.9000 |
Marble burying | Two-way ANOVA | Genotype | F(2,48) = 3.68 |
*p = 0.0326 |
(Fig. 7C) | | Sex | F(1,48) = 0.00 | p = 0.9634 |
| | Genotype × sex | F(2,48) = 0.26 | p = 0.7688 |
| Tukey HSD | Shank3+/+CreTam+ vs | Shank3+/GCreTam+ | p = 0.6972 |
| | Shank3+/+CreTam+ vs | Shank3G/GCreTam+ |
*p = 0.0296 |
| | Shank3+/GCreTam+ vs | Shank3G/GCreTam+ | p = 0.6972 |
Locomotor | Three-way rmANOVA | Genotype | F(2,50) = 2.12 | p = 0.1311 |
(Fig. 7D) | | Time | F(23,1150) = 99.68 |
*p < 0.0001 |
| | Sex | F(1.50) = 0.49 | p = 0.4866 |
| | Genotype × time | F(46,1150) = 1.82 |
*p < 0.0008 |
| | Genotype × sex | F(2,50) = 0.03 | p = 0.9701 |
| Tukey HSD | Shank3+/+CreTam+ vs | Shank3+/GCreTam+ | p = 0.8310 |
| | Shank3+/+CreTam+ vs | Shank3G/GCreTam+ | p = 0.1097 |
| | Shank3+/GCreTam+ vs | Shank3G/GCreTam+ | p = 0.4023 |
|
Rotarod | Three-way rmANOVA | Genotype | F(2,56) = 0.12 | p = 0.8894 |
(Fig. 7E) | | Trial | F(7,392) = 17.89 |
*p < 0.0001 |
| | Sex | F(1,56) = 4.46 |
*p = 0.0392 |
| | Genotype × trial | F(14,392) = 0.76 | p = 0.7082 |
| | Genotype × sex | F(2,56) = 0.29 | p = 0.7499 |
| Tukey HSD | Shank3+/+CreTam+ vs | Shank3+/GCreTam+ | p = 0.9642 |
| | Shank3+/+CreTam+ vs | Shank3G/GCreTam+ | p = 0.9906 |
| | Shank3+/GCreTam+ vs | Shank3G/GCreTam+ | p = 0.9351 |
fEPSP slope | Two-way rmANOVA | Genotype | F(1,26) = 0.34 | p = 0.5634 |
(Fig. 7F) | | Intensity | F(7,182) = 29.71 |
*p < 0.0001 |
| | Genotype × intensity | F(7,182) = 6.30 |
*p < 0.0001 |
Fiber volley | Two-way rmANOVA | Genotype | F(1,11) = 0.46 | p = 0.5135 |
| | Intensity | F(7,77) = 24.06 |
*p < 0.0001 |
| | Genotype × intensity | F(7,77) = 0.16 | p = 0.9915 |
Vehicle-treated Shank3GCreTam+ mice |
Nest width | Three-way rmANOVA | Genotype | F(2,56) = 2.33 | p = 0.1065 |
(Fig. 8A) | | Time | F(2,112) = 34.73 |
*p < 0.0001 |
| | Sex | F(1,56) = 0.44 | p = 0.5100 |
| | Genotype × time | F(4,112) = 0.91 | p = 0.4630 |
| | Genotype × sex | F(2,56) = 2.13 | p = 0.1287 |
| Tukey HSD | Shank3+/+CreTam+ vs | Shank3+/GCreTam+ | p = 0.6704 |
| | Shank3+/+CreTam+ vs | Shank3G/GCreTam+ | p = 0.0637 |
| | Shank3+/GCreTam+ vs | Shank3G/GCreTam+ | p = 0.3149 |
Nest height | Three-way rmANOVA | Genotype | F(2,56) = 2.72 | p = 0.0749 |
(Fig. 8B) | | Time | F(2,112) = 72.06 |
*p < 0.0001 |
| | Sex | F(1,56) = 0.59 |
*p = 0.0446 |
| | Genotype × time | F(4,112) = 1.32 | p = 0.2687 |
| | Genotype × sex | F(2,56) = 0.82 | p = 0.4466 |
| Tukey HSD | Shank3+/+CreTam+ vs | Shank3+/GCreTam+ | p = 0.6704 |
| | Shank3+/+CreTam+ vs | Shank3G/GCreTam+ | p = 0.0637 |
| | Shank3+/GCreTam+ vs | Shank3G/GCreTam+ | p = 0.3149 |
Marble burying | Two-way ANOVA | Genotype | F(2,55) = 15.78 |
*p < 0.0001 |
(Fig. 8C) | | Sex | F(1,55) = 0.50 | p = 0.4810 |
| | Genotype × sex | F(2,55) = 0.12 | p = 0.8874 |
| Tukey HSD | Shank3+/+CreTam+ vs | Shank3+/GCreTam+ |
*p = 0.0005 |
| | Shank3+/+CreTam+ vs | Shank3G/GCreTam+ |
*p = 0.0001 |
| | Shank3+/GCreTam+ vs | Shank3G/GCreTam+ | p = 0.3265 |
Locomotor | Three-way rmANOVA | Genotype | F(2,56) = 2.48 | p = 0.0926 |
(Fig. 8D) | | Time | F(23,1288) = 101.49 |
*p < 0.0001 |
| | Sex | F(1,56) = 1.98 | p = 0.1644 |
| | Genotype × time | F(46,1288) = 2.57 |
*p < 0.0001 |
| | Genotype × sex | F(2,56) = 0.11 | p = 0.8946 |
| Tukey HSD | Shank3+/+CreTam+ vs | Shank3+/GCreTam+ | p = 0.0842 |
| | Shank3+/+CreTam+ vs | Shank3G/GCreTam+ | p = 0.9893 |
| | Shank3+/GCreTam+ vs | Shank3G/GCreTam+ | p = 0.1944 |
Rotarod | Three-way rmANOVA | Genotype | F(1,56) = 0.12 | p = 0.8894 |
(Fig. 8E) | | Trial | F(7,392) = 17.89 |
*p < 0.0001 |
| | Sex | F(1,56) = 4.46 |
*p = 0.0392 |
| | Genotype × trial | F(14,392) = 0.76 | p = 0.7082 |
| | Genotype × sex | F(2,56) = 0.29 | p = 0.7499 |
| Tukey HSD | Shank3+/+CreTam+ vs | Shank3+/GCreTam+ | p = 0.9642 |
| | Shank3+/+CreTam+ vs | Shank3G/GCreTam+ | p = 0.9906 |
| | Shank3+/GCreTam+ vs | Shank3G/GCreTam+ | p = 0.9351 |
fEPSP slope | Two-way rmANOVA | Genotype | F(1,30) = 0.26 | p = 0.6130 |
(Fig. 8F) | | Intensity | F(7,210) = 52.49 |
*p < 0.0001 |
| | Genotype × intensity | F(7,210) = 0.45 | p = 0.8699 |
Fiber volley | Two-way rmANOVA | Genotype | F(1,15) = 1.80 | p = 0.1995 |
| | Intensity | F(7,105) = 155.78 |
*p < 0.0001 |
| | Genotype × intensity | F(7,105) = 2.96 |
*p = 0.0072 |
|
Effect of CAG-CreTam on Shank3+/+ mice |
Nest height | Three-way rmANOVA | CAG-CreTam | F(1,49) = 3.12 | p = 0.0837 |
| | Time | F(2,98) = 44.08 |
*p < 0.0001 |
| | Sex | F(1,49) = 3.83 | p = 0.0559 |
| | CAG-CreTam × time | F(2,98) = 1.65 | p = 0.1972 |
| | CAG-CreTam × sex | F(2,549 = 0.19 | p = 0.6674 |
Nest width | Three-way rmANOVA | CAG-CreTam | F(1,49) = 0.08 | p = 0.7752 |
| | Time | F(2,98) = 27.22 |
*p < 0.0001 |
| | Sex | F(1,49) = 3.37 | p = 0.0725 |
| | CAG-CreTam × time | F(2,98) = 1.28 | p = 0.2839 |
| | CAG-CreTam × sex | F(2,49) = 0.299 | p = 0.5872 |
Marble | Two-way ANOVA | CAG-CreTam | F(1,49) = 0.34 |
*p = 0.5654 |
Burying | | Sex | F(1,49) = 2.93 | p = 0.0934 |
| | CAG-CreTam × sex | F(1,49) = 0.43 | p = 0.5127 |
Locomotor | Three-way rmANOVA | Genotype | F(1,49) = 16.00 |
*p = 0.0002 |
| | Time | F(23,1127) = 110.93 |
*p < 0.0001 |
| | Sex | F(1,49) = 2.38 | p = 0.1293 |
| | CAG-CreTam × time | F(23,1127) = 1.46 | p = 0.0749 |
| | CAG-CreTam × sex | F(1,49) = 0.19 | p = 0.6626 |
Rotarod | Three-way rmANOVA | CAG-CreTam | F(1,49) = 0.21 | p = 0.6472 |
| | Trial | F(7,343) = 19.90 |
*p < 0.0001 |
| | Sex | F(1,49) = 1.99 | p = 0.1651 |
| | CAG-CreTam × trial | F(7,343) = 0.76 | p = 0.6180 |
| | CAG-CreTam × sex | F(1,49) = 0.79 | p = 0.5973 |
fEPSP slope | Two-way rmANOVA | CAG-CreTam | F(1,24) = 86.39 |
*p < 0.0001 |
(Fig. 9A) | | Intensity | F(7,168) = 58.00 |
*p < 0.0001 |
| | CAG-CreTam × intensity | F(7,168) = 3.60 |
*p = 0.0012 |
Fiber volley | Two-way rmANOVA | CAG-CreTam | F(1,9) = 0.69 | p = 0.4270 |
| | Intensity | F(7,63) = 56.18 |
*p < 0.0001 |
| | CAG-CreTam × intensity | F(7,63) = 2.95 |
*p = 0.0098 |
Effect of CAG-CreTam on Shank3G/
G mice |
Nest height | Three-way rmANOVA | CAG-CreTam | F(1,30) = 0.33 | p = 0.5693 |
| | Time | F(2,60) = 21.20 |
*p < 0.0001 |
| | Sex | F(1,30) = 1.94 | p = 0.1736 |
| | CAG-CreTam × time | F(2,60) = 0.38 | p = 0.6865 |
| | CAG-CreTam × sex | F(2,60) = 0.44 | p = 0.5132 |
Nest width | Three-way rmANOVA | CAG-CreTam | F(1,30) = 0.12 | p = 0.7331 |
| | Time | F(2,60) = 20.06 |
*p < 0.0001 |
| | Sex | F(1,30) = 3.37 | p = 0.0762 |
| | CAG-CreTam × time | F(2,60) = 0.08 | p = 0.9219 |
| | CAG-CreTam × sex | F(2,60) = 0.02 | p = 0.8884 |
Marble | Two-way ANOVA | CAG-CreTam | F(1,30) = 0.00 | p = 0.9828 |
Burying | | Sex | F(1,30) = 0.40 | p = 0.5294 |
| | CAG-CreTam × sex | F(1,30) = 1.03 | p = 0.3179 |
Locomotor | Three-way rmANOVA | CAG-CreTam | F(1,30) = 11.83 |
*p = 0.0017 |
| | Time | F(23,690) = 36.91 |
*p < 0.0001 |
| | Sex | F(1,30) = 0.05 | p = 0.8193 |
| | CAG-CreTam × time | F(23,690) = 0.89 | p = 0.6145 |
| | CAG-CreTam × sex | F(1,30) = 0.18 | p = 0.6777 |
Rotarod | Three-way rmANOVA | CAG-CreTam | F(1,30) = 5.99 |
*p = 0.0205 |
| | Trial | F(7,210) = 4.39 |
*p < 0.0001 |
| | Sex | F(1,30) = 1.99 | p = 0.1682 |
| | CAG-CreTam × trial | F(7,210) = 1.00 | p = 0.4323 |
| | CAG-CreTam × sex | F(1,30) = 1.42 | p = 0.2422 |
fEPSP slope | Two-way rmANOVA | CAG-CreTam | F(1,23) = 0.58 | p = 0.4527 |
(Fig. 9B) | | Intensity | F(7,161) = 33.71 |
*p < 0.0001 |
| | CAG-CreTam × intensity | F(7,161) = 0.173 | p = 0.9904 |
Fiber volley | | CAG-CreTam | F(1,8) = 0.20 | p = 0.6695 |
| | Intensity | F(7,56) = 28.25 |
*p < 0.0001 |
| | CAG-CreTam × intensity | F(7,56) = 2.67 |
*p = 0.0184 |